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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact

enhancement --- genetics --- therapeutics --- CRISPR --- editorial

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RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in gene-editing research and technologies have enabled the CRISPR Cas9 system to become a popular tool for sequence-specific gene editing to correct and modify eukaryotic systems. In this book, we will focus on the mechanisms, applications, regulations (their pros and cons), and various ways in which RNAi-based methods and CRIPSR-Cas9 technology have stimulated the modulation of gene expression, thereby making them a promising therapeutic tool to treat and prevent complex diseases and disorders.

CRISPR-associated protein 9. --- Cas9 (CRISPR-associated protein 9) --- CRISPR/Cas9 --- Endonucleases --- Gene editing --- Life Sciences --- Molecular Genetics --- Genetics and Molecular Biology --- Genomics --- Biochemistry

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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact

CRISPR/Cas9 --- genome edited plants --- biosafety --- agriculture --- policy and legislation

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Genome Editing: A Practical Guide to Research and Clinical Applications is geared towards investigators interested in learning how to use CRISPR-Cas9-based technologies, with a focus on cardiovascular research and clinical applications. Covering a range of topics from the basics of genome editing to design considerations, to assessments and applications, this reference allows readers to get started and establish a full workflow from the beginning of the project to its full completion. With worked examples drawn from real-life experiments, as well as troubleshooting and pitfalls to avoid, the book serves as an essential reference for researchers and investigators in both cardiovascular and biomedical research.

Gene editing. --- CRISPR-associated protein 9. --- CRISPR (Genetics). --- Cardiovascular system --- Diseases --- Genetic aspects. --- Circulatory system --- Vascular system --- Blood --- Clustered Regularly Interspaced Short Palindromic Repeats (Genetics) --- Immunogenetics --- Nucleotide sequence --- Cas9 (CRISPR-associated protein 9) --- CRISPR/Cas9 --- Endonucleases --- Gene editing --- Editing, Gene --- Editing, Genome --- Genome editing --- Genetic engineering --- Circulation --- Methodology.

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Molecular genetics. --- Gene therapy. --- Genetic engineering. --- CRISPR-Cas Systems. --- Biotechnology. --- Genetic Therapy. --- Genetic Engineering.