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Few would have predicted 20 years ago that myosins constitute a superfamily with at least two-dozen classes and that these molecular motors are involved in a multitude of intracellular activities including cell division, cell movement, intracellular transport and signal transduction. Application of state-of-the-art cellular and molecular biological, structural biological, genetic, biochemical and biophysical techniques has provided and continues to provide critical information regarding the structure–function relationship; and the cellular roles of various myosins in organisms as diverse as protozoa, yeast, plants and higher animals. The association of myosins with diseases including neurological disorders, immu- deficiencies, cardiomyopathies, hearing and vision loss testify to the importance of understanding the biochemical properties and cellular roles of myosins. The 16 chapters in this volume summarize the tremendous progress made in studying members of the myosin superfamily in recent years and offer critical insight into what future research will yield. I would like to express my sincere gratitude to the authors of this volume. It was a pleasure to work with each of you and I thank you for the considerable efforts in making this international endeavor possible. I also thank John Trinick from the University of Leeds, UK, for the elegant montage of images of single molecules of myosins on the cover, which beautifully shows the structures of some of these amazing molecules. The able assistance of Marlies Vlot from Springer and Indumadhi Srinivasan from Integra Software Services for production of the book is greatly appreciated.

Muscle proteins. --- Myosin. --- Proteins --- Microfilament proteins --- Muscle proteins

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Muscle proteins --- Physiology

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect - full-text online of volumes 1 onw

Globin. --- Proteins. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Hemoglobin --- Histones --- Proteins

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Biofluorescence. --- Fluorescent polymers. --- Proteins. --- Green Fluorescent Proteins. --- Neurobiology --- Methods.

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viruses. --- Leukosis --- Neuromuscular diseases --- Hybrid proteins --- Binding proteins --- Recombinant proteins --- lymphocytes --- Drug therapy --- replication. --- DNA --- Chronic course --- Chronic course