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Biological Specimen Preparation for Transmission Electron Microscopy.
Authors: ---
ISBN: 0691630127 0691600155 1400865026 0691009007 9781400865024 Year: 2014 Publisher: Princeton Princeton University Press

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Abstract

This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen.The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements.All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs.Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, EditorOriginally published in 1999.The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Keywords

Mounting of microscope specimens. --- Transmission electron microscopy --- Electron microscopy --- Microscope specimens, Mounting of --- Microtechnique --- Technique. --- Acetonitrile (data page). --- Acrylic resin. --- Aldehyde. --- Animal testing. --- Antibody. --- Antigen-antibody interaction. --- Aqueous solution. --- Araldite. --- Autolysis (biology). --- Biochemist. --- Biological activity. --- Biological specimen. --- Biologist. --- Biology. --- Biopsy. --- Carbohydrazide. --- Catalysis. --- Cathode ray. --- Cell (biology). --- Cell culture. --- Cell membrane. --- Chemical formula. --- Chemical polarity. --- Chemistry. --- Chloroplast. --- Cryogenics. --- Curing (chemistry). --- Cyanide. --- Cytochemistry. --- Cytochrome c oxidase. --- Cytoplasm. --- Denaturation (biochemistry). --- Division (military). --- Electromagnetic radiation. --- Electron energy loss spectroscopy. --- Electron microscope. --- Electron tomography. --- Embedding. --- Enzyme catalysis. --- Epoxy. --- Erythrocytes. --- Ethanol. --- Ethylene glycol (data page). --- Ethylene glycol dimethacrylate. --- Ethylene glycol. --- Extraction (chemistry). --- Fixation (histology). --- Fixative (drawing). --- Formaldehyde. --- Formic acid (data page). --- Glutaraldehyde. --- Laboratory. --- Lipid. --- Macrophage (ecology). --- Micrograph. --- Microscope slide. --- Microscopy Society of America. --- Microscopy. --- Mixture. --- Moiety (chemistry). --- Mold. --- Molecular mass. --- Molecular sieve. --- Myoepithelial cell. --- Nuclear warfare. --- Nuclear weapon. --- Nuclear weapons delivery. --- Nucleic acid. --- Organic acid anhydride. --- Organic peroxide. --- Osmium tetroxide. --- Perfusion. --- Phosphate. --- Polymer. --- Polymerization. --- Polypropylene glycol. --- Propylene oxide. --- Protein structure. --- Protein. --- Radical (chemistry). --- Recrystallization (chemistry). --- Recrystallization (metallurgy). --- Resin. --- Ribosome. --- Room temperature. --- Scanning electron microscope. --- Solution. --- Solvent. --- Staining. --- Sterilization (microbiology). --- Tetrahydrofuran (data page). --- Tissue culture. --- Transmission electron microscopy. --- Triethylene glycol. --- Ultrastructure. --- Ultraviolet germicidal irradiation. --- Uranyl acetate. --- Vial. --- Viscosity. --- X-ray photoelectron spectroscopy.

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