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Requirements for a molecular biology laboratory. General methods for isolating and characterizing nucleic acids. Enzymatic techniques and recombinant DNA technology. Restriction enzymes. Growth and maintenance of bacteria. Genomic cloning. Preparation and characterization of RNA. Preparation of cDNA and the generation of cDNA libraries. Selection of clones from libraries. Identification and characterization of specific clones.

Biological techniques --- Enzymology --- Molecular biology --- Molecular cloning --- Clonage moléculaire --- Cloning, Molecular --- methods --- molecular cloning --- Expression des gènes --- gene expression --- Expérimentation en laboratoire --- Laboratory experimentation --- methods. --- Methods --- Methods. --- Clonage moléculaire --- Cloning, Molecular - methods --- NUCLEIC ACIDS --- DNA, RECOMBINANT --- MOLECULAR CLONING --- LABORATORY MANUALS --- TECHNIQUES --- Sequencing

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DNA --- Klonen --- 577.21 --- 575.08:577.21 --- 575.08:577.21 Genetic engineering, genetic manipulation, recombinant DNA --- Genetic engineering, genetic manipulation, recombinant DNA --- 577.21 Molecular mechanism of coding, storage and realization of inheritance information. Molecular genetics. Molecular biology of the gene --- Molecular mechanism of coding, storage and realization of inheritance information. Molecular genetics. Molecular biology of the gene --- Cloning, Molecular. --- DNA, Recombinant. --- Molecular cloning --- 57.086.8 --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Recombinant DNA Research --- Recombination Joint --- Genes, Spliced --- Recombinant DNA --- DNA Research, Recombinant --- Joint, Recombination --- Research, Recombinant DNA --- Spliced Genes --- DNA Transposable Elements --- Genetic Engineering --- Genetic Vectors --- Genomic Library --- Gene Library --- Artificial Gene Fusion --- Molecular Cloning --- Cosmids --- Cloning, Organism --- 57.086.8 Methods and equipment for studying living cells and tissues --- Methods and equipment for studying living cells and tissues --- Biological techniques --- Biochemical engineering --- Molecular biology --- Molecular cloning. --- Recombinant DNA. --- ADN --- cloning --- genetic engineering --- Clonage moléculaire --- ADN recombinant --- Erfelijke recombinatie. (Versch. onderwerpen) --- Clones moléculaires. (Mélanges) --- Recombinaison génétique. (Mélanges) --- Clonen (Moleculaire). (Versch. onderwerpen) --- DNA. --- DNA, Recombinant

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Eugenics --- Genetic engineering --- Human reproduction --- Eugénisme --- Génie génétique --- Reproduction humaine --- Moral and ethical aspects --- Aspect moral --- Ethics, Medical. --- Eugenics. --- Genetic Engineering. --- Reproduction. --- -Genetic engineering --- -Human reproduction --- -Human physiology --- Reproduction --- Reproductive health --- Reproductive rights --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Homiculture --- Race improvement --- Euthenics --- Heredity --- Involuntary sterilization --- Human Reproductive Index --- Human Reproductive Indexes --- Reproductive Period --- Human Reproductive Indices --- Index, Human Reproductive --- Indexes, Human Reproductive --- Indices, Human Reproductive --- Period, Reproductive --- Periods, Reproductive --- Reproductive Index, Human --- Reproductive Indices, Human --- Reproductive Periods --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- Cloning, Molecular --- DNA, Recombinant --- Industrial Microbiology --- Artificial Gene Fusion --- Organisms, Genetically Modified --- Animals, Genetically Modified --- Plants, Genetically Modified --- Negative Eugenics --- Positive Eugenics --- Eugenics, Negative --- Eugenics, Positive --- Selective Breeding --- Genetic Counseling --- Genetics, Medical --- Medical Ethics --- Medicine --- Professionalism --- Bioethics --- ethics --- Ethics --- Moral and ethical aspects. --- medical --- Genetic intervention. --- medical. --- -Moral and ethical aspects --- Medical. --- Eugénisme --- Génie génétique