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1998 (5)

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The yeasts, a taxonomic study
Authors: ---
ISBN: 9780444813121 0444813128 0080542697 9780080542690 9786611038991 1281038997 Year: 1998 Publisher: Amsterdam ; New York : Elsevier,

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Abstract

The yeasts are a phylogenetically diverse group of fungi characterized by unicellular growth. Yeasts have been used for bread making and brewing beverages for millennia, and have become increasingly important in biotechnology for production of fuel alcohol, organic acids, enzymes, and various pharmacologically important chemicals. Other species are serious human, animal, and plant pathogens. Since publication of the 3rd edition of this book in 1984, numerous new species and genera have been described, many because of the application of new molecular biological methods. Molecular comparisons

Methods in yeast genetics: a Cold Spring Harbor Laboratory course manual
Authors: --- ---
ISBN: 0879695080 9780879695088 Year: 1998 Publisher: Cold Spring Harbor (N.Y.): Cold Spring Harbor Laboratory,

Pichia Protocols
Authors: ---
ISBN: 0896034216 9786610843077 1280843071 1592595782 Year: 1998 Volume: 103 Publisher: Clifton, NJ : Humana Press,

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Abstract

The Pichia pastoris expression system has rapidly become one of the key protein expression systems used in commercial, academic, and government laboratories today. In Pichia Protocols, David Higgins and James Cregg compile for the first time an all-inclusive collection of powerful experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods-developed by world-class researchers in their own laboratories-contain detailed descriptions of available expression strains, as well as vectors, and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Pichia Protocols captures the collective expertise of the international P. pastoris scientific community in close detail. It conveys a breadth of experience that will permit any researcher in molecular biology to quickly construct an expression strain designed to produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today.

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