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Molecular probes. --- Bioprobes --- Probes, Molecular --- Molecular biology --- Affinity labeling --- Technique

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Analytical biochemistry --- Fysicochemical separation methods --- Affinity chromatography --- Chemical affinity --- Proteins --- Affinity labeling

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Concentrating on the aspects of protein function that are common to the majority of proteins, this collection of methods is brought together for reseachers who are without access to expensive equipment. Using these protocols researchers will be able to get information about the functional properties of any protein. A companion volume, "Protein Structure: A Practical Approach" also edited by Thomas Creighton, provides the methods necessary for the study of protein strucutre.

biochemie --- General biochemistry --- biochemistry --- proteïnen --- Relations structure-activité --- Affinity labeling --- Structure-activity relationships --- Affinity labeling. --- Structure-activity relationships. --- Relations structure-activité --- Biochemistry --- Proteins --- #WSCH:WBIO --- 577.122 --- 577.122 Protein metabolism --- Protein metabolism --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Protein affinity labeling --- Protein binding --- Technique --- Analysis --- physiology --- Basic Sciences. Chemistry --- Technique. --- Analysis. --- physiology. --- Proteins and Enzymes. --- Biochimie --- Protéines --- Analyse --- Electrophoresis. --- Molecular cloning. --- DNA. --- Physiology. --- Biochemistry - Technique --- Proteins - Structure-activity relationships --- Proteins - Affinity labeling --- Proteins - physiology --- DNA --- Genes --- Molecular structure-biological activity relationship

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The analysis of protein function is a vital step in the characterization of any newly discovered protein. This new edition brings up to date the techniques used, and presents experimental procedures that can be performed in the average laboratory without recourse to highly specialised equipment. The protocols will be of use to both experienced and novice researchers and are accompanied by background information, hints and tips, and troubleshooting guides to ensure successful elucidation of protein function.

Proteins --- Protein binding --- Protéines --- physiology. --- Analysis. --- Research --- Methodology. --- Structure-activity relationships. --- Affinity labeling. --- Analyse --- Fixation --- Recherche --- Méthodologie --- Proteins. --- Protéines --- Méthodologie --- Physiology. --- LABORATORY MANUALS --- PROTEINS --- ESCHERICHIA COLI --- STRUCTURES --- DNA-BINDING PROTEINS --- GENE EXPRESSION --- PROTEIN FOLDING --- LIGAND BINDING --- PROTEIN SEQUENCE DETERMINATION --- CROSSLINKING --- AFFINITY LABELS --- IDENTIFICATION --- TECHNIQUES --- RECOMBINANT PROTEINS --- PRODUCTION --- ELECTROPHORESIS --- LIGANDS --- BINDING SITES --- LABELLING --- CHEMICAL STRUCTURE --- ANALYSIS

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This new edition provides the most advanced research using bioprobes on the chemical control of 1) cell cycle and differentiation, 2) epigenetics, 3) apoptosis and autophagy, and 4) immune response. The “bioprobe”, first proposed in the first edition, has become an indispensable tool for chemical biology and has substantially assisted in the investigation of complex biochemical processes of cells. New areas of investigation such as stem cell research, epigenetic research, and autophagy research have rapidly advanced in the past 10 years. Including these new findings, this second edition supplies up-to-date information on the biochemical tools called bioprobes. Data on each bioprobe, such as chemical structure, origin, function, and references, are presented as one item in this volume. Readers will easily find useful information and will be able to determine the appropriate bioprobes to investigate cell functions. The information on bioprobes and their use in research makes this book a valuable source for researchers in diverse fields. Not only scientists in academia but also in the pharmaceutical industries will discover the most important information about small molecules useful for drug discovery.

Life sciences. --- Biotechnology. --- Organic chemistry. --- Biochemistry. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Biochemistry, general. --- Organic Chemistry. --- Molecular probes. --- Bioprobes --- Probes, Molecular --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Organic chemistry --- Chemical engineering --- Genetic engineering --- Biosciences --- Sciences, Life --- Science --- Composition --- Molecular biology --- Affinity labeling --- Technique --- Cytology. --- Chemistry, Organic.