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This edited volume discusses the identification, discovery, characterization, structure determination and modeling of multicomponent macromolecular complexes, and as such, it fully complements the first volume (ISBN 978-3-319-27214-6), which targeted methods of recombinant production of protein complexes. This book is divided in 8 sections offering a selection of technologies widely used in the characterization of protein and protein-nucleic acid complexes for different purposes and at different scales. From native electrophoresis methods, that are accessible to any reasonably well-equipped laboratory, to the sophisticated setup required for structure determination by cryo-electron microscopy or X-ray crystallography, this book contains a wide variety of clearly explained analytic and preparative approaches, connected with the production techniques developed on the previous volume. The readers will find an integral connection between this book and the first volume, which ensures a comprehensive and updated discussion of the main topics of the discipline. Taken together, these volumes constitute a cohesive and authoritative source of the research on multicomponent macromolecular complexes. In here, we focus on characterization of protein complexes in the broadest sense, which is not typically covered in other sources. Moreover, all chapters are carefully written by world renowned scientists and active researchers, making this volume ideal, not only as a reference source, but also as a companion book for the daily laboratory work. This book is aimed for a wide range of scientists, from science students to experienced researchers, working on protein and protein-nucleic acid complexes, who need a thorough understanding of protein production and complex characterization. .

Life sciences. --- Life Sciences.

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The cultivation and harvesting of sugar beets (roots and haulm) is one of the most labour and energy consuming work processes in the agricultural industry. The improvement of the qualitative indicators of the sugar beet harvesting process as the final operation in the work flow of its production represents a multi-faceted research-and-engineering problem, which is to be solved basing on the search for new implement design concepts and overall beet harvester design layouts, the thorough theoretical substantiation of their design and process parameters, the experimental verification of the completed theoretical research with the ultimate objective of the analysis and synthesis of their rational parameters. Theoretical research must play a fundamental role in the mechanical and technological substantiation of the root lifting process. It must be used as the basis for developing rational kinematic and dynamic operation conditions in order to achieve the required quality of the performed work process as well as the streamlined energy consumption. The book can be considered as one of the first solid analytical studies of the process of vibrational digging of sugar beet roots. It offers the analysis of up-to-date engineering solutions of vibrational digging tools and experimental investigations of the first vibrational lifters, the equation of oscillating motions of digging shares.

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Pichi Sermolli's work with his more than 2750 collections of plants from nearly 150 localities on the Lake Tana expedition in Ethiopia in 1937 was interrupted by World War II, but completed in 1947 at the Royal Botanic Gardens, Kew, and the British Museum (Natural History), UK. It resulted in preliminary accounts of the vegetation published 1938-40 and a taxonomically arranged account in 1951, all in Italian. Pichi Sermolli's observations are difficult to locate due to the imperfect maps of the time, but in this book the authors have reconstructed the sequence of the collections, georeferenced the localities, and updated the identifications of the species. By reconstructing Pichi Sermolli's observations, it is possible to draw conclusions about the vegetation and compare with a recent model of the vegetation of Ethiopia. According to this, the vegetation of the Lake Tana Basin was a complex mosaic of woodland, scrub, forest, farmland and lake shore vegetation now difficult to interpret in detail. Pichi Sermolli's study of the vegetation in the Semien Mountains demonstrated for the first time the zonation of Ericaceous woodland and Afroalpine vegetation, within which he distinguished Carex monostachya bogs, Afroalpine grasslands with Lobelia rhynchopetalum, and stony and rocky Afroalpine vegetation. This book interprets Pichi Sermolli's observations in English and compares them with modern knowledge of the region, partly obtained by the present authors' own field work. It demonstrates how Pichi Sermolli's studies form a valuable contribution to the understanding of the Ethiopian flora and vegetation.

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Population dynamics constitutes a widespread branch of investigations which finds important applications within the realm of life science. The classical deterministic (macroscopic) approach aims at characterizing the time evolution of families of homologous entities, so to unravel the global mechanisms which drive their dynamics. As opposed to this formulation, a microscopic level of modeling can be invoked which instead focuses on the explicit rules governing the interactions among individuals. A viable tool that enables to bridge the gap between the two approaches is the van Kampen's system size expansion. In this thesis we use this method to show how the finite-size effects accounted by the microscopic level might significantly alter the dynamics of biological phenomena.

Life sciences.