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Staphylococcus.

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To find out if testing of up to 10 Staphylococcus aureus isolates from each sample from raw milk and raw milk products for staphylococcal enterotoxin (SE) might increase the chances of identifying potential sources of food intoxication.Methods and Results:  Altogether 386 S. aureus isolates were tested for the presence of SE by reversed passive latex agglutination (SET-RPLA), and SE genes (se) by a multiplex polymerase chain reaction (PCR). In 18 of 34 (53%) S. aureus positive samples a mixture of SE and/or se positive and negative isolates were identified. Multiplex PCR increased the number of potential SE producing strains, i.e. isolates that harboured se, with 51% among the product and 48% among the raw bovine milk isolates. Examination by pulsed-field gel electrophoresis mostly confirmed clonal similarity among isolates sharing SE/se profile, but did not further differentiate between them.Conclusions:  Isolates of S. aureus collected from one sample may show great diversity in SE production and different plating media seem to suppress or favour different strains of S. aureus.Significance and Impact of the Study:  Several isolates of S. aureus from each sample should be tested for enterotoxin production in cases with typical SE intoxication symptoms with methods that are able to reveal new SE/se.

Staphylococcus aureus.

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Methicillin resistance. --- Staphylococcus aureus infections.

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Methicillin resistance. --- Staphylococcus aureus infections. --- Staphylococcus aureus infections --- Daptomycin --- Staphylococcal Infections --- Methicillin-Resistant Staphylococcus aureus. --- Chemotherapy. --- therapeutic use. --- drug therapy.

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Staphylococcus aureus is an opportunistic human pathogen that colonizes the mucosal surfaces. It is the causative agent of various serious acute and chronic infections. Recent prevalence of infectious diseases caused and expanded by hospital-acquired methicillin-resistant S. aureus (HA-MRSA), community-acquired methicillin-resistant S. aureus (CA-MRSA) and livestock-acquired methicillin-resistant S. aureus (LA-MRSA). MRSA infections must be more serious problem in near future. It is presumed that offense and defense responses are continuously executed between S. aureus and host to establish and maintain commensalism for S. aureus. Therefore, it is essential to elucidate interplay between S. aureus and hosts to prevent staphylococcal infectious diseases. This book is comprised of content that focuses on this aspect.

Medical microbiology. --- Immunology. --- Pharmacology. --- Medical Microbiology. --- Staphylococcus aureus.