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The seed plays a fundamental role in plant reproduction as well as a key source of energy, nutrients and raw materials for developing and sustaining humanity. With an expanding and generally more affluent world population projected to reach nine billion by mid-century, coupled to diminishing availability of inputs, agriculture is facing increasing challenges to ensure sufficient grain production. A deeper understanding of seed development, evolution and physiology will undoubtedly provide a fundamental basis to improve plant breeding practices and ultimately crop yields. Recent advances in genetic, biochemical, molecular and physiological research, mostly brought about by the deployment of novel high-throughput and high-sensitivity technologies, have begun to uncover and connect the molecular networks that control and integrate different aspects of seed development and help determine the economic value of grain crops with unprecedented details. The objective of this e-book is to provide a compilation of original research articles, reviews, hypotheses and perspectives that have recently been published in Frontiers in Plant Science, Plant Evolution and Development as part of the Research Topic entitled "Advances in Seed Biology". Editing this Research Topic has been an extremely interesting, educational and rewarding experience, and we sincerely thank all authors who contributed their expertise and in-depth knowledge of the different topics discussed. We hope that the information presented here will help to establish the state of the art of this field and will convey how exciting and important studying seeds is and hopefully will stimulate a new crop of scientists devoted to investigating the biology of seeds.

Seeds --- Plant molecular biology. --- Microbiology. --- Physiology. --- Development. --- Seed development --- Plants --- Plant physiology --- Molecular phytobiology --- Phytobiology, Molecular --- Botany --- Molecular biology --- Development --- Cell Cycle --- dormancy --- Germination --- Seed Epigenetics --- seed development --- embryo --- Endosperm --- seed metabolites --- seed coat --- Storage proteins

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The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein, and enzymatic activities. Given that the ability to uncover mitochondria’s unique features is underpinned by current methodology, this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Mitochondria: Methods and Protocols serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mitochondrial research as it links to so many important aspects of plant cell biology.

Botany. --- Plant Sciences. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Plants --- Floristic botany

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The ability to produce vast amounts of DNA sequence data has enabled the discovery of molecular markers in model organisms, crops, as well as orphan species making genotyping the rate limiting factor, and this volume focuses on the different markers available and the low to high throughput genotyping of these markers. Given the diverse nature of some of these systems, an overview is provided on the identification of markers from sequence data, as well as data analysis with example applications once the genotyping data has been generated.  Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Plant Genotyping: Methods and Protocols is aimed at plant molecular biologists, geneticists, plant breeders and ecologists who have a target question and need to know the most suitable markers and genotyping system to use.

Botany. --- Plant genetics. --- Plant Sciences. --- Plant Genetics and Genomics. --- Plants --- Genetics --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Floristic botany

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This detailed volume addresses recent developments in phosphoproteomic techniques with a particular focus on the plant system. Over the recent decades, proteomic methods were refined to study the significance and dynamics of protein phosphorylation in various biological contexts. However, working with plant tissue imposes particular challenges to the biologist which are attributed to the rigid cell wall making protein extraction more difficult, the skewed protein abundance with Rubisco as a highly abundant protein, and a large central vacuole leading to low protein yield and increased degradative enzyme activity. The methodologies in this book seek to move beyond these issues.  Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Phosphoproteomics: Methods and Protocols serves as an ideal reference for researchers investigating this vital area of plant science.

Botany. --- Proteomics. --- Plant Sciences. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Plants --- Molecular biology --- Proteins --- Floristic botany --- Photophosphorylation

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With one volume each year, this series keeps scientists and advanced students informed of the latest developments and results in all areas of the plant sciences. The present volume includes reviews on physiology, ecology and vegetation science.

Life Sciences. --- Plant Sciences. --- Life sciences. --- Botany. --- Sciences de la vie --- Botanique --- Botany --- Earth & Environmental Sciences --- Botany - General --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Plant science. --- Biology --- Natural history --- Plants --- Floristic botany