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This book deals with theoretical and practical developments of IEF and offers detailed methodology for many of the commonly used procedures, such as IEF in gels. It is intended both as a reference guide and a practical manual.
Analytical biochemistry --- Isoelectric focusing. --- Analytical biochemistry. --- Analytic biochemistry --- Bioanalytic chemistry --- Bioanalytical chemistry --- Analytical chemistry --- Biochemistry --- Electrofocusing --- Focusing, Isoelectric --- Chemistry, Organic --- Electrophoresis --- Qualitative --- Isoelectric focusing --- 57.088.3 --- 57.08 --- 57.088.3 Methods and techniques for separation,fractionation and purification. --- Methods and techniques for separation,fractionation and purification. --- 57.08 Biological techniques. Experimental methods. Equipment --- Biological techniques. Experimental methods. Equipment
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De PCR-techniek heeft een revolutie in de moleculaire biologie ontketend en heeft tegenwoordig in verschillende laboratoria in uiteenlopende vakgebieden een vaste plaats ingenomen. Dit toegankelijke en rijk geïllustreerde boek vat alles samen wat je over de moderne PCR-praktijk moet weten. Niet alleen de fasen van de PCR-reactie, de functie van de PCR-componenten en de ontwikkeling van de PCR-primers, maar ook de nucleïnezuur-isolatie en de analyse van PCR-producten worden besproken. Daarnaast komen de real-time PCR en enkele basisbegrippen van kwaliteitszorg op het PCR-laboratorium aan de orde. Dit boek is een ideaal uitgangspunt voor studenten en laboratoriummedewerkers met geen of beperkte voorkennis van de PCR.
DNA --- PCR-Techniek --- Macromolecules --- Chemical laboratory practice --- Molecular biology --- PCR (polymerase chain reaction) --- laboratoria chemie --- DNA (deoxyribonucleic acid) --- polymeren --- moleculaire biologie --- 57.088 --- PCR --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- PCR-techniek
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De PCR-techniek heeft zich een weg gebaand naar talloze toepassingen in de medische en gerechtelijke genetica. Het betreft een manier om genetisch materiaal zo te vermeerderen en te behandelen, dat onderzoekers vrijwel ongeremd hun testen op een staal kunnen loslaten.In dit boek worden de biomedische achtergrond, het principe, de varianten en vooral de toepassingen overlopen. Het vraagt slechts een elementaire kennis van chemie. Daarom is dit werk geschikt om op hoger niveau (laboranten, bio-ingenieurs en universiteitsstudenten) de basis te verschaffen voor inzicht in de mogelijkheden van deze gentechniek.
General biochemistry --- polymers --- DNA (deoxyribonucleic acid) --- biochemie --- PCR (polymerase chain reaction) --- genetische informatie --- Molecular biology --- Analytical biochemistry --- biologie --- moleculaire biologie --- polymeren --- Macromolecules --- Erfelijkheidsleer --- Hérédité --- DNA --- 577.213 --- 57.088.2 --- 575 --- 577.1 --- 600.2 --- DNA deoxyribonucleic acid --- PCR polymerase chain reaction --- chromosomen --- erfelijkheidsleer --- gen --- genen --- genetica --- genetische manipulatie --- gentherapie --- 57.088 --- Pcr --- Genetica --- PCR --- PCR-techniek --- genetica (genen) --- Reproduction of inheritance information. Replication --- Methods and techniques for preparation, concentration and isolation. --- .chemische basis van leven. biochemie en bio-organische chemie in het algemeen --- génétique (gènes) --- Basic Sciences. Molecular Biology --- Nucleic Acids --- Nucleic Acids. --- 57.088.2 Methods and techniques for preparation, concentration and isolation. --- 577.213 Reproduction of inheritance information. Replication
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Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments.A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for e
Biological techniques --- Histology. Cytology --- Cell separation --- Cellules --- Séparation --- Cell Separation --- methods --- 57.088 --- 576.3 --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- General cytology. The cell as a biological system. General plan, chemical composition, physicochemical and molecular properties --- 576.3 General cytology. The cell as a biological system. General plan, chemical composition, physicochemical and molecular properties --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Cell separation. --- Molecular biology --- Cell isolation --- Cell segregation --- Cells --- Cytometry --- Separation (Technology) --- Technique. --- Separation --- Cell Separation - methods
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This book deals with biomimetic sensors that can quantify taste and smell - the electronic tongue and nose. Of all sensor technologies, these have been widely considered as the most difficult to realise and the development of these sensors significantly contributes to the understanding of the reception mechanisms in gustatory and olfactory systems. The author begins by dealing with the basic principles of measurement and multivariate analysis. Reception mechanisms in biological systems are briefly reviewed. Several types of biosensor, including enzyme-immobilized membranes, SPR, the quartz resonance oscillator and IC technologies are explained in detail. This book is the first to focus on artificial taste and smell sensors and also reviews conventional biosensors, such as enzyme sensors, in detail.
Biosensors. --- Chemoreceptors. --- Sensory receptors --- Chemical senses --- Biodetectors --- Biological detectors --- Biological sensors --- Biomedical detectors --- Biomedical sensors --- Detectors --- Medical instruments and apparatus --- Physiological apparatus --- Biosensors --- Chemoreceptors --- #ABIB:vcbt --- #KVIV:BB --- 543.9 --- 57.088.1 --- 664.017 --- 681.586 --- 681.586 Transducers. Sensors --- Transducers. Sensors --- 664.017 Properties of preserved foods and foodstuffs. Food quality --- Properties of preserved foods and foodstuffs. Food quality --- 57.088.1 Methods for analysis and estimation --- Methods for analysis and estimation --- 543.9 Analysis by means of biological reactions --- Analysis by means of biological reactions --- General biophysics
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Analytical biochemistry --- 543 --- 57.088 --- 577.1 --- HPLC high performance liquid chromatography --- analytische chemie (scheikunde) --- biochemie --- chromatografie --- enzymen --- kwantitatieve methoden --- laboratoriumbiochemie --- laboratoriumbiologie --- oefeningen --- radiochemie --- scheidingstechnieken --- spectroscopie --- analytische chemie (analytische scheikunde) --- .chemische basis van leven. biochemie en bio-organische chemie in het algemeen --- Problems, exercises, etc.
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Radioactive chemicals, while playing a vital role in many research and clinical techniques for nucleic acid analysis, are considered dangerous to the laboratory worker and require specialist handling and disposal equipment. This book describes how detailed molecular analysis of DNA and RNA can be performed using a range of non-radioactive chemicals giving similar if not better results than the radioactive methods. It will be a valuable book for molecular biologists and pathologists in academic and clinical research and also those working in clinical diagnostic laboratories.
57.083 --- 57.088 --- 577.2 --- DNA-onderzoek --- PCR polymerase chain reaction --- RNA ribonucleic acid --- biochemie --- biologie --- enzym- labels --- fluorescentie-labels --- hybridisatie --- immuno-labels --- immunologische technieken --- laboratoriumbiochemie --- laboratoriumbiologie --- moleculaire biologie --- probes --- Nucleic acid probes. --- Nucleic acid probes --- Oligonucleotide probes --- Probes, Nucleic acid --- Molecular probes
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Bevat alle praktische technieken samen ; zeer uitvoerige behandeling.
Biochemie --- Moleculaire biologie --- 577 --- 57.083 --- 57.088 --- analytische biochemie --- analytische chemie (scheikunde) --- centrifugeren --- chromatografie --- geïmmobiliseerde enzymen --- immunologische technieken --- immunologische testen --- in vivo activiteit --- klonen --- massaspectrometrie --- membraantransport --- rDNA recombinant DNA --- radiochemie --- receptoren --- vectoren biologie --- General biochemistry --- elektrochemie --- biochemie --- Electrochemistry --- Analytical biochemistry --- biologie --- Nuclear physics --- moleculaire biologie --- enzymen --- DNA deoxyribonucleic acid --- elektroforese --- genetica --- genetische manipulatie --- immunologie --- modellen --- proteïnen --- spectroscopie
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Chemical laboratory practice --- Analytical biochemistry --- Biochemistry --- 577.1 --- Biochemie --- 543.64 --- 57.088 --- aminozuren --- analyse --- analytische biochemie --- analytische chemie (scheikunde) --- antistoffen --- biochemie --- biologie --- biomoleculen --- centrifugeren --- chromatografie --- databases --- elektroforese --- enzymen --- isotopen --- kinetica --- koolhydraten --- laboratoriumbiochemie --- laboratoriumbiologie --- lipiden --- membranen --- metabolisme --- nucleïnezuren --- plasmiden --- proteïnen --- radiochemie --- spectrofotometrie --- transformaties --- vectoren
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Density Gradient Centrifugation
Biological techniques --- Immunology. Immunopathology --- Physical methods for diagnosis --- Clinical chemistry --- fysicochemie --- Radioimmunoassay. --- Analytical biochemistry --- Centrifugation, Density gradient --- Zonal centrifuge --- Centrifugation, Density Gradient --- 542.22 --- 57.088 --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- 542.22 Preparation of chemicals. Mechanical separation. Comminution. Pulverization. Elutriation. Spatulas. Spoons. Mortars, pestles. Mills --- Preparation of chemicals. Mechanical separation. Comminution. Pulverization. Elutriation. Spatulas. Spoons. Mortars, pestles. Mills --- Zonal ultracentrifuge --- Centrifuges --- Density gradient centrifugation --- Sedimentation analysis --- Separation (Technology) --- Centrifugations, Density Gradient --- Density Gradient Centrifugation --- Density Gradient Centrifugations --- Gradient Centrifugation, Density --- Gradient Centrifugations, Density --- Centrifugation, Density gradient. --- Zonal centrifuge. --- Centrifugation --- Centrifugation, density gradient. --- Density gradient. --- Centrifugation, Density Gradient. --- Radioimmunoassay --- Radioimmunoassays
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