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Because cytokines regulate many different bodily functions in living organisms, mainly at the level of cell-cell communications, there is great interest in understanding their mode of action. In Cytokine Protocols, established researchers, physicians, and clinicians present their best biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. Described in step-by-step detail to ensure successful experimental results, these protocols cover the large-scale generation and purification of plasmid DNA, the identification of DNA-protein interactions via the gel mobility shift assay, RNA-level phenomena, and the isolation and characterization of cytokines, cytokine-related proteins, and their interactions. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization. Each protocol includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding pitfalls, and, where needed, a discussion of the interpretation of results. Comprehensive and highly practical, Cytokine Protocols offers novice researchers a concise introduction to the principles of cytokine research, and experienced investigators all the advanced techniques and time-saving tips needed to ensure productive research.

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Immunologic Techniques. --- Antibody Dissociation --- Immunologic Technic --- Immunologic Technics --- Immunologic Technique --- Immunological Technics --- Immunological Techniques --- Technic, Immunologic --- Technics, Immunologic --- Technique, Immunologic --- Techniques, Immunologic --- Antibody Dissociations --- Dissociation, Antibody --- Dissociations, Antibody --- Immunological Technic --- Immunological Technique --- Technic, Immunological --- Technics, Immunological --- Technique, Immunological --- Techniques, Immunological --- Allergy and Immunology --- Immunity --- Immunochemistry --- methods --- Immunodiagnosis --- Immunology --- Handbooks, manuals, etc. --- Immunologic Techniques --- Immunobiology --- Life sciences --- Serology --- Immunological diagnosis --- Clinical immunology --- Diagnosis, Laboratory --- Immunoassay --- Handbooks, manuals, etc

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Allergy and Immunology --- Immunotherapy, Active --- HLA-D Antigens --- Immunologic Techniques --- Immunologic Tests --- Receptors, Immunologic --- Immunodiagnosis --- Diagnostics immunologiques --- Periodicals. --- Périodiques --- Immunodiagnosis. --- Allergy and Immunology. --- Immunotherapy, Active. --- HLA-D Antigens. --- Immunologic Techniques. --- Immunologic Tests. --- Receptors, Immunologic. --- Science --- Immunologic Receptor --- Immunological Receptors --- Immunologic Receptors --- Receptor, Immunologic --- Receptors, Immunological --- Diagnosis, Immunologic --- Immunologic Diagnosis --- Immunologic Test --- Immunological Tests --- Tests, Immunologic --- Diagnosis, Immunological --- Diagnoses, Immunologic --- Diagnoses, Immunological --- Immunodiagnoses --- Immunologic Diagnoses --- Immunological Diagnoses --- Immunological Diagnosis --- Immunological Test --- Test, Immunologic --- Test, Immunological --- Tests, Immunological --- Antibody Dissociation --- Immunologic Technic --- Immunologic Technics --- Immunologic Technique --- Immunological Technics --- Immunological Techniques --- Technic, Immunologic --- Technics, Immunologic --- Technique, Immunologic --- Techniques, Immunologic --- Antibody Dissociations --- Dissociation, Antibody --- Dissociations, Antibody --- Immunological Technic --- Immunological Technique --- Technic, Immunological --- Technics, Immunological --- Technique, Immunological --- Techniques, Immunological --- HLA-D --- HLA-Dw --- Immune Response Associated Antigens, Human --- Antigens, HLA-D --- Class II Human Antigens --- HLA-Dw Antigens --- Human Class II Antigens --- Ia-Like Antigens, Human --- Immune Response-Associated Antigens, Human --- Immune-Associated Antigens, Human --- Immune-Response Antigens, Human --- Antigens, HLA D --- Antigens, HLA-Dw --- Antigens, Human Ia-Like --- Antigens, Human Immune-Associated --- Antigens, Human Immune-Response --- HLA D Antigens --- HLA Dw Antigens --- Human Ia-Like Antigens --- Human Immune-Associated Antigens --- Human Immune-Response Antigens --- Ia Like Antigens, Human --- Immune Associated Antigens, Human --- Immune Response Antigens, Human --- Active Immunotherapy --- Therapy, Vaccine --- Immune RNA Manipulation --- Vaccine Therapy --- Active Immunotherapies --- Immune RNA Manipulations --- Immunotherapies, Active --- Manipulation, Immune RNA --- Manipulations, Immune RNA --- RNA Manipulation, Immune --- RNA Manipulations, Immune --- Therapies, Vaccine --- Vaccine Therapies --- Allergy Specialty --- Allergy, Immunology --- Immunology and Allergy --- Immunology, Allergy --- Immunology --- Specialty, Allergy --- Immunological diagnosis --- methods --- Immunity --- Immunochemistry --- Clinical immunology --- Diagnosis, Laboratory --- Immunoassay

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Immunodiagnosis --- Allergy and Immunology. --- HLA-D Antigens. --- Immunologic Techniques. --- Immunologic Tests. --- Receptors, Immunologic. --- Diagnostics immunologiques --- Immunodiagnosis. --- Immunological diagnosis --- Immunologic Receptor --- Immunological Receptors --- Immunologic Receptors --- Receptor, Immunologic --- Receptors, Immunological --- Diagnosis, Immunologic --- Immunologic Diagnosis --- Immunologic Test --- Immunological Tests --- Tests, Immunologic --- Diagnosis, Immunological --- Diagnoses, Immunologic --- Diagnoses, Immunological --- Immunodiagnoses --- Immunologic Diagnoses --- Immunological Diagnoses --- Immunological Diagnosis --- Immunological Test --- Test, Immunologic --- Test, Immunological --- Tests, Immunological --- Antibody Dissociation --- Immunologic Technic --- Immunologic Technics --- Immunologic Technique --- Immunological Technics --- Immunological Techniques --- Technic, Immunologic --- Technics, Immunologic --- Technique, Immunologic --- Techniques, Immunologic --- Antibody Dissociations --- Dissociation, Antibody --- Dissociations, Antibody --- Immunological Technic --- Immunological Technique --- Technic, Immunological --- Technics, Immunological --- Technique, Immunological --- Techniques, Immunological --- Allergy and Immunology --- HLA-D --- HLA-Dw --- Immune Response Associated Antigens, Human --- Antigens, HLA-D --- Class II Human Antigens --- HLA-Dw Antigens --- Human Class II Antigens --- Ia-Like Antigens, Human --- Immune Response-Associated Antigens, Human --- Immune-Associated Antigens, Human --- Immune-Response Antigens, Human --- Antigens, HLA D --- Antigens, HLA-Dw --- Antigens, Human Ia-Like --- Antigens, Human Immune-Associated --- Antigens, Human Immune-Response --- HLA D Antigens --- HLA Dw Antigens --- Human Ia-Like Antigens --- Human Immune-Associated Antigens --- Human Immune-Response Antigens --- Ia Like Antigens, Human --- Immune Associated Antigens, Human --- Immune Response Antigens, Human --- Allergy Specialty --- Allergy, Immunology --- Immunology and Allergy --- Immunology, Allergy --- Immunology --- Specialty, Allergy --- methods --- HLA-D Antigens --- Immunologic Techniques --- Immunologic Tests --- Receptors, Immunologic --- Immunity --- Immunochemistry --- Clinical immunology --- Diagnosis, Laboratory --- Immunoassay --- Journal --- Periodicals --- HLA-D Antigens.. --- HLA histocompatibility antigens. --- Antigènes HLA-D. --- Diagnostics immunologiques. --- HL-A histocompatibility antigens --- HLA antigens --- HLA transplantation antigens --- Human leukocyte antigens --- Transplantation antigens, Human --- Histocompatibility antigens --- Major histocompatibility complex