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Time-correlated single photon counting (TCSPC) is a remarkable technique for recording low-level light signals with extremely high precision and picosecond-time resolution. TCSPC has developed from an intrinsically time-consuming and one-dimensional technique into a fast, multi-dimensional technique to record light signals. So this reference and text describes how advanced TCSPC techniques work and demonstrates their application to time-resolved laser scanning microscopy, single molecule spectroscopy, photon correlation experiments, and diffuse optical tomography of biological tissue. It gives practical hints about constructing suitable optical systems, choosing and using detectors, detector safety, preamplifiers, and using the control features and optimising the operating conditions of TCSPC devices. Advanced TCSPC Techniques is an indispensable tool for everyone in research and development who is confronted with the task of recording low-intensity light signals in the picosecond and nanosecond range.
Photon correlation. --- Quantum optics. --- Optics --- Photons --- Quantum theory --- Correlation, Photon --- Light --- Quantum electrodynamics --- Quantum optics --- Spectrum analysis --- Scattering --- Chemistry, Physical organic. --- Optics, Lasers, Photonics, Optical Devices. --- Physical Chemistry. --- Solid State Physics. --- Spectroscopy and Microscopy. --- Chemistry, Physical organic --- Chemistry, Organic --- Chemistry, Physical and theoretical --- Lasers. --- Photonics. --- Physical chemistry. --- Solid state physics. --- Spectroscopy. --- Microscopy. --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Analysis, Spectrum --- Spectra --- Spectrochemical analysis --- Spectrochemistry --- Spectrometry --- Spectroscopy --- Chemistry, Analytic --- Interferometry --- Radiation --- Wave-motion, Theory of --- Absorption spectra --- Spectroscope --- Physics --- Solids --- Chemistry, Theoretical --- Physical chemistry --- Theoretical chemistry --- Chemistry --- New optics --- Light amplification by stimulated emission of radiation --- Masers, Optical --- Optical masers --- Light amplifiers --- Light sources --- Optoelectronic devices --- Nonlinear optics --- Optical parametric oscillators --- Qualitative --- Analytical chemistry
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This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research. The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.
Chemistry. --- Physical Chemistry. --- Optics and Electrodynamics. --- Atomic/Molecular Structure and Spectra. --- Measurement Science and Instrumentation. --- Signal, Image and Speech Processing. --- Chemistry, Physical organic. --- Chimie --- Chemistry --- Physical Sciences & Mathematics --- Physical & Theoretical Chemistry --- Photon correlation. --- Correlation, Photon --- Physical chemistry. --- Optics. --- Electrodynamics. --- Atomic structure. --- Molecular structure. --- Spectra. --- Physical measurements. --- Measurement. --- Light --- Quantum electrodynamics --- Quantum optics --- Spectrum analysis --- Scattering --- Classical Electrodynamics. --- Chemistry, Physical organic --- Chemistry, Organic --- Chemistry, Physical and theoretical --- Atomic structure . --- Molecular structure . --- Measurement . --- Signal processing. --- Image processing. --- Speech processing systems. --- Dynamics --- Physics --- Chemistry, Theoretical --- Physical chemistry --- Theoretical chemistry --- Computational linguistics --- Electronic systems --- Information theory --- Modulation theory --- Oral communication --- Speech --- Telecommunication --- Singing voice synthesizers --- Pictorial data processing --- Picture processing --- Processing, Image --- Imaging systems --- Optical data processing --- Processing, Signal --- Information measurement --- Signal theory (Telecommunication) --- Measuring --- Mensuration --- Mathematics --- Technology --- Metrology --- Physical measurements --- Measurements, Physical --- Mathematical physics --- Measurement --- Structure, Molecular --- Chemical structure --- Structural bioinformatics --- Structure, Atomic --- Atomic theory
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School management and organization --- Universities and colleges --- Education --- Administration. --- Finance. --- Children --- Education, Primitive --- Education of children --- Human resource development --- Instruction --- Pedagogy --- Schooling --- Students --- Youth --- Civilization --- Learning and scholarship --- Mental discipline --- Schools --- Teaching --- Training --- Colleges --- Degree-granting institutions --- Higher education institutions --- Higher education providers --- Institutions of higher education --- Postsecondary institutions --- Public institutions --- Education, Higher
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The sentinel lymph node concept is meeting with steadily growing interest and is being extended to the different sites of the primaries. In addition, the concept is being applied in an increasingly sophisticated manner. In this book the practical application of the sentinel node concept is evaluated from the points of view of pathology, radiodiagnosis and nuclear medicine diagnostics, surgical treatment and clinical oncology. The concept is analyzed for breast cancer, malignant melanoma, tumors of the face, oropharynx, lung, gastrointestinal and urogenital tract. The first part of the book describes the function and use of the nuclear medicine equipment, the tracers used, colloid solutions and modern developments in histological and immunohistochemical lymph node investigations, as well as possible pitfalls. The consequences of false-negative results are clearly delineated. In the second part, specific tumor-related problems are described.
Lymphatic metastasis --- Lymphatics --- Diagnosis. --- Cancer. --- Metastasis --- Cancer --- Hematology. --- Nuclear medicine. --- Pathology. --- Surgery. --- Gynecology. --- Nuclear Medicine. --- Gynaecology --- Medicine --- Generative organs, Female --- Surgery, Primitive --- Disease (Pathology) --- Medical sciences --- Diseases --- Medicine, Preventive --- Atomic medicine --- Radioisotopes in medicine --- Medical radiology --- Radioactive tracers --- Radioactivity --- Haematology --- Internal medicine --- Blood --- Physiological effect --- Gynecology .
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