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Molecular biology --- Genetics --- Biochemistry --- Genetic Techniques. --- Molecular Biology --- Methodology --- Technique --- methods. --- Biophysics
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Gene mapping --- Biotechnology --- Genetics --- Sequence alignment (Bioinformatics) --- Genetic Techniques --- Sequence Alignment --- Sequence Analysis --- Cartes chromosomiques --- Biotechnologie --- Génétique --- Homologie de séquence (Bio-informatique) --- Technique --- Technique.
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This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Drosophila: Methods and Protocols, Third Edition serves as a useful and practical guide to new researchers and experts using Drosophila as a model system. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Biological models. --- Biology—Technique. --- Genetics. --- Biological Models. --- Genetic Techniques. --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Models, Biological
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Genetics --- Genetic Techniques --- Génétique --- Periodicals --- Technique --- Périodiques --- Genetic Techniques. --- Genetics. --- Genetics & Genomics. --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- methods --- genetics --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Genetic Structures --- Genetic Phenomena --- Genetic Counseling
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Génomes. --- Bio-informatique. --- Génomique --- Génétique. --- Méthodologie. --- Genome --- Genomics --- Gene Expression --- Polymorphism, Single Nucleotide --- genetics --- Genetica --- Génétique --- Génomes. --- Génomique --- Génétique. --- Méthodologie. --- Genetic Techniques. --- Polymorphism, Single Nucleotide - genetics --- Genetic Techniques --- Genomes. --- Genome Components. --- Molecular Probe Techniques. --- Génome. --- Composants de génome. --- Techniques de sonde moléculaire. --- Techniques génétiques.
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In Lipoprotein Protocols, Jose Ordovas compiles a cutting-edge collection of molecular protein techniques for studying advanced aspects of lipoprotein structure and metabolism. These step-by-step methods allow both the beginning and the advanced researcher to carry out not only fundamental, but also the most advanced genetic screening techniques, including RT-PCR and the preparation of relevant models of transgenic and knockout mice. In addition, novel techniques are presented to characterize plasma lipoproteins, their subfractions, and their modifications, and to determine the activity of relevant enzymes and carrier proteins. Each method is described in easily reproducible detail by hands-on experts who have perfected it. Lipoprotein Protocols offers many powerful tools for unraveling the complexity of lipoprotein metabolism and determining the individual risk of coronary heart disease. For all those basic and clinical investigators seeking to advance the frontier of lipoprotein research and develop novel genetic therapies for the treatment of cardiovascular disease, this eminently useful book constitutes the new standard reference and guide.
Lipoproteins --- Genetic Techniques. --- Microbiological Techniques. --- Analysis. --- analysis --- Microbiological Techniques --- Laboratory Manuals --- Genetic Techniques --- Publication Formats --- Proteins --- Lipids --- Investigative Techniques --- Clinical Laboratory Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemicals and Drugs --- Amino Acids, Peptides, and Proteins --- Publication Characteristics --- Physiology --- Human Anatomy & Physiology --- Health & Biological Sciences --- Analysis --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Lipoproteins - Analysis. --- Lipoproteins - analysis - laboratory manuals.
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This book combines discursive chapters that present the latest progress in molecular biology, drug discovery, organ-tissue engineering, and related fields, with a number of descriptive chapters on methods, protocols, and case studies. Structured into four parts, this volume walks the reader through the latest in cellular biology, with discussions on novel medicinal plant metabolites, nanotechnology in precision medicine, nucleic acid-based therapeutics and vaccines, genetic engineering, computational aid, bioinformatics, synthetic organs for transplantation, and organ-tissue engineering. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that ensures quality results in the lab. Authoritative and informative, Gene, Drug, and Tissue Engineering serves as an ideal guide for undergraduate students, postgraduate researchers, and senior researchers working in biomedicine and its underlying technologies, stimulating both computational and experimental development and fostering the exchange of new ideas.
Biology—Technique. --- Genetics. --- Regenerative medicine. --- Pharmaceutical chemistry. --- Genetic Techniques. --- Regenerative Medicine and Tissue Engineering. --- Pharmaceutics. --- Chemistry, Medical and pharmaceutical --- Chemistry, Pharmaceutical --- Drug chemistry --- Drugs --- Medical chemistry --- Medicinal chemistry --- Pharmacochemistry --- Chemistry --- Medicine --- Regeneration (Biology) --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Tissue Engineering. --- Regenerative Medicine --- Genetic Techniques --- Drug Discovery.
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Ribozyme Protocols brings together a broad spectrum of essential and easily reproducible techniques currently used by leading researchers to manipulate RNA molecules and to inactivate gene expression in vivo. The methods collected here range from theoretical design to clinical application and include the synthesis and assay of ribozymes, as well as their use both in vitro and in vivo. Each method is presented by an expert with substantial hands-on experience and in far greater detail than is generally to be found in the research literature. Ribozyme Protocols will help all investigators perform meaningful and successful experiments using ribozymes in both clinical and basic research, as well as launch fresh application initiatives. It is certain to be found indispensable not only by those workers new to the field, but also by experienced researchers who wish to expand their range of expertise.
Catalytic RNA -- Laboratory manuals. --- Electronic books. -- local. --- Nucleic acids -- Laboratory manuals. --- Catalytic RNA --- Genetic Techniques --- RNA, Catalytic --- RNA, Untranslated --- Investigative Techniques --- Enzymes --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Enzymes and Coenzymes --- RNA --- Chemicals and Drugs --- Nucleic Acids --- Nucleic Acids, Nucleotides, and Nucleosides --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Laboratory manuals --- RNA, Catalytic. --- Genetic Techniques. --- Laboratory manuals. --- Catalytic rna --- Genetic techniques. --- Rna, catalytic. --- Nucleic acids --- Polynucleotides --- Biomolecules --- Ribonucleic acid enzymes --- Ribozymes --- RNA enzymes --- Non-coding RNA --- Nucleases --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition
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DNA --- Pulsed-field gel electrophoresis --- Electrophoresis, Gel, Pulsed-Field --- Genetic Techniques. --- Analysis. --- Technique. --- methods. --- Pulsed-field gradient electrophoresis --- Gel electrophoresis --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition
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Molecular genetics --- Molecular cloning --- Nucleic acids --- Biotechnology. --- Cloning, Molecular --- Genetic Techniques. --- Nucleic Acids --- Molecular cloning. --- Molecular genetics. --- Analysis --- methods. --- analysis. --- Analysis. --- DNA cloning --- Gene cloning --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Biotechnologies --- Polynucleotides --- methods --- Molecular biology --- Cloning --- Genetic engineering --- Clone cells --- Genetic Counseling --- Genetic Phenomena --- Biomolecules --- Genetic Techniques --- Biotechnology --- analysis --- Molecular Cloning --- Cosmids --- Cloning, Organism --- Acids, Nucleic --- Nucleic Acid --- Acid, Nucleic
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