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The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years.  In cDNA Libraries: Methods and Protocols, expert researchers provide current techniques that reflect the latest advances in the construction and application of cDNA libraries.  The first half of the volume covers improved approaches to some of the most basic elements of creating cDNA libraries, while the second half casts a much wider net and includes visionary applications of cDNA technology which were either unforeseen or technically impractical until recently.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, cDNA Libraries: Methods and Protocols serves as an ideal guide to all scientists seeking to advance this important technology and provide answers to the enduring fundamental questions of biology.

Antisense DNA --- Publication Formats --- Genetic Structures --- Genetic Phenomena --- Publication Characteristics --- Phenomena and Processes --- Laboratory Manuals --- Gene Library --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- DNA, Complementary --- Antisense nucleic acids --- Acides nucléiques antisens --- DNA Library --- cDNA Library --- DNA Libraries --- Gene Libraries --- Libraries, DNA --- Libraries, Gene --- Libraries, cDNA --- Library, DNA --- Library, Gene --- Library, cDNA --- cDNA Libraries --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Complementary DNA --- DNA, Complementary. --- Human genetics. --- Gene expression. --- Human Genetics. --- Gene Expression. --- Genes --- Genetic regulation --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Expression

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This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model systems. This book is organized into five parts. Part I examines the tools and methods required for identifying epigenetic and conformational changes at the whole-genome level. Part II presents approaches used to determine key aspects of a gene’s function, such as techniques used to identify and characterize gene regulatory networks. This is followed by a discussion of tools used to analyze the levels of mRNA, mRNA translation rates, and metabolites. Part III features a compilation of forward and reverse genetic approaches that include recent implementation of high-throughput sequencing in classical methodologies such as QTL mapping. The final two parts explore strategies to facilitate and accelerate the generation and testing of functional DNA elements, and basic computational tools used to facilitate the use of systems biology approached by a broad spectrum of plant researchers. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and timely, Plant Functional Genomics: Methods and Protocols, Second Edition highlights the latest developments in DNA sequencing technologies that are likely to continue shaping the future of functional genomics.

Plant molecular genetics --- Plant gene expression --- Genomics --- Genome --- Eukaryota --- Gene Expression Regulation --- Organisms --- Genetic Processes --- Genetic Structures --- Genetic Phenomena --- Phenomena and Processes --- Gene Expression Regulation, Plant --- Plants --- Genome, Plant --- Botany --- Earth & Environmental Sciences --- Plant Ecology --- Plant molecular genetics. --- Plant gene expression. --- Genomics. --- Génétique moléculaire végétale --- Plantes --- Génomique --- Expression génique --- Plant Genome --- Genomes, Plant --- Plant Genomes --- Plant --- Regulation, Gene Expression, Plant --- Plant Gene Expression Regulation --- Regulation of Gene Expression, Plant --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Expression Regulation, Gene --- Regulation, Gene Action --- Regulation, Gene Expression --- Gene Action Regulation --- Regulation of Gene Expression --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Genomes --- Genome research --- Research --- Life sciences. --- Plant science. --- Botany. --- Life Sciences. --- Plant Sciences. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Biosciences --- Sciences, Life --- Science --- Floristic botany

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Genetics --- Biochemistry --- Biochemical genetics --- Génétique biochimique --- periodicals --- Periodicals --- Périodiques --- Biochemistry. --- Genetics. --- Biochemical genetics. --- Chemistry --- General and Others --- Computational Biosciences --- Health Sciences --- Information Technology --- Life Sciences --- Biology --- Chemistry. --- General and Others. --- Health Sciences. --- Information Technology. --- Life Sciences. --- Molecular Biology. --- Biochemical Genetics --- Biology, Molecular --- Genetics, Biochemical --- Genetics, Molecular --- Molecular Genetics --- Biochemical Genetic --- Genetic, Biochemical --- Genetic, Molecular --- Molecular Genetic --- Chemical genetics --- Genetic Phenomena --- Molecular genetics --- Genetic Structures --- Molecular biology. --- biochemistry. --- genetics. --- molecular biology. --- Biological Sciences --- bioinformatics --- molecular dynamics --- molecular epidemiology --- molecular genetics --- molecular models --- molecular systematics --- animal genetics --- cytogenetics --- gender --- genetic equilibrium --- genetic factors --- genetic relationships --- genetic selection --- genetic stability --- genetic structure --- genetic traits --- genetic vectors --- genomics --- human genetics --- immunogenetics --- inheritance (genetics) --- landscape genetics --- maternal effect --- metabolomics --- microbial genetics --- nutrigenomics --- paternal effect --- phenomics --- phenotype --- plant genetics --- population genetics --- proteomics --- quantitative genetics --- reproductive fitness --- sex determination --- transcriptomics --- biotechnology --- breeding --- genetic models --- genetic resources --- geneticists --- bioaccumulation --- bioactive properties --- bioavailability --- biochemical compounds --- biochemical mechanisms --- biochemical pathways --- biochemical polymorphism --- bioluminescence --- biomineralization --- biotransformation --- endogenous sources --- enzymatic reactions --- enzymology --- exogenous sources --- glycomics --- inactivation --- metabolism --- synergism --- physiology --- plant biochemistry --- serology --- Biochimie. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Medical sciences --- Composition --- Genètica bioquímica

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In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this first volume of Gene Therapy Protocols: Production and In Vivo Applications of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering. This first volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.

Gene therapy --- Gene Transfer Techniques --- Genetic Vectors --- Genetic Techniques --- Genetic Structures --- Genetic Phenomena --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Phenomena and Processes --- Pathology --- Medicine --- Health & Biological Sciences --- Methodology --- Genetic Therapy --- Thérapie génique --- methods. --- Methodology. --- Méthodologie --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Cloning Vectors --- Shuttle Vectors --- Vectors, Genetic --- Cloning Vector --- Genetic Vector --- Shuttle Vector --- Vector, Cloning --- Vector, Genetic --- Vector, Shuttle --- Vectors, Cloning --- Vectors, Shuttle --- Gene Delivery Systems --- Gene Transfer Technique --- Transgenesis --- Delivery System, Gene --- Delivery Systems, Gene --- Gene Delivery System --- Technique, Gene Transfer --- Techniques, Gene Transfer --- Transfer Technique, Gene --- Transfer Techniques, Gene --- Therapy, Gene --- methods --- Human genetics. --- Animal genetics. --- Gene therapy. --- Human Genetics. --- Animal Genetics and Genomics. --- Gene Therapy. --- Genetic engineering --- Therapeutics --- Heredity, Human --- Human biology --- Physical anthropology

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Vision --- Eye --- Oeil --- Genetic aspects --- Periodicals --- Cytology --- Molecular aspects --- Aspect génétique --- Périodiques --- Cytologie --- Aspect moléculaire --- Cells. --- Molecular Biology. --- Vision. --- Œil --- genetics. --- Eyesight --- Seeing --- Sight --- Light Signal Transduction, Visual --- Ocular Vision --- Visual Light Signal Transduction --- Visual Phototransduction --- Visual Transduction --- Phototransduction, Visual --- Transduction, Visual --- Biochemical Genetics --- Biology, Molecular --- Genetics, Biochemical --- Genetics, Molecular --- Molecular Genetics --- Biochemical Genetic --- Genetic, Biochemical --- Genetic, Molecular --- Molecular Genetic --- Cell --- Eyeball --- Eyes --- Visual system --- Senses and sensation --- Blindfolds --- Physiological optics --- Ophthalmology --- Visual Perception --- Genetic Phenomena --- Cell Biology --- Face --- Photoreceptors --- Vision, Ocular. --- Vision, Ocular --- Aspect moléculaire --- Aspect génétique --- Cytology. --- molecular biology --- ophthalmology --- genetics --- biochemistry --- cell biology --- Cells --- Molecular Biology --- cells. --- molecular biology. --- vision. --- genetics --- Biological Sciences --- animal genetics --- cytogenetics --- gender --- genetic equilibrium --- genetic factors --- genetic relationships --- genetic selection --- genetic stability --- genetic structure --- genetic traits --- genetic vectors --- genomics --- human genetics --- immunogenetics --- inheritance (genetics) --- landscape genetics --- maternal effect --- metabolomics --- microbial genetics --- molecular genetics --- nutrigenomics --- paternal effect --- phenomics --- phenotype --- plant genetics --- population genetics --- proteomics --- quantitative genetics --- reproductive fitness --- sex determination --- transcriptomics --- biotechnology --- breeding --- genetic models --- genetic resources --- geneticists --- eyesight --- visual phototransduction --- visual transduction --- ocular physiology --- sensation --- color vision --- night vision --- eye diseases --- ophthalmology --- photoreceptors --- sensory properties --- vision disorders --- visual cortex --- visual perception --- bioinformatics --- molecular dynamics --- molecular epidemiology --- molecular models --- molecular systematics --- cell biology --- acinar cells --- adipocytes --- aleurone cells --- antigen-presenting cells --- bacteriocytes --- blood cells --- bone marrow cells --- bundle sheath cells --- chondrocytes --- chromaffin cells --- chromatophores --- coelomocytes --- cultured cells --- endothelial cells --- epithelial cells --- eukaryotic cells --- fibroblasts --- germ cells --- giant cells --- gonadotropic cells --- granulosa cells --- guard cells --- hemocytes --- hepatocytes --- islets of Langerhans --- keratinocytes --- kidney cells --- Leydig cells --- luteal cells --- mast cells --- myocytes --- neoplasm cells --- neurosecretory cells --- nurse cells --- osteoblasts --- osteoclasts --- phagocytes --- plant root cells --- prokaryotic cells --- Schwann cells --- Sertoli cells --- somatic cells --- splenocytes --- spores --- stem cells --- stromal cells --- tenocytes --- teratocytes --- thecal cells --- vegetative cells --- artificial cells --- cell transplantation --- cell viability --- cellular microenvironment --- Cellules. --- sight (sense) --- Organisms --- Biologia molecular. --- Visió.