Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This volume aims at providing readers with protocols to enhance their research for the next decade of phytochrome research. The chapters in this book cover topics such as tests for light-dependent interaction of phytochrome binding proteins using yeast two hybrid assays, in vitro pull down, and co-immunoprecipitation from stable transgenic lines; investigations into phosphorylation, SUMOylation, and ubiquitination of phytochromes and other proteins; a look at how in vivo spectroscopy can be used to measure Pfr levels of phytochrome in planta; identifying hormonal action and co-action in light-regulated processes; and ways to use the CRISPR/Cas9 system to generate Physcomitrella mutant lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Phytochromes: Methods and Protocols serves as a guideline to researchers who are new to the field, and a stepping stone for experienced researchers looking to expand their knowledge of this evolving field. .

Botany. --- Plant Sciences. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Plants --- Floristic botany --- Pigments vegetals --- Manuals de laboratori --- Protocols clínics --- Plans d'actuació mèdica --- Protocols en medicina --- Protocols de diagnòstic clínic --- Protocols mèdics --- Protocols terapèutics --- Medicina clínica --- Històries clíniques --- Protocols d'infermeria --- Tècniques de laboratori --- Fitoquímica --- Flavonoides --- Melanina --- Color de les plantes

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Amino acids --- Amino Acids. --- Amino acids. --- Acids, Amino --- Amino compounds --- Organic acids --- Peptides --- aminozuren --- Biochemistry --- Amino Acid --- Acid, Amino --- Bioquímica. --- Aminoàcids. --- Àcids aminats --- Àcid glutàmic --- Aminoàcids essencials --- Aminoàcids sulfurosos --- Cisteïna --- Glutamina --- Protamines --- Química biològica --- Química fisiològica --- Biologia --- Medicina --- Química --- Absorció (Fisiologia) --- Anàlisi enzimàtica --- Biodegradació --- Biodisponibilitat --- Bioelectroquímica --- Bioenergètica --- Biogeoquímica --- Biologia molecular --- Bioquímica analítica --- Bioquímica clínica --- Bioquímica física --- Bioquímica quàntica --- Biosíntesi --- Centres actius (Bioquímica) --- Citoquímica --- Composició del cos humà --- Cromatografia --- Digestió --- Enginyeria bioquímica --- Enzimologia --- Equilibri àcid-base --- Fitoquímica --- Fixació de proteïnes --- Genètica bioquímica --- Immunoquímica --- Interfícies biològiques --- Lípids --- Lligands (Bioquímica) --- Marcadors bioquímics --- Metabolisme --- Minerals en l'organisme --- Neuroquímica --- Oxidació fisiològica --- Pigments (Biologia) --- Química biorgànica --- Química clínica --- Química microbiològica --- Quimiotaxi --- Quimiotaxonomia --- Toxicologia bioquímica --- Xenobiòtics --- Histoquímica

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Clinical biochemistry --- Biochemistry. --- Chemistry, Clinical. --- Bioquímica clínica --- Bioquímica --- Química clínica --- Clinical biochemistry. --- Revistes. --- Clinical Chemistry --- Medical biochemistry --- Pathobiochemistry --- Pathological biochemistry --- Biochemistry --- Chemistry, Clinical --- Bioquímica. --- Bioquímica clínica. --- Química clínica. --- Periodicals --- Revistes --- fast --- (OCoLC)fst00864329 --- Pathology --- Clinical Chemistry Tests --- Chemistry, Analytic --- Anàlisis clíniques --- Diagnòstic bioquímic --- Diagnòstic químic --- Química mèdica --- Diagnòstic de laboratori --- Anàlisi d'orina --- Anàlisi de sang --- Enzimologia clínica --- Valors de referència (Medicina) --- Laboratoris clínics --- Bioquímica de les malalties --- Bioquímica mèdica --- Bioquímica patològica --- Patobioquímica --- Bioquímica clínica veterinària --- Química biològica --- Química fisiològica --- Biologia --- Medicina --- Química --- Absorció (Fisiologia) --- Anàlisi enzimàtica --- Biodegradació --- Biodisponibilitat --- Bioelectroquímica --- Bioenergètica --- Biogeoquímica --- Biologia molecular --- Bioquímica analítica --- Bioquímica física --- Bioquímica quàntica --- Biosíntesi --- Centres actius (Bioquímica) --- Citoquímica --- Composició del cos humà --- Cromatografia --- Digestió --- Enginyeria bioquímica --- Enzimologia --- Equilibri àcid-base --- Fitoquímica --- Fixació de proteïnes --- Genètica bioquímica --- Immunoquímica --- Interfícies biològiques --- Lípids --- Lligands (Bioquímica) --- Marcadors bioquímics --- Metabolisme --- Minerals en l'organisme --- Neuroquímica --- Oxidació fisiològica --- Pigments (Biologia) --- Química biorgànica --- Química microbiològica --- Quimiotaxi --- Quimiotaxonomia --- Toxicologia bioquímica --- Xenobiòtics --- Histoquímica

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Expanding upon the research elucidated by the first volume of this collection, Advanced Protocols in Oxidative Stress II presents thirty additional cutting-edge chapters focusing on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data. The international panel of authors also provide animal models and numerous studies concentrating on mitochondria during hypoxic conditions using advanced methods for pO2, peroxynitrate, reactive S-nitrosothiols, lipid peroxides, COX, and the mitochondrial membrane potential. Due to the dynamic nature of this topic, this book is the second of several volumes of Advanced Protocols in Oxidative Stress, all included in the highly successful Methods in Molecular Biology™ series. As part of the series, the chapters of this volume present brief introductions to the respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting to ensure easy replication of the technology involved. Authoritative and convenient, Advanced Protocols in Oxidative Stress II is an ideal desk reference for scientists wishing to further the research in this exciting, unique, and vital field of study.

Biochemistry. --- Cytology. --- Analytical biochemistry. --- Biochemistry, general. --- Cell Biology. --- Analytical Chemistry. --- Oxidative Stress. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Analytic biochemistry --- Biochemistry --- Chemistry, Analytic --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Composition --- Bioanalytic chemistry --- Bioanalytical chemistry --- Analytical chemistry --- Estrès oxidatiu --- Bioquímica --- Manuals de laboratori --- Química biològica --- Química fisiològica --- Biologia --- Medicina --- Química --- Absorció (Fisiologia) --- Anàlisi enzimàtica --- Biodegradació --- Biodisponibilitat --- Bioelectroquímica --- Bioenergètica --- Biogeoquímica --- Biologia molecular --- Bioquímica analítica --- Bioquímica clínica --- Bioquímica física --- Bioquímica quàntica --- Biosíntesi --- Centres actius (Bioquímica) --- Citoquímica --- Composició del cos humà --- Cromatografia --- Digestió --- Enginyeria bioquímica --- Enzimologia --- Equilibri àcid-base --- Fitoquímica --- Fixació de proteïnes --- Genètica bioquímica --- Immunoquímica --- Interfícies biològiques --- Lípids --- Lligands (Bioquímica) --- Marcadors bioquímics --- Metabolisme --- Minerals en l'organisme --- Neuroquímica --- Oxidació fisiològica --- Pigments (Biologia) --- Química biorgànica --- Química clínica --- Química microbiològica --- Quimiotaxi --- Quimiotaxonomia --- Toxicologia bioquímica --- Xenobiòtics --- Histoquímica --- Estrès (Fisiologia) --- Reacció d'oxidació-reducció --- Tècniques de laboratori

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This third edition volume expands on the previous editions with many new chapters that cover the latest techniques and topics that were not addressed in the previous volumes. The chapters in this book are divided into three parts: Part One covers in vitro assays that focus on a variety of amyloids and how to study these peptides and proteins. Part Two describes cell culture models and assays, and Part Three explores methods on how to extract amyloid from tissue, its detection, and its characterization in vitro or in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Amyloid Proteins: Methods and Protocols, Third Edition is a valuable resource for both students and scientists who are new to the field, as well as experienced researchers who would like to progress their research with the latest available techniques.

Biochemistry. --- Protein Science. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Amiloides --- Proteïnes --- Bioquímica --- Manuals de laboratori --- Química biològica --- Química fisiològica --- Biologia --- Medicina --- Química --- Absorció (Fisiologia) --- Anàlisi enzimàtica --- Biodegradació --- Biodisponibilitat --- Bioelectroquímica --- Bioenergètica --- Biogeoquímica --- Biologia molecular --- Bioquímica analítica --- Bioquímica clínica --- Bioquímica física --- Bioquímica quàntica --- Biosíntesi --- Centres actius (Bioquímica) --- Citoquímica --- Composició del cos humà --- Cromatografia --- Digestió --- Enginyeria bioquímica --- Enzimologia --- Equilibri àcid-base --- Fitoquímica --- Fixació de proteïnes --- Genètica bioquímica --- Immunoquímica --- Interfícies biològiques --- Lípids --- Lligands (Bioquímica) --- Marcadors bioquímics --- Metabolisme --- Minerals en l'organisme --- Neuroquímica --- Oxidació fisiològica --- Pigments (Biologia) --- Química biorgànica --- Química clínica --- Química microbiològica --- Quimiotaxi --- Quimiotaxonomia --- Toxicologia bioquímica --- Xenobiòtics --- Histoquímica --- Biomolècules --- Albúmines --- Apoproteïnes --- Conformació de proteïnes --- Enzims --- Factors de transcripció --- Glicoproteïnes --- Lipoproteïnes --- Nucleoproteïnes --- Pèptids --- Prions --- Protamines --- Proteïnes citosquelètiques --- Proteïnes de membrana --- Proteïnes de la sang --- Proteïnes recombinants --- Proteïnes supressores de tumors --- Receptors cel·lulars --- Ubiqüitina --- Xaperones moleculars --- Proteòmica --- Tècniques de laboratori --- Amiloide --- Substància amiloide --- Amiloïdosi