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Focuses on research on all aspects of Saccharomyces and other yeast genera. The topics covered include: genetics; genomics; molecular biology; cell and developmental biology; biochemistry; physiology; pathobiology; biotechnology; ecology; taxonomy; and evolution. Abstracts and full-text (PDF only), 1997- ; abstracts only, 1996.

gisten --- Genetics --- yeast [fungi] --- Yeasts --- Yeast --- Levure --- genetics --- Periodicals. --- Périodiques --- Yeasts. --- 582.282.23 --- 579 --- 57 --- Fungal Proteins --- Saccharomycetineae. Yeasts. Brettanomyces --- Microbiology --- Biological sciences in general --- Periodicals --- YEASTS --- Agriculture Sciences --- Agricultural Biotechnology --- periodicals --- Soil Chemistry, Microbiology, Fertility & Fertilizers --- Agriculture Sciences. --- Agricultural Biotechnology. --- periodicals. --- 579 Microbiology --- 582.282.23 Saccharomycetineae. Yeasts. Brettanomyces --- Yeast. --- genetics. --- Nematospora --- Edible fungi --- Leavening agents --- Yeast-free diet --- Levures (Botanique)

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Microbiologie industrielle --- 579.66 --- Industrial and chemical microbiology --- 579.66 Industrial and chemical microbiology --- Fungi. --- Microorganisms. --- Bacteria --- Yeasts

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Pathogenic fungi --- Yeast fungi --- Yeast --- Congresses. --- Fungi, Pathogenic --- Yeasts --- Congresses --- Fungi --- Pathogenic microorganisms --- Medical mycology --- Basidiomycetes --- Blastomycetes --- Endomycetales

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In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.

Pichia --- Recombinant Proteins --- Recombinant proteins --- Saccharomyces cerevisiae --- Yeast fungi --- Yeasts --- biosynthesis --- Biotechnology --- Fungi --- Proteins --- Publication Formats --- Saccharomyces --- Saccharomycetales --- Publication Characteristics --- Ascomycota --- Eukaryota --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Organisms --- Laboratory Manuals --- Health & Biological Sciences --- Biomedical Engineering --- Baker Yeast --- S cerevisiae --- Baker's Yeast --- Brewer's Yeast --- Yeast, Baker's --- Yeast, Brewer's --- Baker Yeasts --- Baker's Yeasts --- Bakers Yeast --- Brewer Yeast --- Brewer's Yeasts --- Brewers Yeast --- Yeast, Baker --- Yeast, Bakers --- Yeast, Brewer --- Yeast, Brewers --- Yeasts, Baker --- Yeasts, Baker's --- Yeasts, Brewer's --- Proteins, Biosynthetic --- Proteins, Recombinant DNA --- Biosynthetic Proteins --- DNA Recombinant Proteins --- DNA Proteins, Recombinant --- Proteins, DNA Recombinant --- Proteins, Recombinant --- Recombinant DNA Proteins --- Recombinant Proteins, DNA --- Hansenula --- Hansenulas --- Pichias --- Yeast --- Genetically engineered proteins --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Ascomycetes --- Cochliobolus --- Sclerotinia --- Ascomycete --- Ascomycotas --- Sclerotinias --- Budding Yeast --- Endomycetales --- Endomycopsis --- Yeast, Budding --- Budding Yeasts --- Endomycetale --- Endomycopses --- Saccharomycetale --- Yeasts, Budding --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Fungi, Filamentous --- Molds --- Filamentous Fungi --- Filamentous Fungus --- Fungus --- Fungus, Filamentous --- Mold --- Genetic engineering --- Saccharomyce --- Medicine. --- Human genetics. --- Biomedicine. --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Clinical sciences --- Medical profession --- Life sciences --- Medical sciences --- Pathology --- Physicians

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Yeast Protocols intends to offer a selection of well-proven proto­ cols in cell and molecular biology, applicable to yeasts including, but certainly not exclusively aimed at, Saccharomyces cerevisiae. Saccharomyces cerevisiae and its very distant cousin, Schizosacc- romyces pombe, are of course now foremost model eukaryotic organisms, and the focus of wide-ranging experimental studies, especially those using molecular genetic techniques. Many of the lat­ ter, such as DNA sequencing and in vitro mutagenesis, are general DNA techniques, well covered in other volumes of Humana's Meth­ ods in Molecular Biology series, and elsewhere. The inclusion of a number of non-DNA techniques in this book is meant to reflect the resurgent interest in yeast cell biology sparked by the development of gene manipulation methods—for example, cellular localization of cloned gene products using microscopical techniques. The presentation of protocols follows the successful Methods in Molecular Biology series format, with a clear sequence of steps and extensive troubleshooting notes. It is our hope that these protocols will be useful not only to established members of the full-time research community, but also to the less experienced—first degree level and masters students undertaking project work, as well as PhD students starting their experimental programs; I am well aware that these young apprentice scientists are not always able to receive the supervision time that they, and indeed their supervisors, would like.

Yeasts --- Yeast fungi --- Levures (Botanique) --- physiology --- isolation & purification --- cytology --- Biotechnology --- Research --- Methodology --- Biotechnologie --- Yeast --- -Yeast fungi --- -Yeasts --- Fungi --- Basidiomycetes --- Blastomycetes --- Endomycetales --- Nematospora --- Edible fungi --- Leavening agents --- Yeast-free diet --- -Methodology --- Biotechnology. --- Methodology. --- physiology. --- cytology. --- isolation & purification. --- -Biotechnology --- Cytology. --- Isolation & purification. --- Physiology. --- Yeast biotechnology --- Microbial biotechnology --- Yeast industry --- Research&delete& --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Yeast fungi - Research - Methodology --- Yeast - Biotechnology