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Molecular biology --- Membranes (Biology) --- Research --- Methodology --- Membrane Lipids --- Membrane Proteins --- Cell Membrane --- Lipids --- Proteins --- Cellular Structures --- Amino Acids, Peptides, and Proteins --- Cells --- Chemicals and Drugs --- Anatomy --- Cytology --- Biology --- Health & Biological Sciences --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Cytology. --- Membranes (Biology) - Research - Methodology

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As the technology base for the preparation of increasingly c- plex peptides has improved, the methods for their purification and ana- sis have also been improved and supplemented. Peptide science routinely utilizes tools and techniques that are common to organic chemistry, p- tein chemistry, biophysical chemistry, enzymology, pharmacology, and molecular biology. A fundamental understanding of each of these areas is essential for interpreting all of the data that a peptide scientist may see. The purpose of Peptide Analysis Protocols is to provide the novice with sufficient practical information necessary to begin developing useful analysis and separation skills. Understanding and developing these skills will ultimately yield a scientist with broadened knowledge and good problem-solving abilities. Although numerous books that address d- ferent specialties, such as HPLC, FAB-MS, CE, and NMR, have been written, until now no single volume has reviewed all of these techniques with a focus on "getting started" in separation and analysis of peptides. This volume will also provide those who already possess practical knowledge of the more advanced aspects of peptide science with detailed applications for each of these protocols. Because the chapters have been written by researchers active in each of the fields that they discuss, a great deal of information on and insight into solution of real problems that they have encountered is presented. Examplary results are clearly demonstrated and discussed. For more advanced investi- tions, supplementary experiments are often suggested.

Peptides --- Analysis --- Laboratory manuals --- analysis --- laboratory manuals --- Peptide --- peptides --- Technique analytique --- Analytical methods --- HPLC --- Chromatographie --- Chromatography --- Électrophorèse --- Electrophoresis --- Spectrométrie --- Spectrometry --- methods --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Laboratory manuals. --- Biochemistry. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Organic compounds --- Amino acids --- laboratory manuals. --- Biochemistry, general.

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Although the complement system-a vital part of the body's defense against bacteria-was discovered more than a century ago, its study has undergone a renaissance with the identification of its regulatory molecules and the realization that these molecules can be used therapeutically. In Complement Methods and Protocols, B. Paul Morgan and a team of expert laboratorians present a comprehensive set of readily reproducible methods to study this critical system. These cutting-edge techniques are suitable both for the basic scientist interested in understanding complement's mechanisms of activation and for the clinical scientist wishing to quantify its activation, and range from the purification of its components to generating complement-deficient mice by gene deletion. Additional techniques presented include procedures for the analysis of complement function, for the study of its regulators, for detection of its activation in vivo, and for the identification of its autoantibodies. Comprehensive and cutting-edge, Complement Methods and Protocols offers today's basic and clinical investigators powerful tools for the analysis of the role of complement in human pathophysiology and disease, as well as its therapeutic regulation.

Complement (Immunology) -- Laboratory manuals. --- Electronic books. -- local. --- Immunology. --- Complement (Immunology) --- Immunoproteins --- Blood Proteins --- Proteins --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Complement System Proteins --- Biology --- Health & Biological Sciences --- Microbiology & Immunology --- Immunobiology --- Life sciences --- Serology --- Alexin --- Complements (Immunity) --- Blood proteins --- Complement system proteins --- analysis

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In Adhesion Protein Protocols, an international corps of expert investigators describe their optimized techniques for both the identification of new cell adhesion proteins and the characterization of novel adhesive structures. Their cutting edge methods will enable both novice and experienced researchers readily to identify and clone new adhesion proteins, to successfully produce inhibitors of adhesive activity, to develop biological models for the assay of cell-to-matrix and cell-to-cell adhesion in vitro, and to measure the signaling activities of adhesion proteins. The protocols described here constitute a guide to some of the most important assays in cell biology, promising significant results not only to those interested in adhesion processes, but also to scientists from many different disciplines. Adhesion Protein Protocols is the first collection of methodologies devoted entirely to the study of adhesion molecules. By illuminating these molecules and the possibilities for manipulating them, the new experimental strategies collected here offer powerful approaches to the regulation of immunity, inflammation, tissue remodeling, and embryonic development. The book will become the standard source for those wishing to discover new adhesion molecules and to characterize their biological activity.

Cell adhesion molecules --- Membrane Glycoproteins --- Antigens, Surface --- Glycoproteins --- Membrane Proteins --- Antigens --- Biological Factors --- Proteins --- Chemicals and Drugs --- Amino Acids, Peptides, and Proteins --- Cell Adhesion Molecules --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Laboratory manuals --- Cell Adhesion Molecules. --- Laboratory manuals. --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Cytology.

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It is now clear that neuropeptide Y (NPY), the most abundant peptide present in the mammalian nervous system, is implicated in the pathophysiology of many diseases, including eating disorders, seizures, memory loss, anxiety, depression, and cardiovascular diseases, and offers major therapeutic opportunities for their treatment. In Neuropeptide Y Protocols, a strong cohort of established investigators from a variety of disciplines detail their best techniques for advancing NPY research. Among the many readily reproducible methods described are those for cloning, for the production of transgenic and knockout models, and for the use of antisense technology to define the receptors and the signal transduction pathways mediating NPY actions. A range of the major qualitative and quantitative techniques used to study tissue mRNA distribution are also included, as well as methods for radioligand binding. The cloning techniques utilize genomic DNA isolation, expression cloning, PCR cloning, the construction of hybrid receptors, and homology-based cloning. Highly practical and state-of-the-art, Neuropeptide Y Protocols constitutes a gold-standard collection of proven techniques for advancing today's understanding of this significant neuropeptide, as well as for developing the promising NPY-based therapeutics now emerging for many important disorders.

Electronic books. -- local. --- Genetics -- Laboratory manuals. --- Neuropeptide Y -- Laboratory manuals. --- Neuropeptide Y --- Neuropeptides --- Peptides --- Nerve Tissue Proteins --- Amino Acids, Peptides, and Proteins --- Proteins --- Chemicals and Drugs --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Genetics --- Neuropeptide tyrosine --- NPY (Neuropeptide) --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Neurosciences. --- Neural sciences --- Neurological sciences --- Neuroscience --- Medical sciences --- Nervous system