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The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry. Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.
Human genetics. --- Genetic engineering. --- Human Genetics. --- Genetic Engineering. --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology
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With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner. Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels. In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research.
Biochemistry. --- Genetic engineering. --- Protein Science. --- Genetic Engineering. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Composition
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Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In Strain Engineering: Methods and Protocols, powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. These methods are particularly powerful when utilized to manipulate microbes for which sequenced and annotated genomes are available. Collectively, these methods systematically introduce genome alterations in a precise manner, allowing the creation of novel strains carrying only desired genome alterations. In the first section, E. coli-based bacterial strain engineering strategies are reviewed, while the second section presents analogous microbial engineering strategies for eukaryotic cells using the yeast Saccharomyces cerevisiae as a model. The third section covers examples of the proliferative adaptations of these base technologies to strain engineer industrially important prokaryotic or eukaryotic microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Strain Engineering: Methods and Protocols serves as an ideal guide to scientists in academia, pharmaceutical science, and biotechnology who perform microbial strain engineering.
Human genetics. --- Genetic engineering. --- Human Genetics. --- Genetic Engineering. --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology
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Humans have been modifying plants and animals for millennia. The dawn of molecular genetics, however, has kindled intense public scrutiny and controversy. Crops, and the food products which include them, have dominated molecular modification in agriculture. Organisations have made unsubstantiated claims and scare mongering is common. In this textbook Paul Thompson presents a clear account of the significant issues - identifying harms and benefits, analysing and managing risk - which lie beneath the cacophony of public controversy. His comprehensive analysis looks especially at genetically modified organisms, and includes an explanation of the scientific background, an analysis of ideological objections, a discussion of legal and ethical concerns, a suggested alternative - organic agriculture - and an examination of the controversy's impact on sub-Saharan African countries. His book will be of interest to students and other readers in philosophy, biology, biotechnology and public policy.
Agricultural biotechnology --- Agricultural biotechnology. --- Genetic engineering --- Genetic engineering. --- Moral and ethical aspects. --- Philosophy. --- Social aspects. --- Arts and Humanities --- Philosophy --- Agro-biotechnology --- Biotechnology --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Transgenic organisms
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Biology --- Computer. Automation --- Biocomputers --- 575.08:577.21 --- 576.3 --- 577.21 --- 577.21 Molecular mechanism of coding, storage and realization of inheritance information. Molecular genetics. Molecular biology of the gene --- Molecular mechanism of coding, storage and realization of inheritance information. Molecular genetics. Molecular biology of the gene --- 576.3 General cytology. The cell as a biological system. General plan, chemical composition, physicochemical and molecular properties --- General cytology. The cell as a biological system. General plan, chemical composition, physicochemical and molecular properties --- 575.08:577.21 Genetic engineering, genetic manipulation, recombinant DNA --- Genetic engineering, genetic manipulation, recombinant DNA --- Biochemical computers --- Bioelectronic computers --- Biomechanical computers --- Computers --- Conscious automata
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With an increasing human population and a decreasing amount of arable land, creative improvements in agriculture will be a necessity in the coming decades to maintain or improve the standard of living. In Plant Chromosome Engineering: Methods and Protocols, expert researchers present techniques for the modification of crops and other plant species in order to achieve the goal of developing the much needed novel approaches to the production of food, feed, fuel, fiber, and pharmaceuticals. This volume examines vital topics such as transformation procedures, chromosome painting, production of engineered minichromosomes, gene targeting and mutagenesis, site specific integration, gene silencing, protein expression, chromosome sorting and analysis, protocols for generating chromosomal rearrangements, enhancer trapping, and means of studying chromosomes in vivo. As a part of the highly successful Methods in Molecular Biology™ series, the methodological chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and professional tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Chromosome Engineering: Methods and Protocols highlights the spectrum of tools currently available for modifying plant genomes and chromosomes and provides the foundation for crucial future developments.
Botany. --- Plant genetics. --- Genetic engineering. --- Plant Sciences. --- Plant Genetics and Genomics. --- Genetic Engineering. --- Plants --- Genetics --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Floristic botany
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Focusing on four major issues, the authors examine the ways in which genetic technologies are shaping criminal justice practices, how policies on reproductive technologies have shifted in response to biotechnologies, the debates surrounding the patenting of higher life forms, and the Canadian (and global) response to bioterrorism. Regulatory strategies in government and the courts are continually evolving and are affected by changing public perceptions of scientific knowledge. The legal and cultural shifts outlined in Becoming Biosubjects call into question what it means to be a Canadian, a citizen, and a human being."--Pub. desc "Becoming Biosubjects examines the ways in which the Canadian government, media, courts, and everyday Canadians are making sense of the challenges being posed by biotechnologies. The authors argue that the human body is now being understood as something that is fluid and without fixed meaning. This has significant implications both for how we understand ourselves and how we see our relationships with other forms of life.
Criminal justice, Administration of --- Forensic genetics --- Human body. --- Genetic engineering --- Biotechnology --- Reproductive technology --- Bioterrorism --- Bio-terrorism --- Biological terrorism --- Terrorism --- Chemical engineering --- ART (Assisted reproductive technology) --- Assisted reproduction --- Assisted reproductive technology --- Reproduction --- Reproductive techniques --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Transgenic organisms --- Body, Human --- Human beings --- Body image --- Human anatomy --- Human physiology --- Mind and body --- Technique. --- Social aspects --- Government policy --- Prevention. --- Law and legislation --- Technological innovations --- Human body --- Technique --- Prevention --- Biotechnology - Social aspects - Canada --- Genetic engineering - Social aspects - Canada --- Forensic genetics - Technique --- Criminal justice, Administration of - Canada --- Reproductive technology - Government policy - Canada --- Biotechnology - Canada - Patents --- Bioterrorism - Canada - Prevention --- Bioterrorism - Prevention --- Kanada. --- Canada. --- Canada --- Canada (Province) --- Canadae --- Ceanada --- Chanada --- Chanadey --- Dominio del Canad --- Dominion of Canada --- Jianada --- Kʻaenada --- Kanada --- Ḳanadah --- Kanadaja --- Kanadas --- Ḳanade --- Kanado --- Kanak --- Province of Canada --- Republica de Canad --- Yn Chanadey --- Dominio del Canadá --- Kaineḍā --- Kanakā --- Republica de Canadá
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Safe or Not Safe aims to present a comprehensive picture of food-related risk issues, as observed in daily life. Included are numerous real-life examples showing how scientists measure, assess, evaluate and manage potential risks, and how this helps us to decide how to manage our food chain and ultimately the food we eat. The book is divided into four interrelated chapters. Chapter one deals with risk: Risk examples are given from our daily life, illustrating what we need to know about risks and how to manage them. Chapter two addresses the risks to biodiversity. Chapter three investigates the risks chemical contaminants pose to our health through the food chain. The final chapter reviews the last three decades of creating genetically engineered food, focusing not only on the science behind the technology, but also on issues, such as consumer choice and rights, politics, product usefulness, and the availability of alternatives. Aimed especially at food science professionals and educators, undergraduate and graduate students, health professionals, and an interested general public will also benefit greatly from this resource.
Food -- Biotechnology -- Safety measures. --- Food -- Safety measures. --- Food contamination -- Prevention. --- Food industry and trade -- Safety measures. --- Food spoilage -- Prevention. --- Food --- Environmental risk assessment --- Health & Biological Sciences --- Business & Economics --- Agricultural Economics --- Diet & Clinical Nutrition --- Biomedical Engineering --- Safety measures --- Environmental risk assessment. --- Safety measures. --- Chemistry. --- Genetic engineering. --- Nutrition. --- Quality control. --- Reliability. --- Industrial safety. --- Food Science. --- Genetic Engineering. --- Quality Control, Reliability, Safety and Risk. --- Biotechnology. --- Risk assessment --- Precautionary principle --- Food science. --- System safety. --- Safety, System --- Safety of systems --- Systems safety --- Accidents --- Industrial safety --- Systems engineering --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Alimentation --- Nutrition --- Health --- Physiology --- Diet --- Dietetics --- Digestion --- Food habits --- Malnutrition --- Science --- Prevention --- Health aspects --- Food—Biotechnology. --- Nutrition . --- Industrial accidents --- Industries --- Job safety --- Occupational hazards, Prevention of --- Occupational health and safety --- Occupational safety and health --- Prevention of industrial accidents --- Prevention of occupational hazards --- Safety, Industrial --- Safety engineering --- Safety of workers --- System safety --- Dependability --- Trustworthiness --- Conduct of life --- Factory management --- Industrial engineering --- Reliability (Engineering) --- Sampling (Statistics) --- Standardization --- Quality assurance --- Quality of products
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The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenic Mouse Methods and Protocols, Second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.
Transgenic mice --- Animals, Genetically Modified --- Investigative Techniques --- Mice --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Murinae --- Animal Population Groups --- Animals --- Muridae --- Eukaryota --- Rodentia --- Mammals --- Organisms --- Vertebrates --- Chordata --- Mice, Transgenic --- Genetic Techniques --- Biology --- Health & Biological Sciences --- Genetics --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Founder Mice, Transgenic --- Mouse, Founder, Transgenic --- Mouse, Transgenic --- Transgenic Mice --- Mice, Transgenic Founder --- Transgenic Founder Mice --- Transgenic Mouse --- Chordates --- Chordate --- Vertebrate --- Mammalia --- Mammal --- Rodents --- Beavers --- Capybaras --- Castor Beaver --- Dipodidae --- Hydrochaeris --- Jerboas --- Beaver --- Capybara --- Hydrochaeri --- Jerboa --- Rodent --- Rodentias --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Murids --- Murid --- Animalia --- Animal --- Metazoa --- Animal Population Group --- Population Group, Animal --- Population Groups, Animal --- Acomys --- Apodemus --- Bandicoot Rats --- Bandicota --- Mastomys --- Praomys --- Rats, Bandicoot --- Bandicoot Rat --- Bandicotas --- Rat, Bandicoot --- Mice, Laboratory --- Mouse --- Mouse, House --- Mouse, Laboratory --- Mouse, Swiss --- Mus domesticus --- Mus musculus domesticus --- Swiss Mice --- Mice, House --- Mus --- Mus musculus --- House Mice --- House Mouse --- Laboratory Mice --- Laboratory Mouse --- Mice, Swiss --- Swiss Mouse --- domesticus, Mus musculus --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Founder Animals, Transgenic --- GMO Animals --- Genetically Engineered Animals --- Animals, Transgenic --- Genetically Modified Animals --- Transgenic Animals --- Animal, GMO --- Animal, Genetically Engineered --- Animal, Genetically Modified --- Animal, Transgenic --- Animal, Transgenic Founder --- Animals, GMO --- Animals, Genetically Engineered --- Animals, Transgenic Founder --- Engineered Animal, Genetically --- Engineered Animals, Genetically --- Founder Animal, Transgenic --- GMO Animal --- Genetically Engineered Animal --- Genetically Modified Animal --- Modified Animal, Genetically --- Modified Animals, Genetically --- Transgenic Animal --- Transgenic Founder Animal --- Transgenic Founder Animals --- methods --- Animal genetics. --- Genetic engineering. --- Medical genetics. --- Animal Genetics and Genomics. --- Genetic Engineering. --- Gene Function. --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Genetic aspects --- Transgenic mice - Laboratory manuals
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This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.
Transgenic animals --- Genetic engineering --- Genetic Engineering --- Animals, Genetically Modified --- Methodology --- methods --- Investigative Techniques --- Organisms, Genetically Modified --- Publication Formats --- Animal Population Groups --- Genetic Techniques --- Animals --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Organism Forms --- Publication Characteristics --- Eukaryota --- Organisms --- Laboratory Manuals --- Methods --- Biology --- Health & Biological Sciences --- Genetics --- Engineering, Genetic --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- Founder Animals, Transgenic --- GMO Animals --- Genetically Engineered Animals --- Animals, Transgenic --- Genetically Modified Animals --- Transgenic Animals --- Animal, GMO --- Animal, Genetically Engineered --- Animal, Genetically Modified --- Animal, Transgenic --- Animal, Transgenic Founder --- Animals, GMO --- Animals, Genetically Engineered --- Animals, Transgenic Founder --- Engineered Animal, Genetically --- Engineered Animals, Genetically --- Founder Animal, Transgenic --- GMO Animal --- Genetically Engineered Animal --- Genetically Modified Animal --- Modified Animal, Genetically --- Modified Animals, Genetically --- Transgenic Animal --- Transgenic Founder Animal --- Transgenic Founder Animals --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Animalia --- Animal --- Metazoa --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Animal Population Group --- Population Group, Animal --- Population Groups, Animal --- GMO Organisms --- Genetically Engineered Organisms --- Genetically Modified Organisms --- Organisms, Transgenic --- Transgenic Organisms --- Engineered Organism, Genetically --- Engineered Organisms, Genetically --- GMO Organism --- Genetically Engineered Organism --- Genetically Modified Organism --- Modified Organism, Genetically --- Modified Organisms, Genetically --- Organism, GMO --- Organism, Genetically Engineered --- Organism, Genetically Modified --- Organism, Transgenic --- Organisms, GMO --- Organisms, Genetically Engineered --- Transgenic Organism --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Designed genetic change --- Gene splicing --- Genetic intervention --- Genetic surgery --- Life sciences. --- Animal genetics. --- Transgenic organisms. --- Life Sciences. --- Animal Genetics and Genomics. --- Transgenics. --- Genetically engineered organisms --- Genetically modified organisms --- GEOs (Genetically engineered organisms) --- GMOs (Genetically modified organisms) --- Biosciences --- Sciences, Life --- Science
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