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Biotransformation has accompanied mankind since the Neolithic community, when people settled down and began to engage in agriculture. Modern biocatalysis started in the mid-1850s with the pioneer works of Pasteur. Today, biotransformations have become an indispensable part of our lives, similar to other hi-tech products. Now, in 2019, biocatalysis “received” the Nobel Prize in Chemistry due to prof. Frances H. Arnold’s achievements in the area of the directed evolution of enzymes. This book deals with some major topics of biotransformation, such as the application of enzymatic methods in glycobiology, including the synthesis of hyaluronan, complex glycoconjugates of N-acetylmuramic acid, and the enzymatic deglycosylation of rutin. Enzymatic redox reactions were exemplified by the enzymatic synthesis of indigo from indole, oxidations of β-ketoesters and the engineering of a horse radish peroxidase. The enzymatic reactions were elegantly employed in biosensors, such as glucose oxidase, in the case of electrochemical glucose sensors. Nitrilases are important enzymes for nitrile metabolism in plants and microorganisms have already found broad application in industry—here, these enzymes were for the first time described in Basidiomyceta. This book nicely describes molecular biocatalysis as a pluripotent methodology—“A jack of all trades...”—which strongly contributes to the high quality and sustainability of our daily lives.

E. coli --- recombinant horseradish peroxidase --- site-directed mutagenesis --- periplasm --- glycosylation sites --- Aspergillus niger --- quercetin --- rutin --- rutinose --- rutinosidase --- “solid-state biocatalysis” --- hyaluronic acid --- in vitro synthesis --- one-pot multi-enzyme --- optimization --- enzyme cascade --- Basidiomycota --- Agaricomycotina --- nitrilase --- cyanide hydratase --- nitrile --- substrate specificity --- overproduction --- homology modeling --- substrate docking --- phylogenetic distribution --- indigo --- MISO library --- flavin --- monooxygenase --- FMO --- β-N-acetylhexosaminidases --- transglycosylation --- Glide docking --- Talaromyces flavus --- muramic acid --- non-reducing carbohydrate --- glucose oxidase --- direct electron transfer --- amine-reactive phenazine ethosulfate --- glucose sensor --- glycemic level monitoring --- Pseudomonas putida MnB1 --- biogenic manganese oxides --- abiotic manganese oxides --- α-Hydroxy-β-keto esters --- whole-cell biocatalysis --- surface display --- cell wall anchor --- Lactobacillus plantarum --- whole-cell biocatalyst --- n/a --- Fe(II)/2-ketoglutarate-dependent dioxygenase --- 2-ketoglutarate generation --- regio- and stereo-selective synthesis --- hydroxy amino acids --- sequential cascade reaction --- "solid-state biocatalysis"

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The scope of this Special Issue is to collect some of the contributions to the First International Electronic Conference on Biosensors, which was held to bring together well-known experts currently working in biosensor technologies from around the globe, and to provide an online forum for presenting and discussing new results. The world of biosensors is definitively a versatile and universally applicable one, as demonstrated by the wide range of topics which were addressed at the Conference, such as: bioengineered and biomimetic receptors; microfluidics for biosensing; biosensors for emergency situations; nanotechnologies and nanomaterials for biosensors; intra- and extracellular biosensing; and advanced applications in clinical, environmental, food safety, and cultural heritage fields.

Listeria monocytogenes --- cell-based biosensor --- bioelectric recognition assay --- membrane-engineering --- lateral flow immunoassay --- antibiotics --- lincomycin --- gold nanoparticles --- quantum dots --- surface-enhanced Raman spectroscopy --- Fumonsin B1 --- aptamers --- UV/VIS spectroscopy --- asymmetric flow field-flow fractionation --- porous silicon membrane --- bacterial detection --- selective lysis --- endolysins --- lysostaphin --- flow-through --- vibrational spectra --- molecular dynamics --- nucleotides --- Au nanoparticle --- SERS --- space biology --- deep space --- biosensors --- space radiation --- microgravity --- CubeSats --- 2D Ti3C2 MXene --- PGE2 --- 8-HOA --- lung cancer --- electrochemical --- DNA biosensors --- KRAS --- liquid biopsy --- cancer point-of-care diagnostic tests --- paper-based sensors --- nitrocellulose --- impedance measurements --- dielectric properties --- parallel-plate electrodes --- interdigital electrodes --- Bacillus thuringiensis --- sensor --- combined slot antenna --- diabetes --- dielectric permeability --- electromagnetic fields --- glucose concentration --- near-field sensor --- non-invasive measurements --- microwave sounding --- chymotrypsin --- β-casein --- nanoparticles --- UV-vis spectroscopy --- dynamic light scattering --- quartz crystal microbalance --- biologically inspired --- electronic eye --- optical methods --- RGB analysis --- tequila --- molecularly imprinted polymer (MIP) --- surface plasmon resonance (SPR) --- plastic optical fiber (POF) --- 2-furaldheide (2-FAL) --- beverages --- optical chemical sensors --- biosensor --- immunosensor --- cardiac troponin I --- single-strand DNA --- electrochemical impedance spectroscopy --- label-free --- proteins --- microfluidic chip --- self-assembled monolayers --- odor sensor --- market analysis --- technology assessment --- application field --- performance profile --- requirement profile --- biointelligence --- biological transformation --- temperature sensor --- Raman spectroscopy --- anti-Stokes/Stokes spectra --- titanium dioxide --- trypsin --- AuNPs --- acoustic wave biosensor --- colorimetric assay --- white light reflectance spectroscopy --- real-time immunosensor --- ELISA --- pesticides --- carbendazim --- fruit juices --- ECIS --- xCELLigence --- cellZscope --- hCMVEC --- endothelial cell --- impedance sensing --- light-addressable potentiometric sensor --- light-addressable electrode --- actuator-sensor system --- enzyme kinetics --- microfluidics --- HIV-1 p24 protein --- surface plasmon resonance --- surface modifications --- label-free detection --- artificial enzymes --- green synthesis --- hexacyanoferrates of transition and noble metals --- peroxidase mimetic --- amperometric (bio)sensor --- glucose oxidase --- glucose analysis --- electrocatalysis --- peroxynitrite --- flow injection analysis --- meat extracts --- myoglobin --- cobalt phthalocyanine --- electrochemical reduction --- screen-printed carbon electrode --- amperometric detection --- decay kinetics