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In Cytochrome P450 Protocols, Ian Phillips and Elizabeth Shephard assemble a comprehensive collection of cutting-edge techniques for the investigation of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification and enzymatic assays, expression in heterologous systems, and the production and use of antibodies, as well as methods for quantification of gene expression, transfection of hepatocytes, and for the investigation of DNA-protein interactions and genetic polymorphisms. In addition, because of the growing importance of in vitro systems in pharmacological toxicology, the book contains techniques for the culture of rodent and human hepatocytes and human epidermis. Cytochrome P450 induction as a biomarker for environmental pollution and the generation of mice with targeted gene disruptions complete this exhaustive collection of core techniques. Cytochrome P450 Protocols includes in one volume both state-of-the-art and classic methods that have not been superseded but remain extremely useful. The collection provides both novice and experienced researchers across many fields-toxicology, pharmacology, environmental biology, biochemistry, and molecular biology-all the tools needed to elucidate the crucial biological role played by cytochromes P450 in the metabolism of therapeutic drugs, chemical carcinogens, and environmental pollutants.

Cytochrome P-450 -- Laboratory manuals. --- Cytochrome P-450 --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Laboratory manuals --- Laboratory manuals. --- Cytochromes --- Metalloenzymes --- Monooxygenases --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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RNA Isolation and Characterization Protocols offers biological investigators a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The authoritative researchers writing here also include detailed protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility. RNA Isolation and Characterization Protocols gives today's bench scientists the single best collection of easily reproducible, step-by-step RNA techniques now available. This book will quickly become indispensable for everyone seeking to manipulate RNA and help set the stage for the coming revolution in RNA-based diagnostic techniques.

RNA --- Purification --- Laboratory manuals --- Analysis --- Nucleic Acids --- Nucleic Acids, Nucleotides, and Nucleosides --- Chemicals and Drugs --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- -RNA --- -Ribonucleic acid --- Ribose nucleic acid --- Nucleic acids --- Ribose --- -Laboratory manuals --- Laboratory manuals. --- -Analysis --- Ribonucleic acid --- Analysis&delete& --- Purification&delete& --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- RNA - Purification - Laboratory manuals --- RNA - Analysis - Laboratory manuals

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Proteins: Analysis and Design focuses solely on individual experimental approaches, rather than on specific classes of proteins. The book provides insight into the important issues in protein science and how one can cope with them. These include all issues which explore the detailed relationship of protein structure to function. Key Features:* Provides problems and technical solutions* Includes posttranslational modifications* Uses synthetic peptides as biological models* Details mutagenesis and protein engineering* Covers design of protein structure and func

Protein engineering. --- Proteins. --- Proteins - Analysis. --- Proteins. Proteins--Analysis. Protein engineering. --- Proteins --- Protein engineering --- Amino Acids, Peptides, and Proteins --- Genetic Engineering --- Molecular Conformation --- Molecular Structure --- Genetic Techniques --- Chemicals and Drugs --- Biochemical Phenomena --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemical Phenomena --- Phenomena and Processes --- Protein Engineering --- Protein Conformation --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Analysis --- Analysis. --- Engineering, Protein --- Protein design --- Proteids --- Design --- Biochemical engineering --- Genetic engineering --- Biomolecules --- Polypeptides --- Proteomics

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Comprehensive and state-of-the-art, Bioluminescence Methods and Protocols brings together a collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors-all hands-on researchers who have perfected their protcols-illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wide range of pollutants, to microbiological detection and analysis, and the molecular biological manipulation of lux, luc, and gfp structural genes. Step-by-step methods for the construction of other recombinant organisms are also included. Bioluminescence Methods and Protocols represents the state-of-the-art in luminescent assays. Its cutting-edge techniques, diversely illustrated applications, and detailed instructions will make it the standard reference for today's investigators who want to adapt established techniques to their purposes or apply them to a wide range of clinical, microbiological, environmental, toxicological, and analytical problems.

Molecular biology --- Enzymes --- Micro-organisme --- microorganisms --- Propriété optique --- Optical properties --- Biochimie --- biochemistry --- Génie biochimique --- biochemical engineering --- Variation génétique --- genetic variation --- Oxydation --- Oxidation --- Synthèse protéique --- protein synthesis --- Contamination biologique --- Biological contamination --- Contamination chimique --- Chemical contamination --- Surveillance de l'environnement --- Biochemistry. --- Bioluminescence assay. --- Electronic books. -- local. --- Bioluminescence assay --- Photometry --- Molecular Probe Techniques --- Luminescent Measurements --- Biosensing Techniques --- Investigative Techniques --- Chemistry Techniques, Analytical --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Human Anatomy & Physiology --- Animal Biochemistry --- Health & Biological Sciences --- Life sciences. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition --- ATP luminescence assay --- Bioluminescent assay --- Biological assay --- Application --- Luciferase