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This second edition provides updated and new chapters on T-Cell trafficking. In addition to detailed experimental procedures, the interested reader will find informative introductory chapters on the relevance of T-Cell trafficking in thymic population and maturation, traffic through secondary lymphoid organs during ‘physiological’ resolving inflammation and during immune responses, as well as T-Cell trafficking in chronic inflammatory diseases. Importantly, chapters cover methods from in silico modeling of cellular interactions, in vitro adhesion assays, through ex vivo functional assays to integrated intravital modeling of T-Cell trafficking through organs. Written in the highly successful Methods in Molecular Biology series format, each methods chapter includes a short introduction to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, T-Cell Trafficking: Methods and Protocols, Second Edition aims to be an essential point of reference for those new to the field of T-Cell trafficking, or to those looking to expand their technical capabilities.
Immunology. --- Immunobiology --- Life sciences --- Serology --- Cèl·lules T --- Motilitat cel·lular --- Moviment cel·lular --- Fisiologia cel·lular --- Cèl·lules timodependents --- Limfòcits T --- Limfòcits timodependents --- Timòcits --- Limfòcits
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This volume describes a variety of protocols that will allow the readers to study different aspects of transcriptional and posttranscriptional gene expression regulation in eukaryotic cells. Chapters focus on the latest use of CRISPRi and RNAi technologies for studying various aspects of transcriptional and posttranscriptional regulation and tools to navigate protocols on key bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Eukaryotic Transcription and Post-Transcription Gene Expression Regulation aims to ensure successful results in the further study of this vital field.
Human genetics. --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Regulació genètica --- Cèl·lules eucariotes --- Expressió gènica --- Transcripció genètica --- Manuals de laboratori --- Tècniques de laboratori --- Codi genètic --- Factors de transcripció --- Expressió dels gens --- Expressió genètica --- Expressivitat genètica --- Expressivitat gènica --- Ressenya genètica --- Vectors genètics --- Protists --- Cèl·lules --- Cèl·lules animals --- Biosíntesi --- Genètica molecular --- Inducció enzimàtica
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pilgrims [people] --- pilgrimages --- Art --- Crabeels, Cel --- Cole, Stijn --- Vansteenkiste, Jonas --- Clinckx, Christine --- Delrue, Ronny --- Nicolodi, Renato --- 378.4 <493 KUL> --- 378.4 <493 KUL> Universiteiten: Katholieke Universiteit Leuven--KUL --- Universiteiten: Katholieke Universiteit Leuven--KUL --- 7.047 --- Iconografie ; landschappen, zeegezichten, panorama's, land-art --- Thema's in de kunst ; El Camino de Santiago ; onderweg zijn --- Clinkx, Christine
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