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"Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material. Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Offers project lab formats for students that closely simulate original research projects. Provides instructional guidance for students to design their own experiments . Includes advanced analytical techniques. Contains adaptable modular exercises that allow for the study proteins other than FNR, LuxG and LDH. Includes access to a website with additional resources for instructors"--Page 4 of cover.
Enzymes --- Publication Formats --- Enzymes and Coenzymes --- Chemicals and Drugs --- Publication Characteristics --- Laboratory Manuals --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Analysis --- Biocatalysts --- Ferments --- Soluble ferments --- Catalysts --- Proteins --- Enzymology
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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cytoskeletal structure, including such topics as rotational movement of formins studied by fluorescence polarization microscopy, in vitro assembly assays for bacterial actin filaments, and modulators of microtubule plus end growth.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers cytoskeletal structureContains chapters with such topics as rec
Contractile Proteins --- Cytoplasmic Structures --- Proteins --- Cytoplasm --- Amino Acids, Peptides, and Proteins --- Intracellular Space --- Cellular Structures --- Chemicals and Drugs --- Cells --- Anatomy --- Microtubules --- Muscle Proteins --- Cytoskeleton --- Human Anatomy & Physiology --- Health & Biological Sciences --- Neuroscience --- Clinical enzymology. --- Diagnostic enzymology --- Clinical chemistry --- Enzymology
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Xylanolytic Enzymes describes the enzyme structure and its interaction with plant cell walls, the properties and production of different enzymes and their application, and the knowledge gathered on the hydrolysis mechanism of hemicellulose. The knowledge gathered about the hydrolysis mechanism of the hemicelluloses, especially xylans, has greatly promoted the rapid application of these enzymes in new areas. Recently there has been much industrial interest in xylan and its hydrolytic enzymatic complex, as a supplement and for the manufacturing of food, drinks, textiles, pulps a
Enzyme. --- Thermophilic fungi -- Biotechnology. --- Xylanases. --- Xylans. --- Xylanases --- Metabolic Phenomena --- Polysaccharides --- Enzymes and Coenzymes --- Glycoside Hydrolases --- Carbohydrates --- Chemicals and Drugs --- Hydrolases --- Phenomena and Processes --- Xylans --- Enzymes --- Xylosidases --- Metabolism --- Chemistry --- Human Anatomy & Physiology --- Physical Sciences & Mathematics --- Health & Biological Sciences --- Animal Biochemistry --- Organic Chemistry --- Industrial applications --- Hemicellulose. --- Wood-pulp --- Hydrolases. --- Microbiology. --- Industrial applications. --- Industrial enzymology --- Hydrolytic enzymes --- Cellulose --- Bacteriology --- Enzymes.
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There are many steps on the road from discovery of an anticancer drug to securing its final approval by the Food and Drug Administration. In this thoroughly updated and expanded second edition of the Handbook of Anticancer Pharmacokinetics and Pharmacodynamics, leading investigators synthesize an invaluable overview of the experimental and clinical processes of anticancer drug development, creating a single indispensable reference that covers all the steps from the identification of cancer-specific molecular targets to screening techniques and the development and validation of bioanalytical methods to clinical trial design and all phases of clinical trials. The authors have included new material on phase 0 trials in oncology, organ dysfunction trials, drug formulations and their impact on anticancer drug PK/PD including strategies to improve drug delivery, pharmacogenomics and cancer therapy, high throughput platforms in drug metabolism and transport pharmacogenetics, imaging in drug development and nanotechnology in cancer. Authoritative and up-to-date, Handbook of Anticancer Pharmacokinetics and Pharmacodynamics, 2nd Edition provides in one comprehensive and highly practical volume a detailed step-by-step guide to the successful design and approval of anticancer drugs. Road map to anticancer drug development from discovery to NDA submission Discussion of molecular targets and preclinical screening Development and validation of bioanalytical methods Chapters on clinical trial design and phase 0, I, II, III clinical trials Pharmacokinetics, pharmacodynamics, pharmacogenomics, and pharmacogenetics of anticancer agents Review of the drug development process from both laboratory and clinical perspectives New technological advances in imaging, high throughput platforms, and nanotechnology in anticancer drug development.
Antineoplastic Agents -- pharmacokinetics. --- Antineoplastic Agents -- pharmacology. --- Antineoplastic agents -- Research -- Handbooks, manuals, etc. --- Antineoplastic agents. --- Antineoplastic agents --- Therapeutic Uses --- Pharmacologic Actions --- Chemical Actions and Uses --- Chemicals and Drugs --- Antineoplastic Agents --- Health & Biological Sciences --- Pharmacy, Therapeutics, & Pharmacology --- Research --- Anticancer agents --- Antineoplastic drugs --- Antineoplastics --- Antitumor agents --- Antitumor drugs --- Cytotoxic drugs --- Inhibitors, Neoplasm --- Neoplasm inhibitors --- Pharmacy. --- Chemistry --- Medicine --- Drugs --- Materia medica --- Pharmacology --- Cancer --- Chemotherapy
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Written by leading, internationally-known scholars in itch research and treatment, this volume presents the most current update on all aspects of itch research and the clinical treatment of itch that accompanies many dermatological conditions including psoriasis, neuropathic itch, cutaneous t-cell lymphomas, and systemic diseases such as kidney and liver disease and cancer. The book is designed for basic researchers, clinicians who study and treat itch, pharmaceutical companies interested in developing itch treatments, and anyone else interested in learning about itch--Provided by publisher.
Skin Manifestations --- Skin Diseases --- Dermatologic Agents --- Signs and Symptoms --- Skin and Connective Tissue Diseases --- Therapeutic Uses --- Pathological Conditions, Signs and Symptoms --- Pharmacologic Actions --- Diseases --- Chemical Actions and Uses --- Chemicals and Drugs --- Pruritus --- Antipruritics --- Medicine --- Health & Biological Sciences --- Dermatology --- Itching --- Skin --- Treatment. --- Diseases.
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The release of pheromones is one of various forms of nonverbal communication many animals use to transmit messages to other members of the same species. Their complex molecular structure allows these chemical messages to contain a great deal of often very specific information. This book provides an overview of recent findings in the field of pheromones and chemical signals in vertebrate and invertebrate species. It includes chapters on how chemical signals work on different species and covers non-mammalian species and mammalian pheremones in general and in particular species.
Molecular neurobiology --- Neurocommunication --- Brain chemistry --- Biological Factors --- Biology --- Behavior, Animal --- Neurosciences --- Chemicals and Drugs --- Biological Science Disciplines --- Behavior --- Natural Science Disciplines --- Behavior and Behavior Mechanisms --- Psychiatry and Psychology --- Disciplines and Occupations --- Neurobiology --- Animal Communication --- Pheromones --- Human Anatomy & Physiology --- Health & Biological Sciences --- Neuroscience --- Molecular neurobiology. --- Neurocommunication. --- Brain chemistry.
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Diabetes Mellitus, a syndrome of disordered metabolism, characterised by abnormal elevation in blood glucose level, has become a life-threatening condition for many people. Current means of therapy for Diabetes Mellitus do not mimic the normal physiological pattern of insulin release. Oral delivery is the preferred route of administration due to its non-invasive nature. Oral delivery of insulin presents an overview of Diabetes Mellitus, and discusses the strategies and techniques adopted for oral delivery of insulin. This title begins with an introductory chapter on symptoms, complications and
Drug Therapy --- Drug Administration Routes --- Proinsulin --- Pancreatic Hormones --- Therapeutics --- Peptide Hormones --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Hormones --- Peptides --- Amino Acids, Peptides, and Proteins --- Hormones, Hormone Substitutes, and Hormone Antagonists --- Chemicals and Drugs --- Insulin --- Drug Delivery Systems --- Administration, Oral --- Medicine --- Health & Biological Sciences --- Clinical Endocrinology --- Diabetes --- Therapeutic use --- Administration. --- Oral therapy. --- Oral medication
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Interleukins in Cancer Biology responds to the growing need for credible and up-to-date information about the impact of interleukins on occurrence, development and progression of cancer. It provides reliable information about all known interleukins (38), describes recent discoveries in the field, and moreover, suggests further directions of research on the most promising aspects of this topic. The structure and presentation of the work is very understandable and clear with attention to detail maintained throughout. There are multiple illustrations throughout to help in comprehending and remem
Cancer. --- Interleukins. --- Neoplasms. --- Cancer --- Interleukins --- Cytokines --- Diseases --- Intercellular Signaling Peptides and Proteins --- Biological Factors --- Proteins --- Peptides --- Chemicals and Drugs --- Amino Acids, Peptides, and Proteins --- Neoplasms --- Biology --- Medicine --- Health & Biological Sciences --- Oncology --- Microbiology & Immunology --- Immunological aspects --- Cancers --- Carcinoma --- Malignancy (Cancer) --- Malignant tumors --- Tumors --- Growth factors --- Lymphocytes
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Advances in processing technologies and our understanding of materials and their properties have made it possible for scientists and engineers to develop more sophisticated biomaterials with more targeted functionality. This important book provides a concise summary of the latest developments in key types of biomaterials.The book begins with an overview of the use of biomaterials in contemporary healthcare and the process of developing novel biomaterials; the key issues and challenges associated with the design of complex implantable systems are also highlighted. The book then reviews
Biomedical engineering. --- Healthcare. --- Regenerative medicine. --- Biomedical and Dental Materials --- Manufactured Materials --- Chemicals and Drugs --- Specialty Uses of Chemicals --- Technology, Industry, and Agriculture --- Chemical Actions and Uses --- Technology, Industry, Agriculture --- Biocompatible Materials --- Health & Biological Sciences --- Biomedical Engineering --- Biomedical materials. --- Biocompatible materials --- Biomaterials --- Medical materials --- Medicine --- Biomedical engineering --- Materials --- Biocompatibility --- Prosthesis --- Bioartificial materials --- Hemocompatible materials --- Biomaterials (Biomedical materials)
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Polyphenols play key roles in the growth, regulation and structure of plants and vary widely within different plants. Stress, growth conditions and plant species modify polyphenol structure and content. Techniques to isolate as well as characterize polyphenols are reviewed. As some have adverse effects in mammals their toxicology will be carefully reviewed. Plant scientists and dietary supplement producers will be particularly interested in assessing polyphenol content and factors affecting their composition, to select sources and regulate environmental conditions affecting yield fo
Phytochemicals. --- Plant bioactive compounds. --- Plant polyphenols. --- Plants -- Analysis. --- Plant polyphenols --- Pharmaceutical Preparations --- Plant Preparations --- Natural Science Disciplines --- Eukaryota --- Biological Products --- Chemicals and Drugs --- Disciplines and Occupations --- Organisms --- Complex Mixtures --- Plant Extracts --- Plants --- Chemistry --- Botany --- Earth & Environmental Sciences --- Plant Physiology --- Plant polymers --- Polyphenols
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