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From Reviews of the First Edition "...a must for researchers in protein chemistry and especially those with less experience..." -Doody's Health Sciences Book Review Journal "...definitely an indispensable benchtop handbook...highly recommended for all levels of research within the field."-Bioseparation "...a very useful tool for daily work." -Nahrung-Food Since publication of the bestselling first edition of John Walker's widely acclaimed Protein Protocols Handbook in 1996, there have been many highly significant developments in protein biochemistry. This greatly enhanced second edition introduces 60 critically important new chapters dealing with topics and techniques that have emerged since the first edition, as well as significantly updating the remaining articles. The new chapters cover many rapidly developing areas, particularly the application of mass spectrometry in protein characterization, as well as the now well-established 2-D PAGE technique in proteomics. The section on glycoprotein analysis has also been significantly expanded, and methods for the production of single-chain and phage-displayed antibodies have been added to the section on antibodies. Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The authors are commonly the techniques' originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity. Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Second Edition is today's indispensable benchtop manual and guide-not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armametarium of techniques in the urgent search for rapid and still more robust results.

Analytical biochemistry --- Proteins --- Peptides --- Protéines --- analysis --- Analysis --- Laboratory manuals --- Laboratory Manuals --- Analyse --- Manuels de laboratoire --- Biochemistry. --- Life sciences. --- Proteins -- Analysis -- Laboratory manuals. --- Proteomics. --- Science. --- Amino Acids, Peptides, and Proteins --- Molecular Conformation --- Investigative Techniques --- Molecular Structure --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemicals and Drugs --- Biochemical Phenomena --- Chemical Phenomena --- Phenomena and Processes --- Protein Conformation --- Chemistry Techniques, Analytical --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Protéines --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition --- Immunology. --- Immunobiology --- Life sciences --- Serology --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- PROTEINS --- ANALYSIS --- LABORATORY MANUALS

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The ways in which humans interact with computers will change dramatically in the coming years. In this book, the field's leading experts preview that future, focusing on critical technical challenges and opportunities that will define Human-Computer Interaction research for years and decades to come. Editor John M. Carroll, a leader of the HCI community, has assembled essays that anticipate tomorrow's state-of-the-art -- and its implications for users, professionals, and society. These essays cover every area of research, including models, theories, and frameworks; usability engineering; user interface software and tools; HCI for collaborative applications; HCI for multimedia and hypermedia; integrating real and virtual worlds; and HCI's impact on society. Discover advanced cognitive models for evaluating user interfaces; preview the future of user interface software tools; and learn how user interfaces can support innovation. Preview tomorrow's intelligent interfaces, recommender systems, and tangible user interfaces; as well as interface solutions for digital libraries and ubiquitous computing systems. Carroll provides cogent introductions to each essay, as well as a detailed preface offering an overview of the entire field.

Human-computer interaction --- User-centered system design --- Human-computer interaction. --- #SBIB:309H1720 --- #SBIB:309H03 --- Attitude to Computers --- Attitude to Computer --- Computer, Attitude to --- Computers, Attitude to --- to Computer, Attitude --- to Computers, Attitude --- Computers --- Computer-human interaction --- Human factors in computing systems --- Interaction, Human-computer --- Human engineering --- User interfaces (Computer systems) --- Informatiekunde, informatie management --- Systeemtheorie: cybernetica systeemleer, mathematische informatietheorie

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This book is a provocative contribution to contemporary ethical theory challenging foundational conceptions of character that date back to Aristotle. John Doris draws on behavioral science, especially social psychology, to argue that we misattribute the causes of behavior to personality traits and other fixed aspects of character rather than to the situational context. More often than not it is the situation not the nature of the personality that really counts. The author elaborates the philosophical consequences of this research for a whole array of ethical theories and shows that, once rid of the misleading conception of motivation, moral psychology can support more robust ethical theories and more humane ethical practices.

Character. --- Personality. --- Character --- Ethics --- Personality --- Personal identity --- Personality psychology --- Personality theory --- Personality traits --- Personology --- Traits, Personality --- Psychology --- Individuality --- Persons --- Self --- Temperament --- Deontology --- Ethics, Primitive --- Ethology --- Moral philosophy --- Morality --- Morals --- Philosophy, Moral --- Science, Moral --- Philosophy --- Values --- Ethics.

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John Rist surveys the history of ethics from Plato to the present and offers a vigorous defence of an ethical theory based on a revised version of Platonic realism. In a wide-ranging discussion he examines well-known alternatives to Platonism, in particular Epicurus, Hobbes, Hume and Kant as well as contemporary 'practical reasoners', and argues that most post-Enlightenment theories of morality (as well as Nietzschean subversions of such theories) depend on an abandoned Christian metaphysic and are unintelligible without such grounding. He also argues that contemporary choice-based theories, whether they take a strictly ethical or more obviously political form, are ultimately arbitrary in nature. His lively and accessible 2001 study is informed by a powerful sense of philosophical history, and will be of interest to both students and scholars of ethics.

Ethics. --- Realism. --- Ethics --- Empiricism --- Philosophy --- Universals (Philosophy) --- Conceptualism --- Dualism --- Idealism --- Materialism --- Nominalism --- Positivism --- Rationalism --- Deontology --- Ethics, Primitive --- Ethology --- Moral philosophy --- Morality --- Morals --- Philosophy, Moral --- Science, Moral --- Values --- History. --- Moral realism. --- Realism --- Arts and Humanities

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