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With the 'post genomics' era comes an increasing demand for the techniques of cell biology, critical to interpreting the function and location of the cell's myriad proteins and macromolecules. In response, this second edition of Plant Cell Biology balances established techniques, including classical histochemistry and electron microscopy, with new developments in the field. The book covers a substantial range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green fluorescent protein, micromanipulation and electrophysiological techniques. Also featured are chapters on macromolecular location procedures involving immunocytochemistry and in situ hybridisation, and the book concludes with a range of biochemical techniques for the isolation of cytoplasmic organelles. The book provides advanced students, postgraduates and researchers in the plant sciences with an invaluably comprehensive guide to the ever-growing field of plant cell biology.
Botanical microscopy --- Plant cytochemistry --- Plant cells and tissues. --- Technique. --- Plant cells and tissues
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Immunocytochemistry of Plant Cells is the first book exclusively dedicated to this topic. The first and largest portion of the book is concerned with a group of proven protocols and variations on these protocols that might prove useful, many developend or modified in the author's laboratory. The second portion of the book covers the studies that have been published previously on each of the plant organelles. Numerous state of the art micrographs from researchers around the world are included to demonstrate typical results.
Photosystem. --- Plant cells. --- Polymerization. --- Plant cytochemistry --- Immunocytochemistry --- Botany --- Earth & Environmental Sciences --- Plant Anatomy --- Plant Physiology --- Immunocytochemistry. --- Plant cells and tissues. --- Plant tissues --- Life sciences. --- Antibodies. --- Cell biology. --- Plant anatomy. --- Plant development. --- Biology --- Life Sciences. --- Plant Anatomy/Development. --- Cell Biology. --- Biological Techniques. --- Technique. --- Cells --- Plant anatomy --- Tissues --- Cytochemistry --- Immunochemistry --- Immunofluorescence --- Monoclonal antibodies. --- Cytology. --- Cytology --- Research --- Methodology. --- Cell biology --- Cellular biology --- Cytologists --- Antibodies, Monoclonal --- Monoclonal immunoglobulins --- Immunoglobulins --- Molecular cloning --- Plant structure --- Plants --- Structural botany --- Vegetable anatomy --- Anatomy --- Structure --- Biology—Technique. --- Antibodies --- Immune globulins --- Immune serum globulin --- Blood proteins --- Globulins --- Plasma cells --- Antibody diversity --- Antigens --- Bacterial immunoglobulin-binding proteins --- Development of plants --- Plant development --- Developmental biology --- Growth (Plants) --- Ontogeny
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