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This Test Guideline describes a laboratory test method to assess abiotic hydrolytic transformations of chemicals in aquatic systems at pH values normally found in the environment (pH 4 – 9). This Guideline is designed as a tiered approach; each tier is triggered by the results of the previous tier.Sterile aqueous buffer solutions of different pH values (pH 4, 7 and 9) are treated with the non-labelled or labelled test substance (only one concentration, which should not exceed 0.01 M or half of the saturation concentration). They are incubated in the dark under controlled laboratory conditions (at constant temperatures). After appropriate time intervals, buffer solutions are analysed for the test substance and for hydrolysis products. The preliminary test should be carried out for 5 days at 50 ± 0.5°C and pH 4.0, 7.0 and 9.0. The second tier consists of the hydrolysis of unstable substances, and the third tier is the identification of hydrolysis products. The higher Tier tests should be conducted until 90 % hydrolysis of the test substance is observed or for 30 days whichever comes first.

Chemical tests and reagents --- Chemical reagents --- Reagents, Chemical --- Indicators and test-papers

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The workshop report reviews the options available for the analysis of data from ecotoxicity tests; compares their advantages and disadvantages; recommends (a) the most appropriate approach for deriving a summary parameter(s) which has (have) scientific validity and (b) further work to be undertaken by the OECD and/or others, as appropriate.

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The purpose of the Dominant lethal (DL) test is to investigate whether chemical agents produce mutations resulting from chromosomal aberrations in germ cells. In addition, the dominant lethal test is relevant to assessing genotoxicity because, although they may vary among species, factors of in vivo metabolism, pharmacokinetics and DNA-repair processes are active and contribute to the response. Induction of a DL mutation after exposure to a test chemical indicates that the chemical has affected germinal tissue of the test animal. This modified version of the Test Guideline reflects more than thirty years of experience with this test and the potential for integrating or combining this test with other toxicity tests such as developmental, reproductive toxicity, or genotoxicity studies; however due to its limitations and the use of a large number of animals this assay is not intended for use as a primary method, but rather as a supplemental test method which can only be used when there is no alternative for regulatory requirements.

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Cette Ligne directrice décrit des méthodes pour déterminer la stabilité de stockage d'une substance par rapport à la chaleur et à l'air. Deux méthodes s'appliquent aux substances homogènes solides et liquides ainsi qu’à leurs mélanges : l'essai de stockage accéléré et les méthodes d'analyse thermique.Dans l’essai de stockage accéléré (CIPAC), une instabilité lors d’un stockage de longue durée peut être simulée en appliquant une température plus élevée pendant un essai court. Cet essai consiste à stocker la substance étudiée entre 54°C et 55°C pendant 14 jours puis à l'analyser. Dans des cas simples, il sera suffisant de déterminer une propriété caractéristique avant et après le stockage. La substance est considérée stable à la température ambiante si le point de fusion est resté constant ou si la teneur en substance originale déterminée par analyse n’a pas diminué de plus de 5 pour cent. Dans les méthodes d'analyse thermique (ATG et ATD), l'échantillon et le produit de référence sont chauffés, avec une élévation constante, jusqu'à la température finale dans une atmosphère d'essai définie, soit séparément dans un appareillage ATG ou ATD, soit dans un système combiné. Les modifications de poids de l'échantillon ou les quantités de la chaleur absorbées ou dégagées sont mesurés et enregistrés. La substance est considérée stable à la température ambiante si aucune décomposition ou transformation chimique n'est trouvée en dessous de 150°.

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This detailed review documents examines member country classification of substances and mixtures which cause respiratorytract  irritation and corrosion.

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