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Mice --- Mice --- Mice as laboratory animals --- Souris --- Souris (Animaux de laboratoire) --- Genetics --- Variation. --- Génétique

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Nucleic acid hybridization is central to a range of important techniques in widespread use in molecular biology, but requires careful optimization in order to generate reliable experimental data. This book provides essential information on nucleic acid hybridization for the active researcher. It includes invaluable guidance and key data on for example labeling probes, blotting procedures, hybridization conditions and detection systems. The Essential Data series provides rapid access to the core data required by researchers on a daily basis, in convenient pocket-sized volumes.

Acide nucléique --- Nucleic acids --- Hybridation --- Hybridization --- Matériel de laboratoire --- Laboratory equipment --- methods --- Échantillonnage --- Sampling --- Électrophorèse --- Electrophoresis --- Nucleic acid hybridization. --- Nucleic acids. --- Hybridization. --- Laboratory equipment. --- methods. --- Sampling. --- Electrophoresis.

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Mars 1938. Comme d'autres ouvrages du même type, le pont métallique de Hasselt s'effondre dans les eaux du canal Albert. Immédiatement, les Ponts et Chaussées créent la Section spéciale d'étude des matérieux métalliques chargée de cerner les causes de ces accidents. A partir de 1940, cette section spéciale est intégrée au bureau des Ponts. Jusqu'en 1980, elle est implantée au Val-Benoît, à l'Institut de génie civilde Liège où elle produit ses activités de contrôle et de recherches. Mai 1996. La division du Contrôle technique (I.G.42) - qui fait partie du Ministère Wallon de l'Equipement et des Transports - a succédé à la section liégeoise du bureau des Ponts. Elle a également intégré le personnel de l'ancienne section francophone de l'Institut géotechnique de l'Etat. Ensemble ces ingénieurs continuent de veiller à la qualité des ouvrages d'artexécutés pour le MET. Donc de jouer un rôle en vue dans le processus de développement technique. Ce livre retrace l'histoire de la division du Contrôle technique et, surtout, de ses devancières. Une histoire qui est celle - aussi - des techniques de construction des ouvrages d'art. Dans une seconde partie, cette publication détaille l'érection du nouveau bâtiment de l'I.G.42, connecté au premier sur le site de la Côte d'Or, à Liège-Sclessin. Un bâtiment qui allie esthétisme et fonctionnalité. Une infrastructure qui offre un cadre professionnel de qualité et qui constitue un gage indéniable de la performance des agents qui y travaillent. De leur épanouissement aussi.

Architecture --- Bouwkunde --- Bouwkunst --- Bouwnijverheid --- Construction --- Génie civil --- Liège (province) --- Luik (provincie) --- Construction de bâtiments --- Building construction --- Laboratoire --- laboratories --- Matériel de laboratoire --- Laboratory equipment --- Sécurité du travail --- Safety at work --- Pont --- Construction métallique --- Résistance des matériaux --- Corrosion --- Vieillissement des matériaux --- Ouvrage d'art --- Norme --- Construction en béton --- Bâtiment public --- Liège --- Hasselt --- Liège (Belgique) --- Laboratoires --- Ouvrages d'art --- Belgique

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Nuclear Magnetic Resonance --- Proteins --- Nuclear magnetic resonance spectroscopy --- Protéines --- Spectroscopie de la résonance magnétique nucléaire --- methods. --- analysis. --- Analysis --- Laboratory manuals. --- Laboratory manuals --- Analyse --- Manuels de laboratoire --- methods --- laboratory manuals. --- analysis --- Magnetic Resonance Spectroscopy --- Protéines --- Spectroscopie de la résonance magnétique nucléaire --- Methods

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Advances in bioscience research usually arise as a result of the continu­ ing refinement of existing technologies. However, there are a number of occa­ sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu­ tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech­ nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac­ tion. This technique, first reported in 1985 by MuUis and his colleagues, pro­ vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.

Molecular biology --- Analytical biochemistry --- Polymerase Chain Reaction --- Sequence Analysis, DNA --- Polymerase chain reaction --- Réaction en chaîne de la polymérase --- methods --- laboratory manuals --- Laboratory manuals --- Manuels de laboratoire --- Nucleic Acid Amplification Techniques --- Genetic Techniques --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Laboratory manuals. --- Réaction en chaîne de la polymérase --- Polymerization --- Polymerisation --- Polymers --- Polymers and polymerization --- Synthesis of polymers --- Chemical reactions --- Synthesis --- laboratory manuals. --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Cell biology.