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Plants --- Genomics. --- Plant Physiological Phenomena. --- RNA, Plant --- Plant molecular genetics --- Plant gene expression --- Genomics --- Génétique moléculaire végétale --- Plantes --- Génomique --- genetics. --- Expression génique --- Plant gene expression. --- Plant molecular genetics. --- Genetics.

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Plant genetics --- Plants --- Genetics --- Plant gene expression --- Plant genetic regulation --- Plant gene expression. --- Plant genetic regulation. --- Plant genetics. --- Genes, Plant. --- Gene Expression Regulation, Plant. --- Genetic regulation --- Plant cellular control mechanisms --- Plant molecular genetics --- Gene expression --- Regulation, Gene Expression, Plant --- Plant Gene Expression Regulation --- Regulation of Gene Expression, Plant --- Plant Genes --- Gene, Plant --- Plant Gene --- Genome, Plant --- Regulation --- Plant Ecology

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This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model systems. This book is organized into five parts. Part I examines the tools and methods required for identifying epigenetic and conformational changes at the whole-genome level. Part II presents approaches used to determine key aspects of a gene’s function, such as techniques used to identify and characterize gene regulatory networks. This is followed by a discussion of tools used to analyze the levels of mRNA, mRNA translation rates, and metabolites. Part III features a compilation of forward and reverse genetic approaches that include recent implementation of high-throughput sequencing in classical methodologies such as QTL mapping. The final two parts explore strategies to facilitate and accelerate the generation and testing of functional DNA elements, and basic computational tools used to facilitate the use of systems biology approached by a broad spectrum of plant researchers. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and timely, Plant Functional Genomics: Methods and Protocols, Second Edition highlights the latest developments in DNA sequencing technologies that are likely to continue shaping the future of functional genomics.

Plant molecular genetics --- Plant gene expression --- Genomics --- Genome --- Eukaryota --- Gene Expression Regulation --- Organisms --- Genetic Processes --- Genetic Structures --- Genetic Phenomena --- Phenomena and Processes --- Gene Expression Regulation, Plant --- Plants --- Genome, Plant --- Botany --- Earth & Environmental Sciences --- Plant Ecology --- Plant molecular genetics. --- Plant gene expression. --- Genomics. --- Génétique moléculaire végétale --- Plantes --- Génomique --- Expression génique --- Plant Genome --- Genomes, Plant --- Plant Genomes --- Plant --- Regulation, Gene Expression, Plant --- Plant Gene Expression Regulation --- Regulation of Gene Expression, Plant --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Expression Regulation, Gene --- Regulation, Gene Action --- Regulation, Gene Expression --- Gene Action Regulation --- Regulation of Gene Expression --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Genomes --- Genome research --- Research --- Life sciences. --- Plant science. --- Botany. --- Life Sciences. --- Plant Sciences. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Biosciences --- Sciences, Life --- Science --- Floristic botany

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As more and more plant genomes are sequenced, plant researchers have been inundated with an avalanche of novel methodologies to identify the function of tens of thousands of plant genes. In Plant Functional Genomics, Erich Grotewold has assembled a team of leading plant scientists to describe in detail the most commonly used methods for investigating plant gene function in a wide variety of plants, during plant pathogen interactions, and even in algae. These readily reproducible protocols include computational, molecular, and genetic methodologies designed for both general and specific problems. Here the reader will learn how to identify genes in complex systems that have large genomes, few cells, and mixed cell systems. Readers will also learn the use of powerful computational and statistical tools to help predict gene function, either on the basis of comparative genomics, or from the analysis of complex genome sequences. Because establishing gene function relies on the identification of phenotypes, the authors expand the concept of phenotypes, including the use of multiple outputs as the ultimate phenotypic result of changes in gene activity. Numerous loss-of-function and gain-of-function techniques for discovering gene function are presented in step-by-step detail. Comprehensive and highly practical, Plant Functional Genomics offers plant biologists readily reproducible computational, molecular, and genetic protocols, powerful tools that will enable them, with little or no experience, successfully to investigate any gene function with the most recent methodologies.

577.212 --- 575.113 --- 57.088 --- 631.528.2 --- Central dogma of molecular biology. Coding of inheritance information. The genetic code and its chemical nature --- Gene. Genetic apparatus. Genome --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Genome mutations, polyploidy, haploidy --- Electronic books. -- local. --- Genomics. --- Plant gene expression. --- Plant molecular genetics. --- Plant molecular genetics --- Plant gene expression --- Genomics --- Botany --- Earth & Environmental Sciences --- Plant Ecology --- Economic sanctions. --- Security, International. --- 631.528.2 Genome mutations, polyploidy, haploidy --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- 575.113 Gene. Genetic apparatus. Genome --- 577.212 Central dogma of molecular biology. Coding of inheritance information. The genetic code and its chemical nature --- Sanctions. --- Molecular genetics --- Plant genetics --- Plant molecular biology --- Gene expression --- Genome research --- Genomes --- Research --- Sanctions économiques --- Sécurité internationale --- United Nations --- Botany. --- Plant Sciences. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Plants --- Floristic botany

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Gene Expression Regulation, Plant. --- Epigenesis, Genetic. --- Gene Silencing. --- Plant genetic regulation --- Epigenesis. --- Plantes --- Epigénèse --- Régulation génétique --- Plante --- plants --- Expression des gènes --- gene expression --- Transformation génétique --- genetic transformation --- Arn ribosomal --- ribosomal RNA --- Génie génétique --- genetic engineering --- Allélopathie --- Allelopathy --- Mutation --- mutation --- ADN recombiné --- Recombinant DNA --- Zea mays --- Arabidopsis thaliana --- Plant genetic regulation. --- Plant and Crop Sciences. Plant Breeding and Genetics --- Plant Breeding and Genetics (General) --- Plant Breeding and Genetics (General). --- Epigénèse --- Régulation génétique --- mutation. --- Epigenesis --- Plant gene expression --- Genetic regulation --- Plant cellular control mechanisms --- Plant molecular genetics --- Embryology --- Evolution (Biology) --- Genetics --- Regulation