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The in vivo analysis of a model organism, such as the nematode Caenorhabditis elegans, enables fundamental biomedical studies, including development, genetics, and neurobiology. In recent years, microfluidics technology has emerged as an attractive and enabling tool for the study of the multicellular organism. Advances in the application of microfluidics to C. elegans assays facilitate the manipulation of nematodes in high-throughput format and allow for the precise spatial and temporal control of their environment. In this chapter, we aim to illustrate the current microfluidic approaches for the investigation of behavior and neurobiology in C. elegans and discuss the trends of future development.

Microfluidics.

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This book focuses on the most recent technical innovations and developments in the area of ​​microfluidics, particularly in relation to their biomedical applications. The advent of microfluidics has provided important insights into the fields of biomedical research and clinical diagnosis. Special attention is given to the isolation and detection of circulating tumor cells and exosomes, the generation of concentration dilutions, the rapid sensing and detection of single molecules and viruses, and the innovations in inertial microfluidics, electrical impedance, magnetic biosensors, and droplet microfluidics.

Microfluidics. --- Fluidics --- Nanofluids

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3D printing has revolutionized the microfabrication prototyping workflow over the past few years. With the recent improvements in 3D printing technologies, highly complex microfluidic devices can be fabricated via single-step, rapid, and cost-effective protocols as a promising alternative to the time consuming, costly and sophisticated traditional cleanroom fabrication. Microfluidic devices have enabled a wide range of biochemical and clinical applications, such as cancer screening, micro-physiological system engineering, high-throughput drug testing, and point-of-care diagnostics. Using 3D printing fabrication technologies, alteration of the design features is significantly easier than traditional fabrication, enabling agile iterative design and facilitating rapid prototyping. This can make microfluidic technology more accessible to researchers in various fields and accelerates innovation in the field of microfluidics. Accordingly, this Special Issue seeks to showcase research papers, short communications, and review articles that focus on novel methodological developments in 3D printing and its use for various biochemical and biomedical applications.

Polymerization --- Cytotoxicity --- 3D printing --- Microfluidics --- Photochemistry

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Microfluidics has developed rapidly over the past three decades. Relentless diagnostic, medical and chemical applications have been demonstrated in various applications, plateforms and tools. Have microfluidics realized its full potential? Or is it only a leveraging academic tool? In this Special Issue, we focus on both insights and advancements in microfluidics. We invite emerging investigators and pioneers to contribute commentaries, perspectives and insightful reviews on related topics. The various insights from esteemed colleagues will be collated. We will also discuss technological breakthrough of original works in both short communications and full papers. The main idea is to stimulate the community and to provide an unique collection of insightful papers. We will also cover various topics ranging from 3D printing, paper-based microfludics to conventional polymer-based microfluidics which contributes to the technological advancements.

Perspective --- Insights --- Lab on a chip --- Development --- Advancement --- Microfluidics

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This book reviews the latest advancement of microfluidics and nanofluidics with a focus on electrokinetic phenomena in microfluidics and nanofluidics. It provides fundamental understanding of several new interfacial electrokinetic phenomena in microfluidics and nanofluidics. Chapter 1 gives a brief review of the fundamentals of interfacial electrokinetics. Chapter 2 shows induced charge electrokinetic transport phenomena. Chapter 3 presents the new advancement in DC dielectrophoresis. Chapter 4 introduces a novel nanofabrication method and the systematic studies of electrokinetic nanofluidics. Chapter 5 presents electrokinetic phenomena associated with Janus particles and Janus droplets. Chapter 6 introduces a new direction of electrokinetic nanofluidics: nanofluidic iontronics. Chapter 7 discusses an important differential resistive pulse sensor in microfluidics and nanofluidics.

Chemical structure --- Electrical engineering --- Biotechnology --- nanotechniek --- biotechnologie --- Electrokinetics. --- Microfluidics.. --- Nanofluids.