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The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenic Mouse Methods and Protocols, Second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.

Transgenic mice --- Animals, Genetically Modified --- Investigative Techniques --- Mice --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Murinae --- Animal Population Groups --- Animals --- Muridae --- Eukaryota --- Rodentia --- Mammals --- Organisms --- Vertebrates --- Chordata --- Mice, Transgenic --- Genetic Techniques --- Biology --- Health & Biological Sciences --- Genetics --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Founder Mice, Transgenic --- Mouse, Founder, Transgenic --- Mouse, Transgenic --- Transgenic Mice --- Mice, Transgenic Founder --- Transgenic Founder Mice --- Transgenic Mouse --- Chordates --- Chordate --- Vertebrate --- Mammalia --- Mammal --- Rodents --- Beavers --- Capybaras --- Castor Beaver --- Dipodidae --- Hydrochaeris --- Jerboas --- Beaver --- Capybara --- Hydrochaeri --- Jerboa --- Rodent --- Rodentias --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Murids --- Murid --- Animalia --- Animal --- Metazoa --- Animal Population Group --- Population Group, Animal --- Population Groups, Animal --- Acomys --- Apodemus --- Bandicoot Rats --- Bandicota --- Mastomys --- Praomys --- Rats, Bandicoot --- Bandicoot Rat --- Bandicotas --- Rat, Bandicoot --- Mice, Laboratory --- Mouse --- Mouse, House --- Mouse, Laboratory --- Mouse, Swiss --- Mus domesticus --- Mus musculus domesticus --- Swiss Mice --- Mice, House --- Mus --- Mus musculus --- House Mice --- House Mouse --- Laboratory Mice --- Laboratory Mouse --- Mice, Swiss --- Swiss Mouse --- domesticus, Mus musculus --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Founder Animals, Transgenic --- GMO Animals --- Genetically Engineered Animals --- Animals, Transgenic --- Genetically Modified Animals --- Transgenic Animals --- Animal, GMO --- Animal, Genetically Engineered --- Animal, Genetically Modified --- Animal, Transgenic --- Animal, Transgenic Founder --- Animals, GMO --- Animals, Genetically Engineered --- Animals, Transgenic Founder --- Engineered Animal, Genetically --- Engineered Animals, Genetically --- Founder Animal, Transgenic --- GMO Animal --- Genetically Engineered Animal --- Genetically Modified Animal --- Modified Animal, Genetically --- Modified Animals, Genetically --- Transgenic Animal --- Transgenic Founder Animal --- Transgenic Founder Animals --- methods --- Animal genetics. --- Genetic engineering. --- Medical genetics. --- Animal Genetics and Genomics. --- Genetic Engineering. --- Gene Function. --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Genetic aspects --- Transgenic mice - Laboratory manuals

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Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research.

Human histology. Human cytology --- Chemical laboratory practice --- Tissues --- Tissue culture --- Diagnostic immunohistochemistry --- Tissue Embedding --- Genetic Techniques --- Microarray Analysis --- Culture Techniques --- Publication Formats --- Investigative Techniques --- Clinical Laboratory Techniques --- Histocytological Preparation Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Publication Characteristics --- Microchip Analytical Procedures --- Cytological Techniques --- Chemistry Techniques, Analytical --- Histological Techniques --- Gene Expression Profiling --- Laboratory Manuals --- Paraffin Embedding --- Tissue Array Analysis --- Methods --- Tissue Culture Techniques --- Human Anatomy & Physiology --- Biology --- Health & Biological Sciences --- Physiology --- Biophysics --- Analysis --- Embedding, Paraffin --- Gene Expression Monitoring --- Transcriptome Analysis --- Gene Expression Pattern Analysis --- Transcript Expression Analysis --- Transcriptome Profiling --- mRNA Differential Display --- Analyses, Transcript Expression --- Analyses, Transcriptome --- Analysis, Transcript Expression --- Analysis, Transcriptome --- Differential Display, mRNA --- Differential Displays, mRNA --- Display, mRNA Differential --- Displays, mRNA Differential --- Gene Expression Monitorings --- Gene Expression Profilings --- Monitoring, Gene Expression --- Monitorings, Gene Expression --- Profiling, Gene Expression --- Profiling, Transcriptome --- Profilings, Gene Expression --- Profilings, Transcriptome --- Transcript Expression Analyses --- Transcriptome Analyses --- Transcriptome Profilings --- mRNA Differential Displays --- Histologic Technic --- Histologic Technics --- Histologic Technique --- Histologic Techniques --- Histological Technics --- Technic, Histologic --- Technics, Histologic --- Technique, Histologic --- Techniques, Histologic --- Histological Technic --- Histological Technique --- Technic, Histological --- Technics, Histological --- Technique, Histological --- Techniques, Histological --- Histology --- Analytical Chemistry Methods --- Analytical Chemistry Techniques --- Analytical Chemistry Method --- Analytical Chemistry Technique --- Chemistry Method, Analytical --- Chemistry Methods, Analytical --- Chemistry Technique, Analytical --- Method, Analytical Chemistry --- Methods, Analytical Chemistry --- Technique, Analytical Chemistry --- Techniques, Analytical Chemistry --- Cytologic Technics --- Cytological Technic --- Cytological Technics --- Cytological Technique --- Technic, Cytological --- Technics, Cytological --- Technique, Cytological --- Techniques, Cytological --- Cytologic Technic --- Technic, Cytologic --- Technics, Cytologic --- Cell Biology --- Microchip Analysis --- Analyses, Microchip --- Analysis, Microchip --- Analytical Procedure, Microchip --- Analytical Procedures, Microchip --- Microchip Analytical Procedure --- Procedure, Microchip Analytical --- Procedures, Microchip Analytical --- Cytohistologic Preparation Technics --- Cytohistologic Preparation Technique --- Cytohistologic Preparation Techniques --- Cytohistological Preparation Technic --- Cytohistological Preparation Technics --- Cytohistological Preparation Technique --- Histocytologic Preparation Technic --- Histocytologic Preparation Technics --- Histocytologic Preparation Technique --- Histocytologic Preparation Techniques --- Histocytological Preparation Technic --- Histocytological Preparation Technics --- Histocytological Preparation Technique --- Preparation Technic, Cytohistologic --- Preparation Technic, Cytohistological --- Preparation Technic, Histocytologic --- Preparation Technic, Histocytological --- Preparation Technics, Cytohistologic --- Preparation Technics, Cytohistological --- Preparation Technics, Histocytologic --- Preparation Technics, Histocytological --- Preparation Technique, Cytohistologic --- Preparation Technique, Cytohistological --- Preparation Technique, Histocytologic --- Preparation Technique, Histocytological --- Preparation Techniques, Cytohistologic --- Preparation Techniques, Cytohistological --- Preparation Techniques, Histocytologic --- Preparation Techniques, Histocytological --- Technic, Cytohistologic Preparation --- Technic, Cytohistological Preparation --- Technic, Histocytologic Preparation --- Technic, Histocytological Preparation --- Technics, Cytohistologic Preparation --- Technics, Cytohistological Preparation --- Technics, Histocytologic Preparation --- Technics, Histocytological Preparation --- Technique, Cytohistologic Preparation --- Technique, Cytohistological Preparation --- Technique, Histocytologic Preparation --- Technique, Histocytological Preparation --- Techniques, Cytohistologic Preparation --- Techniques, Cytohistological Preparation --- Techniques, Histocytologic Preparation --- Techniques, Histocytological Preparation --- Cytohistological Preparation Techniques --- Cytohistologic Preparation Technic --- Diagnoses and Laboratory Examinations --- Diagnosis, Laboratory --- Laboratory Diagnosis --- Laboratory Examinations and Diagnoses --- Laboratory Techniques, Clinical --- Clinical Laboratory Technique --- Diagnoses, Laboratory --- Laboratory Diagnoses --- Laboratory Technique, Clinical --- Technique, Clinical Laboratory --- Techniques, Clinical Laboratory --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Culture Technique --- Technique, Culture --- Techniques, Culture --- Microarray Analytical Devices --- Microarray Microchips --- Nanoarray Analytical Devices --- Analysis, Microarray --- Analytical Device, Microarray --- Analytical Device, Nanoarray --- Analytical Devices, Microarray --- Analytical Devices, Nanoarray --- Device, Microarray Analytical --- Device, Nanoarray Analytical --- Devices, Microarray Analytical --- Devices, Nanoarray Analytical --- Microarray Analytical Device --- Microarray Microchip --- Microchip, Microarray --- Microchips, Microarray --- Nanoarray Analytical Device --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Diagnostic immunohistology --- Culture Technique, Tissue --- Culture Techniques, Tissue --- Tissue Culture Technique --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- methods --- Cultures and culture media --- Cell Array Analysis --- Cell Microarray Analysis --- Tissue Microarray Analysis --- Analyses, Cell Array --- Analyses, Cell Microarray --- Analyses, Tissue Array --- Analyses, Tissue Microarray --- Analysis, Cell Array --- Analysis, Cell Microarray --- Analysis, Tissue Array --- Analysis, Tissue Microarray --- Array Analyses, Cell --- Array Analyses, Tissue --- Array Analysis, Cell --- Array Analysis, Tissue --- Cell Array Analyses --- Cell Microarray Analyses --- Microarray Analyses, Cell --- Microarray Analyses, Tissue --- Microarray Analysis, Cell --- Microarray Analysis, Tissue --- Tissue Array Analyses --- Tissue Microarray Analyses --- Embedding, Tissue --- Medicine. --- Pathology. --- Molecular Medicine. --- Disease (Pathology) --- Medical sciences --- Diseases --- Medicine --- Medicine, Preventive --- Health Workforce