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Genetically very similar to the human species, mice play an important role in biomedical research and have served as experimental models for a wide variety of pathologies, including cancer, cardiovascular diseases, and behavioral disorders. In Transgenic Mouse Methods and Protocols, Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularly for generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented. Care is taken to make clear the details of the available approaches, as well as their limitations. Up-to-date and highly practical, Transgenic Mouse Methods and Protocols demonstrates clearly for both novice and expert investigators how to make novel transgenic mouse models, and how to use them effectively to understand the role of gene function in human health and disease.
Molecular biology --- Transgenic mice --- Biology --- Mice --- Transgenic animals --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Cells --- Cytologists
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DescriptionA transgenic animal is one that is genetically modified to carry genes from another species. Transgenic species can be raised to carry potentially useful genes from a variety of species. While the topics of genetic engineering and cloning are controversial, the reality is that these technologies offer tremendous benefits to society - from offering a framework for developing and screening medical therapies, to enhancing the safety and nutrition of the food we eat. Importance of TopicOne potential application of research into transgenic animal technology is the creatio
Animal embryology and growth --- Histology. Cytology --- Animal genetic engineering --- Transgenic animals --- Animal biotechnology --- Génie génétique animal --- Animaux transgéniques --- Biotechnologie animale --- Animals --- Genetic engineering
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The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms. Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes.
Animal genetic engineering --- Transgenic animals --- Génie génétique animal --- Animaux transgéniques --- Laboratory manuals --- Manuels de laboratoire --- Animal genetic engineering -- Laboratory manuals. --- Electronic books. -- local. --- Transgenic animals -- Laboratory manuals. --- Genetic Engineering --- Animals, Genetically Modified --- Transformation, Genetic --- Organisms, Genetically Modified --- Recombination, Genetic --- Animal Population Groups --- Genetic Techniques --- Investigative Techniques --- Animals --- Genetic Processes --- Organism Forms --- Organisms --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Genetic Phenomena --- Eukaryota --- Phenomena and Processes --- Genetics --- Cytology --- Biology --- Health & Biological Sciences --- Génie génétique animal --- Animaux transgéniques --- Animal biotechnology --- Genetic engineering --- Transgenic organisms --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Cells --- Cytologists
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