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The ability to introduce macromolecules into animal cells, includ ing DNA, RNA, proteins, and other bioactive compounds has facili tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene structure and function, and it has impor tant practical applications in both biotechnology and biomedicine, as evidenced by the current intense interest in gene therapy. Although DNA and other macromolecules may be introduced into cells by a variety of methods, including chemical treatments and microinjection, el- troporation has proven to be simpler to perform, more efficient, and effective with a wider variety of cell types than other techniques. The early and broad success of electric field-mediated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, including RNA, enzymes, antibodies, and analytic dyes. Animal Cell Electroporation and Electrofusion Protocols begins with three chapters that describe the theoretical and practical aspects of electroporation, including a review of the commercially available instrumentation. These introductory chapters will be of particular inter est to those new to electric field technologies and to those developing protocols for as yet untested species or cell types. Nineteen chapters follow that present well-tested protocols for electroporation of proteins and DNA into insect, fish, and mammalian cells.
Animal cell biotechnology. --- Electrofusion. --- Electronic books. -- local. --- Electroporation. --- Electroporation --- Electrofusion --- Animal cell biotechnology --- Electrochemical Techniques --- Clinical Laboratory Techniques --- Cell Physiological Processes --- Investigative Techniques --- Cytological Techniques --- Cell Fusion --- Genetic Techniques --- Cell Physiological Phenomena --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Phenomena and Processes --- Biology --- Cytology --- Health & Biological Sciences --- Animal cells in biotechnology --- Animal biotechnology --- Bioelectrochemistry --- Membrane fusion --- Technique --- Cytology. --- Animal genetics. --- Cell Biology. --- Animal Genetics and Genomics. --- Genetics --- Cell biology --- Cellular biology --- Cells --- Cytologists
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Vision --- Eye --- Oeil --- Genetic aspects --- Periodicals --- Cytology --- Molecular aspects --- Aspect génétique --- Périodiques --- Cytologie --- Aspect moléculaire --- Cells. --- Molecular Biology. --- Vision. --- Œil --- genetics. --- Eyesight --- Seeing --- Sight --- Light Signal Transduction, Visual --- Ocular Vision --- Visual Light Signal Transduction --- Visual Phototransduction --- Visual Transduction --- Phototransduction, Visual --- Transduction, Visual --- Biochemical Genetics --- Biology, Molecular --- Genetics, Biochemical --- Genetics, Molecular --- Molecular Genetics --- Biochemical Genetic --- Genetic, Biochemical --- Genetic, Molecular --- Molecular Genetic --- Cell --- Eyeball --- Eyes --- Visual system --- Senses and sensation --- Blindfolds --- Physiological optics --- Ophthalmology --- Visual Perception --- Genetic Phenomena --- Cell Biology --- Face --- Photoreceptors --- Vision, Ocular. --- Vision, Ocular --- Aspect moléculaire --- Aspect génétique --- Cytology. --- molecular biology --- ophthalmology --- genetics --- biochemistry --- cell biology --- Cells --- Molecular Biology --- cells. --- molecular biology. --- vision. --- genetics --- Biological Sciences --- animal genetics --- cytogenetics --- gender --- genetic equilibrium --- genetic factors --- genetic relationships --- genetic selection --- genetic stability --- genetic structure --- genetic traits --- genetic vectors --- genomics --- human genetics --- immunogenetics --- inheritance (genetics) --- landscape genetics --- maternal effect --- metabolomics --- microbial genetics --- molecular genetics --- nutrigenomics --- paternal effect --- phenomics --- phenotype --- plant genetics --- population genetics --- proteomics --- quantitative genetics --- reproductive fitness --- sex determination --- transcriptomics --- biotechnology --- breeding --- genetic models --- genetic resources --- geneticists --- eyesight --- visual phototransduction --- visual transduction --- ocular physiology --- sensation --- color vision --- night vision --- eye diseases --- ophthalmology --- photoreceptors --- sensory properties --- vision disorders --- visual cortex --- visual perception --- bioinformatics --- molecular dynamics --- molecular epidemiology --- molecular models --- molecular systematics --- cell biology --- acinar cells --- adipocytes --- aleurone cells --- antigen-presenting cells --- bacteriocytes --- blood cells --- bone marrow cells --- bundle sheath cells --- chondrocytes --- chromaffin cells --- chromatophores --- coelomocytes --- cultured cells --- endothelial cells --- epithelial cells --- eukaryotic cells --- fibroblasts --- germ cells --- giant cells --- gonadotropic cells --- granulosa cells --- guard cells --- hemocytes --- hepatocytes --- islets of Langerhans --- keratinocytes --- kidney cells --- Leydig cells --- luteal cells --- mast cells --- myocytes --- neoplasm cells --- neurosecretory cells --- nurse cells --- osteoblasts --- osteoclasts --- phagocytes --- plant root cells --- prokaryotic cells --- Schwann cells --- Sertoli cells --- somatic cells --- splenocytes --- spores --- stem cells --- stromal cells --- tenocytes --- teratocytes --- thecal cells --- vegetative cells --- artificial cells --- cell transplantation --- cell viability --- cellular microenvironment --- Cellules. --- sight (sense) --- Organisms
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