Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Exons (Genetics). --- Genetic recombination. --- Split genes.

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Directed protein evolution-a powerful technique for the discovery of new enzymes and therapeutic proteins-has matured to the point that it is now the centerpiece of enzyme engineering, where it has catalyzed the development of numerous experimental methods. In Directed Evolution Library Creation: Methods and Protocols, seasoned practitioners from many leading laboratories share their best experimental protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologous recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Taken together, Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods capture for newcomers and more experienced investigators alike all the key methods for using directed protein evolution to better understand protein structure-function relationships, to discover new enzymes and therapeutic proteins, and to design new assays suitable for specific applications.

Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Gene libraries --- Genetic engineering --- Molecular evolution --- Microbial mutation --- Combinatorial chemistry --- Genetic recombination

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Genetic engineering --- Genes --- Law and legislation --- Patents. --- Patents --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Units of heredity --- Units of inheritance --- Heredity --- Molecular genetics --- DNA --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Genetic engineering - Law and legislation - Great Britain. --- Genes - Great Britain - Patents. --- DROIT MEDICAL --- BIOETHIQUE --- EXPERIMENTATION MEDICALE --- THERAPIE GENETIQUE --- OGM

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

The authors conclude that while IGM cannot be carried out safely and responsibly on humans utilizing current methods, it is important to begin public discussion now to determine whether, and if so how, to proceed.

Public Policy. --- Bioethical Issues. --- Genetic Engineering. --- Medical genetics. --- Genetic engineering --- Genetic engineering. --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Government policy --- Moral and ethical aspects. --- Genetic aspects

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

The Human Genome Project, discoveries in molecular biology and new reproductive technologies have advanced our understanding of how genetic science may be used to treat persons with genetic disorders. Greater knowledge may also make possible genetic interventions to "enhance" normal human characteristics, such as height, hair or eye colour, strength, or memory, as well as the transmittal of such modifications to future generations. The prospect of inheritable genetic modifications, or IGMs, whether for therapeutic or enhancement purposes, raises complex scientific, ethical and regulatory issues. This volume presents 20 essays by physicians, scientists, philosophers, theologians, lawyers and policy analysts addressing these issues from diverse perspectives. In three sections, the authors discuss the short- and long-term scientific feasibility of IGM technology; ethical and religious issues related to safety, justice, morality, reproductive rights, and enhancement; and regulatory issues including the necessity of public input and oversight and the influence of commercialization. Their goal is to open a dialogue engaging not only scholars and scientists but also government officials and concerned citizens. The authors conclude that while IGM cannot be carried out safely and responsibly on humans utilizing current methods, it is important to begin public discussion now to determine whether, and if so how, to proceed. The prospect of inheritable genetic modifications for therapeutic or enhancement purposes raises complex scientific, ethical and regulatory issues. These 20 essays, by physicians, lawyers, theologians and others, address these issues from a variety of perspectives.

Bioethical Issues. --- Genetic Engineering. --- Genetic engineering --- Genetic engineering. --- Medical genetics. --- Public policy. --- Government policy --- Moral and ethical aspects. --- menselijk genoom --- gentherapie --- ethiek (ethische aspecten) --- godsdienst (religie, religieuze aspecten) --- génome humain --- thérapie génique --- ethique (aspects ethiques) --- religion (aspects religieux) --- Medical genetics --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Moral and ethical aspects --- Genetic aspects