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This volume explores a collection of experimental techniques used to investigate different aspects of pollen development and function, including its role in reaching the ovule and delivering the two sperm cells. The techniques discussed range from basic methodology to cultivate pollen in vitro to the sophisticated experiments involving micromanipulation, Lab-on-Chip technology, or high-end imaging. The chapters in this book cover topics such as pollen grain counting using a cell counter; restricted pollination for tracing individual pollen tubes in a pistil; obtaining mutant pollen for phenotypic analysis and pollen tube dual-staining; analyzing intracellular gradients in pollen tubes; and measuring exocytosis rate in Arabidopsis pollen tube using corrected fluorescence recovery after photoconversion (cFRAPc) technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Pollen and Pollen Tube Biology: Methods and Protocols is a valuable resource for novice and expert scientists interested in learning more about the field of plant reproduction. .

Botany. --- Plant Science. --- Botanical science --- Floristic botany --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Plants

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This volume provides a comprehensive overview of different ways to grow algae and the techniques used to start algal cultivation, monitor algal growth, environmental impact of growing algae, and various methods for characterizing the biomass. The chapters in this book discuss a range of topics such as step-by-step protocols on how to isolate and grow algae; descriptions on how to use fluorescence to estimate algal biomass and lipid content; and instructions on using advanced techniques to determine the carbohydrates and lipids in algal biomass. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Biofuels from Algae: Methods and Protocols is a valuable resource for novice and experienced researchers who want to learn more about this impactful and developing field.

Biotechnology. --- Botany. --- Plant Science. --- Botanical science --- Floristic botany --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Plants --- Chemical engineering --- Genetic engineering

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This third edition details recent advances made in the field of proteomics and the application to plant biology and translational research. In recent years, improvements have been made to protocols on high-throughput proteomics at all workflow stages, from wet to dry labs. Chapters are divided into nine parts, covering methods and protocols such as applications of gel-free, label- or label-free, imaging and targeted approaches to experimental model systems, crops and orphan species, study and analysis of PTMs, protein interactions, specific families of proteins, and finally proteomics in translational research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Proteomics: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

Botany. --- Proteins. --- Plant Science. --- Protein Biochemistry. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Botanical science --- Floristic botany --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Plants

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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact

Science: general issues --- Medical genetics --- genomics --- bioinformactics --- systems biology --- plant science --- gene expression --- special issue

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This book will cover the entire evolutionary history that the terrestrial plants have recorded in Brazilian sedimentary rocks, ranging from the first vestiges of terrestrial environments colonization about 400 million years ago, until reaching the eve of the present time, when the current vegetation formations were organizing to reach their current distribution in modern biomes. At present Brazil is home to the world’s greatest plant biodiversity and we aim to offer here an opportunity to appreciate how this floral biodiversity originated and developed in these lowlands of South America, through chapters elaborated by the best Brazilian and foreign experts who dedicate to elucidate the evolution of the ancient flora in this part of the planet.

Plant science. --- Botany. --- Paleontology . --- Ecology . --- Climatology. --- Paleoecology. --- Plant Sciences. --- Paleontology. --- Ecology.