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Dieses erfolgreiche Kurzlehrbuch der Immunologie führt ein in die molekularen, zellbiologischen und physiologischen Grundlagen der immunologischen Abwehrmechanismen des menschlichen und tierischen Organismus bei Kontakt mit infektiösen Erregern sowie in die Methoden ihrer Untersuchung. Auch immunpathologische Krankheitszustände sowie Interventionsmöglichkeiten werden erörtert, so sie zum Verständnis relevant sind. Bei Studierenden aller biologischen, biochemischen, pharmazeutischen, medizinischen, veterinärmedizinischen Fachrichtungen, aber auch bei Ärzten, Apothekern oder Lehrern in der Fortbildung sowie interessierten Laien soll das Buch das Interesse für die Immunologie wecken und das Verstehen der Zusammenhänge erleichtern. Fragen führen durch das Buch und der Leser wird animiert, sein Wissen selbständig weiter zu vertiefen. MEMO-Boxen fassen Wichtiges zusammen und im Anhang stehen "Fakten und Zahlen".

Immunology. --- Infectious diseases. --- Biochemistry. --- Bacteriology. --- Internal medicine. --- Microbiology. --- Infectious Diseases. --- Biochemistry, general. --- Internal Medicine.

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Das Fachlexikon Toxikologie gibt den auf dem Gebiet des Umweltschutzes, der Arbeitshygiene und Toxikologie tätigen Wissenschaftlern und Praktikern ebenso ein Hilfsmittel in die Hand wie den mit dem Transport gefährlicher Güter oder den mit der schadlosen Beseitigung toxischer Abprodukte Beschäftigten. Daneben wendet sich das Fachlexikon auch an die Mitarbeiter von Feuerwehr, Polizei, Rettungsdiensten, Zoll und anderen Kontrollbehörden sowie an Lehrende und Studierende naturwissenschaftlicher Disziplinen, die nach einem kompakten und zugleich kompetenten Ratgeber suchen.

Pharmacology. --- Pharmacy. --- Emergency medicine. --- Forensic medicine. --- Biochemistry. --- Pharmacology/Toxicology. --- Emergency Medicine. --- Forensic Medicine. --- Biochemistry, general. --- Toxicology --- German.

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Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers examine these cutting edge proteomics methodologies, explore their application to a wide range of biological materials, and look at how these developments have helped standardize the design, execution, and analysis of proteomics experiments. Chapters reflect key steps in 2-DE experiments, including sample preparation, staining, post-translational modification, spot identification, and bioinformatics. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and reader-friendly, Two-Dimensional Electrophoresis Protocols encourages newcomers to apply powerful 2-DE techniques to their own research, while also providing current and essential information for seasoned scientists.

Biochemistry. --- Proteomics. --- Electrophoresis. --- Biochemistry, general. --- Protein Science. --- Cataphoresis --- Electrochemistry --- Phase partition --- Molecular biology --- Proteins --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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Since the development of the concept of "proteomics," great progress has granted deeper insight into the cellular network, but the enormous range of protein abundance, dynamics, and interactions leaves many further challenges in the field. In Proteomics: Methods and Protocols, experts in proteomic research have contributed detailed techniques, which present an overview of the contemporary challenges and possibilities in the various areas of proteomics, as well as cutting-edge protocols that provide concrete examples for successful proteome analysis. Written in the clear and concise Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Proteomics: Methods and Protocols will serve as a valuable reference book for laboratories around the world and raise interest in the complex and vital field of proteomics research.

Biochemistry. --- Proteomics. --- Protein Science. --- Biochemistry, general. --- Molecular biology --- Proteins --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.

Biochemistry. --- Biochemistry, general. --- Protein Science. --- Protein Structure. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Business, Economy and Management --- Organizational Psychology