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In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of bio

Enzymes. --- Enzymology

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The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues.  In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei, adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, RNA and DNA Editing: Methods and Protocols seeks to inspire the further development of these vital and powerful techniques.

Human genetics. --- Enzymes. --- Human Genetics. --- Enzymology. --- Biocatalysts --- Ferments --- Soluble ferments --- Catalysts --- Proteins --- Enzymology --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology

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Today, activation endoproteolysis of secretory proteins is recognized as a fundamental biological mechanism of spatial and temporal regulation of protein activity as well as of diversification of protein functions.  In Proprotein Convertases, experts in the field examine detailed methods involving proprotein convertases, the enzymes mediating this endoproteolysis, which reside within or cycle between the various compartments of the secretory pathway.  Providing a timely assessment of impact of activation/inactivation endoproteolysis in the secretory pathway, the volume offers a broader perspective on the biochemistry of the PCSKs (proprotein convertases, subtilisin/kexin-type) by exploring structural and functional analogies with bacterial subtilisin and on the enzymology of endoproteolysis itself by describing the involvement in the process of non-PCSK-type such as cathepsin L.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Meticulous and up-to-date, Proprotein Convertases represents an instructive and useful reference book for all scientists interested in endoproteolytic activation and/or inactivation of secretory proproteins through limited proteolysis, for experts in the field and newcomers to it as well.

Biochemistry. --- Enzymes. --- Biochemistry, general. --- Enzymology. --- Biocatalysts --- Ferments --- Soluble ferments --- Catalysts --- Proteins --- Enzymology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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Organocatalysis has emerged as one of the hot topics in organic chemistry in recent years, as confirmed by the rapid-growing interest that researchers have shown in this field. Enantioselective Organocatalyzed Reaction Volume I and II provides a critical review of the state-of-the-art developments in Organocatalysis, with a special focus on the asymmetric transformation that it enables. These volumes, written by leading experts in the field, discuss a broad range of synthetic transformations and offer an up-to-date coverage of organocatalyzed reactions such as: ¢ Conjugate addition; ¢ Aldol addition; ¢ Oxidation; ¢ Reduction; ¢ Cycloaddition; ¢ Mannich reaction; ¢ Asymmetric f untionalization; ¢ Enantioselective  protonation; ¢ General aspects of asymmetric cyclization; ¢ Asymmetric desymmetrization processes. By presenting a complete panorama of the use of organocatalysts in organic reaction, Enantioselective Organocatalyzed Reaction will help the global audience of scientists engaged in this area of research to develop new catalysts as well as new fields of applications in organic synthesis.

Chemical thermodynamics --- Organic chemistry --- Enzymology --- Biotechnology --- katalyse --- thermodynamica --- organische chemie --- biochemie --- biotechnologie --- enzymen

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The importance of solid base catalysts has come to be recognized for their environmentally benign qualities, and much significant progress has been made over the past two decades in catalytic materials and solid base-catalyzed reactions. The book is focused on the solid base. Because of the advantages over liquid bases, the use of solid base catalysts in organic synthesis is expanding. Solid bases are easier to dispose than liquid bases, separation and recovery of products, catalysts and solvents are less difficult, and they are non-corrosive. Furthermore, base-catalyzed reactions can be performed without using solvents and even in the gas phase, opening up more possibilities for discovering novel reaction systems. Using numerous examples, the present volume describes the remarkable role solid base catalysis can play, given the ever increasing worldwide importance of "green" chemistry. The reader will obtain an overall view of solid base catalysis and gain insight into the versatility of the reactions to which solid base catalysts can be utilized. The concept and significance of solid base catalysis are discussed, followed by descriptions of various methods for the characterization of solid bases, including spectroscopic methods and test reactions. The preparation and properties of base materials are presented in detail, with the two final chapters devoted to surveying the variety of reactions catalyzed by solid bases.

Chemical thermodynamics --- Physicochemistry --- Enzymology --- Engineering sciences. Technology --- Biotechnology --- katalyse --- katalysatoren --- thermodynamica --- biochemie --- biotechnologie --- ingenieurswetenschappen --- fysicochemie --- enzymen