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The design and production of novel peptides and proteins occupy pivotal positions in science and technology and will continue to do so in the 21st century. Protein Engineering and Design outlines the rapid advances in computer-based modeling, protein engineering, and methods needed for protein and peptide preparation and characterization. This indispensable reference lays the groundwork for understanding this multidisciplinary activity while providing an introduction for researchers and students to the field of protein design.Key Features* Introduces and defines the techniqu

Protein engineering. --- Proteins. --- Protein engineering --- Genetic Engineering --- Models, Theoretical --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Genetic Techniques --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Protein Engineering --- Proteins --- Models, Molecular --- Health & Biological Sciences --- Biomedical Engineering --- Biochemical engineering. --- Bio-process engineering --- Bioprocess engineering --- Engineering, Protein --- Protein design --- Design --- Biochemistry --- Biotechnology --- Chemical engineering --- Biochemical engineering --- Genetic engineering

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Pharmacognosy --- Pharmacopeias. Codexes. Formularia. Recepy --- Toxicology --- Descriptive organic chemistry --- Chemicals --- Drugs --- Biomolecules --- Pharmaceutical Preparations --- Dictionaries, Pharmaceutic --- Chemistry --- Chemicals and Drugs Category --- Dictionaries. --- Dictionaries --- Médicaments --- Produits chimiques --- Biomolécules --- Élément chimique --- Elements --- Produit chimique --- chemi cals --- Structure chimique --- chemical structure --- Synthèse chimique --- Chemical synthesis --- Réaction chimique --- chemical reactions --- Pharmaceutical Preparations. --- -Chemicals --- -Drugs --- -drugs --- chemicaliën --- Medicaments --- Medications --- Medicine (Drugs) --- Medicines (Drugs) --- Pharmaceuticals --- Prescription drugs --- Bioactive compounds --- Medical supplies --- Pharmacopoeias --- Chemotherapy --- Materia medica --- Pharmacy --- Chemical compounds --- Compounds, Chemical --- Biological molecules --- Molecules --- Pharmaceutic Preparations --- Pharmaceutical Products --- Preparations, Pharmaceutical --- Preparations, Pharmaceutic --- Products, Pharmaceutical --- Drug Dosage Calculations --- Médicaments --- Biomolécules --- drugs --- Pharmacology --- Molecular biology --- Dictionnaires --- Dictionnaires anglais --- Monograph --- Drug --- Pharmaceutical --- Pharmaceutical Preparation --- Pharmaceutical Product --- Preparation, Pharmaceutical --- Product, Pharmaceutical --- Chemicals - Encyclopedias --- Drugs - Encyclopedias --- Biomolecules - Dictionaries --- Pharmaceutical Preparations - dictionary --- Chemistry - dictionary --- Drugs - Dictionaries --- Chemicals - Dictionaries --- Biomolecules - Dictionaries. --- Chemicals and Drugs Category - encyclopedias --- PHARMACEUTICAL PREPARATIONS --- INDUSTRIAL CHEMICALS --- DRUGS --- CLASSIFICATION

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Interest in epitope mapping, or finding out where antibodies bind to their antigens, is by no means restricted to immunologists, but is shared by biolo­ gists from a wide range of disciplines in which antibodies are used as molecu­ lar reagents. The epitope mapper may be interested in studying protein-protein interactions, in developing an immunoassay, in producing protective peptide vaccines, in investigating the pathogenesis of autoimmune diseases, or in defining protein topology in intact cells or organelles, to mention only a few of the possibilities. The aim of Epitope Mapping Protocols is to provide both a useful range of alternative practical methods for the experienced mapper and a fairly com­ prehensive introduction for someone embarking on antibody production and mapping for the first time. Contributors were encouraged to illustrate their protocols with results from their own research and most of them elected to do so. After an introductory chapter, the protocols are arranged in three groups: The first group of twelve methods uses (or can use) whole, native antigens and may therefore be suitable for conformational epitopes; the second group of five uses peptides or peptide libraries; and the third group of eleven requires antigen expressed from recombinant DNA. Within this last group, methods more likely applicable to conformational epitopes are placed first.

Antigenic determinants --- Antigen-antibody reactions --- Antigen-Antibody Reactions --- Epitope Mapping --- Epitopes --- Antigens --- Immunologic Tests --- Protein Interaction Mapping --- Immune System Processes --- Immune System Phenomena --- Immunologic Techniques --- Molecular Probe Techniques --- Biological Factors --- Laboratory Techniques and Procedures --- Investigative Techniques --- Phenomena and Processes --- Diagnosis --- Chemicals and Drugs --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Microbiology & Immunology --- Biology --- Health & Biological Sciences --- Research --- Methodology --- Methodology. --- Life sciences. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition

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Environmental protection. Environmental technology --- Toxicology --- Toxicologie --- Écologie --- ecology --- Substance toxique --- toxic substances --- Impact sur l'environnement --- Environmental impact --- Santé publique --- public health --- Toxicité --- toxicity --- Mesure --- Measurement --- Polluant --- pollutants --- Pesticide --- pesticides --- Biodégradation --- Biodegradation --- Bioaccumulation --- glossaire --- glossary --- Environmental toxicology. --- Environmental toxicology --- Environmental Pollutants --- Environmental Pollution --- Public Health --- Toxic Actions --- Chemical Actions and Uses --- Environment and Public Health --- Health Care --- Chemicals and Drugs --- Environmental Exposure --- Hazardous Substances --- Health & Biological Sciences --- Toxicology & Public Health --- 615.9 --- Ecotoxicology --- Pollutants --- Pollution --- Environmental health --- General toxicology. General studies of poisons and poisoning (intoxication) --- 615.9 General toxicology. General studies of poisons and poisoning (intoxication) --- Toxicology. --- Biodegradation.

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Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this i

Biochemistry. --- Bioconjugates. --- Bioconjugates--Synthesis. Biochemistry--methods. Proteins--chemistry. Peptides--chemistry. Nucleic Acids--chemistry. --- chemistry. --- methods. --- Nucleic acids. --- Peptides. --- Proteins. --- Synthesis. --- Bioconjugates --- Natural Science Disciplines --- Investigative Techniques --- Biological Science Disciplines --- Nucleic Acids, Nucleotides, and Nucleosides --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Disciplines and Occupations --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Biochemistry --- Chemistry --- Methods --- Peptides --- Nucleic Acids --- Proteins --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Synthesis --- Bioconjugate chemistry --- Conjugated biomolecules --- Conjugation biochemistry --- Conjugation chemistry --- Biomolecules --- Metabolic conjugation --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Medical sciences --- Composition --- ELSEVIER-B EPUB-LIV-FT