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RNA Isolation and Characterization Protocols offers biological investigators a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The authoritative researchers writing here also include detailed protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility. RNA Isolation and Characterization Protocols gives today's bench scientists the single best collection of easily reproducible, step-by-step RNA techniques now available. This book will quickly become indispensable for everyone seeking to manipulate RNA and help set the stage for the coming revolution in RNA-based diagnostic techniques.

RNA --- Purification --- Laboratory manuals --- Analysis --- Nucleic Acids --- Nucleic Acids, Nucleotides, and Nucleosides --- Chemicals and Drugs --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- -RNA --- -Ribonucleic acid --- Ribose nucleic acid --- Nucleic acids --- Ribose --- -Laboratory manuals --- Laboratory manuals. --- -Analysis --- Ribonucleic acid --- Analysis&delete& --- Purification&delete& --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- RNA - Purification - Laboratory manuals --- RNA - Analysis - Laboratory manuals

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This much-needed collection of Tumor Marker Protocols presents cutting-edge methods for the detection and discovery of new cancer markers, particularly molecular tumor markers. Each method is fully described by a hands-on expert who uses it regularly in the laboratory and has perfected it to a high degree of ready reproducibility. The book also places special emphasis on statistical considerations in the analysis of tumor markers, recent discoveries in the field of serum and tissue marker proteins, cytogenetic markers, molecular markers, and markers for the early detection of cancer. Tumor Marker Protocols offers many promising opportunities for transferring new technologies from the realm of basic science to that of clinical practice. The well-honed procedures detailed here will enable cancer researchers and clinical oncologists alike to realize the promise of these powerful new diagnostic strategies for the detection, therapy, and successful prevention of cancer.

Tumor markers --- Tumor Markers, Biological --- Neoplasms --- Diagnosis --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Biological Markers --- Diseases --- Biological Factors --- Chemicals and Drugs --- Oncology --- Pathology --- Medicine --- Health & Biological Sciences --- Tumor markers. --- Tumors --- Diagnosis. --- Biological markers (Oncology) --- Cancer markers --- Markers, Tumor --- Tumor associated markers --- Biochemical markers --- Pathology. --- Disease (Pathology) --- Medical sciences --- Medicine, Preventive --- TUMOR MARKERS, BIOLOGICAL --- NEOPLASMS --- DIAGNOSIS

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This much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins, and their various conjugates. The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities.

Electronic books. -- local. --- Immunochemistry -- Methodology. --- Microbiology. --- Immunochemistry --- Antibodies --- Immunoglobulins --- Biochemistry --- Biological Science Disciplines --- Serum Globulins --- Chemistry --- Immunoproteins --- Blood Proteins --- Globulins --- Natural Science Disciplines --- Proteins --- Disciplines and Occupations --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Microbiology & Immunology --- Biology --- Health & Biological Sciences --- Methodology --- Methodology. --- Microbial biology --- Microorganisms --- Immunity --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Cells --- Cytologists

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Proteins: Analysis and Design focuses solely on individual experimental approaches, rather than on specific classes of proteins. The book provides insight into the important issues in protein science and how one can cope with them. These include all issues which explore the detailed relationship of protein structure to function. Key Features:* Provides problems and technical solutions* Includes posttranslational modifications* Uses synthetic peptides as biological models* Details mutagenesis and protein engineering* Covers design of protein structure and func

Protein engineering. --- Proteins. --- Proteins - Analysis. --- Proteins. Proteins--Analysis. Protein engineering. --- Proteins --- Protein engineering --- Amino Acids, Peptides, and Proteins --- Genetic Engineering --- Molecular Conformation --- Molecular Structure --- Genetic Techniques --- Chemicals and Drugs --- Biochemical Phenomena --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemical Phenomena --- Phenomena and Processes --- Protein Engineering --- Protein Conformation --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Analysis --- Analysis. --- Engineering, Protein --- Protein design --- Proteids --- Design --- Biochemical engineering --- Genetic engineering --- Biomolecules --- Polypeptides --- Proteomics

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Christopher Redfern brings together in Retinoid Protocols a comprehensive collection of key biochemical and molecular methods for retinoid research. These easily reproducible techniques range from methods of handling and analyzing retinoids to advanced protocols for gene targeting, from binding protein function in transgenic animals to the study of nuclear retinoid receptors. In addition, the book makes accessible to all retinoid researchers such advanced techniques as cloning via RT-PCR, fluorimetry methods, recombinant protein purification and characterization, the use of antisense oligonucleotides to study the role of CRABPs, immunological and in situ hybridization methods, photoaffinity labeling, gel-shift analysis, and differential display. Many of the chapters in Retinoid Protocols represent landmarks in retinoid methodology because they give step-by-step instructions for techniques central to understanding cellular control by retinoids. These indispensable techniques will be fundamental for all those working with retinoids, and so will play a key role in the development of new drugs and therapies for treating human disease.

Retinoids --- Laboratory manuals --- Analysis --- Genetic Techniques --- Receptors, Retinoic Acid --- Investigative Techniques --- Carotenoids --- Receptors, Cytoplasmic and Nuclear --- Transcription Factors --- Proteins --- Polyenes --- Pigments, Biological --- Terpenes --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- DNA-Binding Proteins --- Cyclohexenes --- Biological Factors --- Amino Acids, Peptides, and Proteins --- Alkenes --- Cyclohexanes --- Hydrocarbons --- Organic Chemicals --- Hydrocarbons, Acyclic --- Cycloparaffins --- Chemicals and Drugs --- Hydrocarbons, Alicyclic --- Hydrocarbons, Cyclic --- Laboratory manuals. --- Vitamin A analogs --- Vitamin A congeners --- Vitamin A derivatives --- Diterpenes --- Interphotoreceptor retinoid-binding protein --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Retinoids - Laboratory manuals --- Retinoids - Analysis - Laboratory manuals