Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

In recent years, a new understanding of cell-penetrating peptides has emerged, helping researchers to expand beyond a number of long-held dogmas. In Cell-Penetrating Peptides: Methods and Protocols, expert researchers explore the latest information on cell-penetrating peptides (CPPs), providing insight into the most important and contemporary areas of CPP research. Chapters address the historical background of CPP studies, provide an overview of the growing field of research, investigate methods for testing CPP mechanisms, present a summary of methods that attempt to use properties of CPPs to study biochemical intracellular mechanisms of interaction and signal transduction, and include new ideas for turning CPP-based strategies into drugs. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and current, Cell-Penetrating Peptides: Methods and Protocols is an essential guide that allows researchers to study intracellular mechanisms in new ways, and to promote the future development of novel drugs.

Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.

Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Fungi --- Mycology --- Cell biology --- Treatises

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Poly (ADP-ribose) Polymerases (PARPs) are abundant and ubiquitous proteins that regulate crucial processes of the cell cycle, DNA repair, genomic stability, and transcriptional regulation.  Being involved in basic cell functions, PARPs mediate rapid responses to such environmental factors as stress, infection, nutrition and hormonal signals. Whereas PARP inhibitors can suppress tumor growth and proliferation in certain breast, ovarian, and prostate cancers, understanding how PARP controls cellular functions is essential for the development of novel cancer treatments strategies. Divided into three convenient sections, Poly(ADP-Ribose) Polymerase: Methods and Protocols aims to explain how PARP proteins act within the normal development of an organism as well as in pathogenic conditions, seeks to advance the knowledge of developmental pathways regulation, and endeavors to facilitate the development of new therapeutic drugs and methods to target PARP-dependent processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Poly(ADP-Ribose) Polymerase: Methods and Protocols serves as an ideal guide to scientists who wish to continue exploring this exciting and progressive research field.  .

Cytology. --- Proteomics. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Molecular biology --- Proteins

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Les biopsies ganglionnaires sont des échantillons précieux qui ne sont pas rares dans les laboratoires d’hématologie. Vu leur importance diagnostique, il est nécessaire de développer au mieux les techniques disponibles dans un laboratoire spécialisé afin d’améliorer la précision dans le diagnostique ainsi que le temps de réponse de l’analyse. La cytologie sur empreinte, technique courante de première intention, apporte une information importante sur la morphologie cellulaire des différents éléments qui la composent mais pas sur sa structure. Cependant, sa lecture n’est pas des plu simple, l’observateur doit présenter une certaine expérience dans ce domaine. De plus, l’empreinte devra libérer de son suc les cellules d’intérêt qui permettront de poser le diagnostic. A côté de cette technique, nous aurons l’histologie réservée à l’anatomie pathologique, la cytogénétique ainsi que la biologie moléculaire. Elles donneront le diagnostic définitif mais dans un délai beaucoup plus long. C’est donc à la demande des cliniciens que nous avons développé en cytométrie en flux une technique en trois tubes permettant d’orienter et d’améliorer le diagnostic à partir des différents matériels de biopsie ou d’aspiration à l’aiguille fine. Le but premier de cette méthode est de permettre de faire la différence entre ganglion bénin ou malin ainsi que de diagnostiquer le type de pathologie ganglionnaire : lymphomes hodgkinien ou non-hodgkiniens, métastases d’une carcinome et autres

Sentinel Lymph Node Biopsy --- Cell Biology --- Ganglion Cysts --- Image Cytometry