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Eukaryotic DNA Replication: A Practical Approach is a comprehensive practical manual, with each of its eleven chapters describing an aspect of the methods currently used to investigate DNA replication in eukaryotes. The sequence of the chapters corresponds roughly to the order of events during DNA replication. The first chapters are concerned with initiation, looking at methods to characterize origins of replication and the proteins that interact with them. There then follow chapters describing protocols for the study of the elongation phase and the synthesis of the telomeres. The final chapters provide a more general overview of the study of DNA replication - including its investigation in model systems such as yeast, xenopus and viruses, and looks into methods used to study DNA:protein interactions that could be applied to the study of replication proteins. This exciting new volume provides over 120 tried and tested protocols for the analysis of eukaryotic DNA replication and will be of major interest to a wide variety of molecular and cell biologists, biochemists and medical researchers.

DNA replication. --- Chromosomal DNA replication --- DNA --- Replication of DNA --- Replication --- Synthesis

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DNA Viruses: A Practical Approach groups together the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and realtime PCR analysis of drug resistance mutations in clinical isolates. Chapter 1 provides an overview of the extraction, purification and characterizations of virus DNA, but also covers the fundamentals of DNA virus culture. Chapters 2 and 3 describe approaches to the molecular investigation and mutagenesis of DNA virus genomes. Chapter 4 considers DNA virus replication and then chapters 5 & 6 describe methods to study transcription control. Chapters 7 to 9 consider aspects of the pathogenesis of DNA virus infections. The final chapter describes the current technology being applied to the development of DNA virus vectors for gene delivery. This volume will therefore be of interest to all those working on DNA viruses whether in academia, industry or clinical research.

DNA viruses. --- Deoxyribonucleic acid viruses --- Viruses, DNA --- Viruses --- General virology --- PCR (polymerase chain reaction) --- mutageniteit --- virologie --- DNA (deoxyribonucleic acid) --- moleculaire biologie --- spectrometrie --- Dna virus infections --- Dna viruses --- Dna viruses, isolation & purification. --- Virology. --- Isolation & purification. --- Physiology.

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DNA ADDUCTS --- FISHES --- PHOSPHORUS ISOTOPES --- ISOTOPE LABELING

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genes --- DNA. --- DNA --- population genetics --- Bacteria --- cloning --- genetic maps --- genetic transformation --- genetic code --- Genetique mendelienne

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Aging --- Mitochondrial pathology. --- Mitochondrial DNA --- Free radicals (Chemistry) --- DNA, Mitochondrial --- Aging. --- Free Radicals --- Lipids