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Liver Diseases --- Liver --- -Diseases
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Glucuronate formation from UDP-glucuronate is widely to be the result of two consecutive reactions: hydrolysis of UDP-glucuronate to glucuronate-1-P and UMP by a nucleotide pyrophosphatase followed by the dephosphorylation of glucuronate-1-P by a phosphatase. Recent results obtained in this laboratory show that aminopyrine and other xenobiotics stimulate glucuronate production in isolated rat hepatocytes. The purpose of the present study was to identify the target enzyme for this regulation.
Our results show that rat liver microsomes contain an enzyme catalyzing the direct hydrolysis of UDP-glucuronate to glucuronate. We managed to separate this enzyme from nucleotide pyrophosphatase by anion-exchange chromatography and both were characterized. We found that the pyrophosphatase is not specific for UDP-glucuronate; it most probably corresponds to nucleotide pyrophosphatases associated with the plasma membrane. By contrast the newly identified UDP-glucuronidase appears to be quite specific for UDP-glucuronate and is stimulated by UDP-GlcNAc, suggesting that it is associated with the endoplasmic reticulum. It’s sensitively to several detergents suggests that is an integral protein. Interestingly, its activity is inhibited in the presence of CoASH and ATP and can then be stimulated by several xenobiotics that have been shown to increase glucuronate formation in isolated rat hepatocytes. The effect CoASH and ATP is most probably mediated through the formation of acyl-CoAs.
These results argue against the implication of a pyrophosphatase in glucuronate formation in liver microsomes and suggest that this formation proceeds as a single reaction consisting of the f-direct hydrolysis of UDP-glucuronate to UDP and glucuronate. This reaction seems to be affected positively by xenobiotics and negatively by acyl-CoAs Il est classiquement admis que la formation de glucuronate à partir d’UDP-glucuronate se fait via deux réactions successives : l’hydrolyse d’UDP-glucuronate en glucuronate-1-P et UMP par la nucléotide pyrophosphatase suivie de la déphosphorylation du glucuronate-1-P par une phosphatase. Des résultats obtenus récemment dans ce laboratoire indiquent que l’aminopyrine ainsi que d’autres xénobiotiques stimulent la production de glucuronate dans les hépatocytes isolés de foie de rat. L’objectif de ce travail était l’identification de l’enzyme stimulée par ces xénobiotiques.
Les résultats obtenus suggèrent l’existence d’une enzyme qui hydrolyse directement l’UDP-glucuronate en UDP et glucuronate dans des microsomes de foie de rat. On a séparé cette enzyme de la nucléotide pyrophosphatase par chromatographie échangeuse d’anions et on a ensuite caractérisé ces deux enzymes. Nos expériences montrent que la pyrophosphatase n’est pas spécifique pour l’UDP-glucuronate et qu’elle fait probablement partie de la famille des nucléotides pyrophosphatases associées à la membrane plasmique. Par contre, l’UDP-glucuronidase nouvellement identifiée semble être spécifique pour l’UDP-glucuronate. Le fait qu’elle soit stimulée par l’UDP-GIcNAc suggère qu’elle est associée à la membrane du réticulum endoplasmique et sa sensibilité aux détergents indique qu’elle est vraisemblablement intégrée dans la membrane. Par ailleurs, l’activité UDP-glucuronidase est inhibée en présence de CoASH et d’ATP, et cette inhibition est antagonisée par les xénobiotiques qui stimulent la formation de glucuronate dans des hépacytes isolés de foie de rat. Les effets du CoASH et de l’ATP sont vraisemblablement médiés par des dérives acyl-CoA.
Ces résultats plaident contre l’implication d’une pyrophosphatase dans la formation de glucuronate dans les microsomes de foie et suggèrent que cette formation se fait par hydrolyse directe de l’UDP-glucuronate en UDP et glucuronate. Cette réaction semble être affectée positivement par des xénobiotiques et négativement par des acyl-CoAS
Glucuronates --- Liver --- Rats
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Hepatitis --- Liver Diseases. --- Gastrointestinal Diseases. --- Hepatitis. --- Functional Gastrointestinal Disorders --- Gastrointestinal Disorders, Functional --- Cholera Infantum --- Disease, Gastrointestinal --- Diseases, Gastrointestinal --- Disorder, Functional Gastrointestinal --- Disorders, Functional Gastrointestinal --- Functional Gastrointestinal Disorder --- Gastrointestinal Disease --- Gastrointestinal Disorder, Functional --- Infantum, Cholera --- Liver Dysfunction --- Disease, Liver --- Diseases, Liver --- Dysfunction, Liver --- Dysfunctions, Liver --- Liver Disease --- Liver Dysfunctions --- Liver --- Inflammation --- Liver Diseases --- Gastrointestinal Diseases --- Gastroenterology --- Diseases --- Gastrointestinal Disorders --- Gastrointestinal Disorder
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Drugs --- Hepatotoxicology. --- Liver diseases --- Liver --- Liver --- Pharmaceutical Preparations --- Side effects. --- chemically induced. --- Effect of drugs on. --- drug effects. --- adverse effects.
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Extracellular Matrix of the Liver addresses the basic science of the extracellular matrix and discussesnew strategies for the treatment of cirrhosis of the liver, with a primary focus on possible gene therapyapproaches.The chapters are divided into six sections as follows:* Basic Science of Extracellular Matrix* Cells Responsible for Extracellular Matrix Production* Activation Mechanism of Hepatic Cells and Signal Transduction* Basic Science for Extracellular Matrix Metabolism including Enzymes and their Inhibitors* Matrix Mettaloproteinases
Liver --- Extracellular matrix. --- Cirrhosis --- Treatment. --- Gene therapy.
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Liver Neoplasms --- Diagnostic Imaging --- Liver --- diagnosis --- methods --- Cancer --- Diagnosis --- radiography --- Geneeskundige beeldvorming --- Hepatologie --- Tumor --- Imagerie médicale --- Hépatologie --- Tumeur --- diagnosis. --- methods. --- Liver Neoplasms - radiography
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Liver --- Diagnosis, Differential. --- Magnetic resonance imaging. --- Diseases --- Diagnosis.
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Kidney --- Liver --- Pancreas --- Kidney Transplantation --- Liver Transplantation --- Pancreas Transplantation --- radiography --- Geneeskundige beeldvorming --- Orgaantransplantatie --- Nefrologie --- Hepatologie --- Imagerie médicale --- Transplantation d'organes --- Néphrologie --- Hépatologie --- Pancréas --- Kidney - radiography --- Liver - radiography --- Pancreas - radiography
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The purpose of this volume is to provide a short, didactic handbook for those clinicians (medical, surgical, nursing and others) who are involved in the care and management of people who may, are or have undergone liver transplantation.
Advertising agencies-- Forecasting-- Statistics. --- Liver Transplantation. --- Digestive System Surgical Procedures --- Tissue Transplantation --- Organ Transplantation --- Digestive System Diseases --- Liver Transplantation --- Surgical Procedures, Operative --- Liver Diseases --- Diseases --- Transplantation --- Transplantations --- Transplantation Immunology --- Transplantation Conditioning --- Liver Dysfunction --- Disease, Liver --- Diseases, Liver --- Dysfunction, Liver --- Dysfunctions, Liver --- Liver Disease --- Liver Dysfunctions --- Ghost Surgery --- Operative Procedures --- Operative Surgical Procedure --- Operative Surgical Procedures --- Procedure, Operative Surgical --- Procedures, Operative Surgical --- Surgery, Ghost --- Surgical Procedure, Operative --- Surgical Procedures --- Operative Procedure --- Procedure, Operative --- Procedure, Surgical --- Procedures, Operative --- Procedures, Surgical --- Surgical Procedure --- General Surgery --- Grafting, Liver --- Hepatic Transplantation --- Liver Transplant --- Transplantation, Hepatic --- Transplantation, Liver --- Hepatic Transplantations --- Liver Grafting --- Liver Transplantations --- Liver Transplants --- Transplant, Liver --- Liver --- Digestive System Disorders --- Hepatobiliary Diseases --- Hepatobiliary Disorders --- Digestive System Disease --- Digestive System Disorder --- Hepatobiliary Disease --- Hepatobiliary Disorder --- System Disorders, Digestive --- Grafting, Organ --- Transplantation, Organ --- Graftings, Organ --- Organ Grafting --- Organ Graftings --- Organ Transplantations --- Transplantations, Organ --- Grafting, Tissue --- Transplantation, Tissue --- Tissue Grafting --- Tissues --- Digestive System Surgical Procedure --- Gastrointestinal Surgical Procedure --- Procedure, Digestive System Surgical --- Procedure, Gastrointestinal Surgical --- Procedures, Digestive System Surgical --- Procedures, Gastrointestinal Surgical --- Surgical Procedure, Digestive System --- Surgical Procedure, Gastrointestinal --- Surgical Procedures, Digestive System --- Surgical Procedures, Gastrointestinal --- Gastrointestinal Surgical Procedures --- Digestive System --- transplantation --- surgery --- Transplantation. --- Hepatic transplantation
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Aprotinin --- Liver Transplantation --- Graft Survival --- Hemostatics --- administration & dosage. --- methods. --- drug effects. --- Theses --- administration & dosage --- methods --- drug effects
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