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Calpain Methods and Protocols
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ISBN: 0896036324 9786610820429 128082042X 1592590500 Year: 2000 Publisher: Totowa, NJ : Humana Press : Imprint: Humana,

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Abstract

In Calpain Methods and Protocols, John S. Elce and a seasoned team of principal investigators present a set of proven and easily followed protocols for studying calpain. The methods include in vitro techniques for the detection, expression, purification, and assay of µ- and m-calpain, supplemented with a wide range of system and tissue models for studying both the physiological functions and the effects of inhibitors on calpain. The systems used include neural tissue, kidney, liver, the eye, and membrane fusion in muscle and erythrocytes, each in connection with hypoxia or other injury. Among the analytical techniques employed are casein zymography, immunofluorescence, and calpain activity assays. The authors also examine specific substrates that have been proposed for the calpains. Highly practical and readily repeatable, Calpain Methods and Protocols offers investigators involved in basic and clinically oriented calpain research a gold-standard collection of powerful experimental tools for discovering the nature and function of calpains.

Methods in cell biology. 012. Volume 12
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ISBN: 0125641125 9786611514914 1281514918 0080859097 Year: 1975 Publisher: New York, NY ; San Francisco, CA ; London : Academic Press,

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The Local Cardiac Renin-Angiotensin Aldosterone System
Authors: ---
ISBN: 1280460458 9786610460458 0387278265 0387278257 Year: 2006 Volume: 20 Publisher: New York, NY : Springer US : Imprint: Springer,

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How exciting it is to see a field so well established as the ren- angiotensin system continue to grow and mature. Originally, following the original identification of renin by Tigerstedt and Bergman over 100 years ago, workers in this area spent years attempting to establish its role in experimental and renal hypertension. The early work by Goldblatt, in 1934, demonstrated that the placement of a clip around a renal artery was clearly related to the subsequent development of hypertension. However, it wasn't until the simultaneous finding by two different geographically separated teams, Page, et al, in the United States and Braun-Menendez, et al, in Argentina that the peptide angiotensin was identified. Thus, the rate-limiting enzyme renin was released from the kidney and catalyzed a biochemical cascade which was eventually shown to produce the elevated arterial pressure. Subsequently, many workers contributed to the elucidation of the concept and sequence of angiotensin I1 generation. Thus, the enzyme renin acted upon its protein substrate, produced in the liver, to liberate the decapeptide angiotensin I which, upon circulating through the pulmonary circulation, finally produced the potent octapeptide angiotensin. Several important subsequent findings demonstrated that angiotensin I1 promoted the release of the adrenal corticosteroid from that gland, thereby resulting in a larger system, the renin-angiotensin-aldosterone system. Further, this system demonstrated a classical biofeedback and the circulating octapeptide was shown to have additional biological activities in organs other than heart, vessels, kidney, adrenals, and even brain.

Thrombin : Physiology and Disease
Authors: --- --- --- ---
ISBN: 0387096361 1441918779 9786612824951 1282824953 038709637X 354009637X 3540347534 3540096361 9783540096368 9780387096360 9783540096375 Year: 2009 Volume: 19 Publisher: New York, NY : Springer New York : Imprint: Springer,

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Abstract

It has become increasingly evident in recent years that, apart from the key role that thrombin plays in the blood coagulation cascade, thrombin also elicits cellular actions via the activation of proteinase-activated receptors, which are present in many cell types. These effects of thrombin are seen in a variety of physiological as well as pathological phenomena, including vascular development and physiology, tumor progression and metastasis, neuronal functions, inflammation, angiogenesis. Thrombin: Physiology and Disease, edited by Michael E. Maragoudakis and Nikos E. Tsopanoglou, emphasizes the new developments in this important field of research and provides the basis for translating these findings into therapeutic targets.".

Keywords

Optical communications. --- Semiconductors. --- Thrombin --- Blood --- Thrombolytic therapy --- Receptors, G-Protein-Coupled --- Platelet Membrane Glycoproteins --- Receptors, Peptide --- Serine Endopeptidases --- Receptors, Proteinase-Activated --- Blood Coagulation Factors --- Biological Science Disciplines --- Receptors, Cell Surface --- Serine Proteases --- Membrane Glycoproteins --- Blood Proteins --- Endopeptidases --- Biological Factors --- Natural Science Disciplines --- Receptors, Thrombin --- Physiology --- Peptide Hydrolases --- Membrane Proteins --- Chemicals and Drugs --- Disciplines and Occupations --- Proteins --- Glycoproteins --- Hydrolases --- Amino Acids, Peptides, and Proteins --- Enzymes --- Enzymes and Coenzymes --- Human Anatomy & Physiology --- Electrical & Computer Engineering --- Medicine --- Health & Biological Sciences --- Engineering & Applied Sciences --- Oncology --- Telecommunications --- Physiological effect --- Coagulation --- Thrombin. --- Thrombolytic therapy. --- Physiological effect. --- Coagulation. --- Fibrinolytic therapy --- Thrombolysis --- Thrombosis --- Blood coagulation --- Clotting of blood --- Coagulation of blood --- Fibrinogenase --- Thrombase --- Thrombosin --- Chemotherapy --- Clotting --- Large scale systems --- Systèmes de grandes dimensions --- Systèmes de grandes dimensions --- Halvledarkomponenter --- Halvledarkomponenter. --- Medicine. --- Cancer research. --- Human physiology. --- Pharmacology. --- Epidemiology. --- Biochemistry. --- Biomedicine. --- Human Physiology. --- Cancer Research. --- Pharmacology/Toxicology. --- Biochemistry, general. --- Fibrinolysis --- Hemostasis --- Anticoagulants (Medicine) --- Coagulase --- Blood coagulation factors --- Hemostatics --- Serine proteinases --- Economics --- Computer engineering --- Mathematical models --- System theory --- Economie politique --- Congresses --- Modèles mathématiques --- Congrès --- Oncology. --- Toxicology. --- Diseases --- Public health --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Chemicals --- Pharmacology --- Poisoning --- Poisons --- Tumors --- Human biology --- Human body --- Composition --- Toxicology --- Drug effects --- Medical pharmacology --- Drugs --- Pharmacy --- Cancer research --- Economics - Mathematical models - Congresses --- Large scale systems - Congresses

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