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This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhanced hands-on insights into this evolving field. .
Immunology. --- Proteomics. --- Molecular biology --- Proteins --- Immunobiology --- Life sciences --- Serology --- Immunologia --- Proteòmica --- Manuals de laboratori --- Biologia molecular --- Proteïnes --- Biologia --- Complements (Immunitat) --- Immunofarmacologia --- Immunologia clínica --- Immunologia de la trasplantació --- Neuroimmunologia --- Resposta immunitària --- Sistema immunitari --- Tècniques de laboratori
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In this monograph, the author -Head researcher at the Institute of Cell Biophysics of Russian Academy of Sciences- discusses the results of his own long-term studies of mitochondria as well as alternative points of view and experiments of other important researchers in the field. The monograph contains the main aspects of mitochondrial research by a number of physical methods: fluorescence spectroscopy, UV-vis spectrophotometry, IR spectroscopy, light-scattering, optical microscopy, fluorescence microscopy, colorimetry, photobleaching, polarography, among others. The monograph is very useful for researchers and graduate students specializing in mitochondrial biophysics, biochemistry, molecular biology and cytology. The book was published in Russian in 2019 by Photon Publishers (Pushchino, Moscow region).
Molecular biology. --- Biophysics. --- Biological physics. --- Cell biology. --- Cell physiology. --- Pharmacology. --- Molecular Medicine. --- Biological and Medical Physics, Biophysics. --- Cell Biology. --- Cell Physiology. --- Pharmacology/Toxicology. --- Drug effects --- Medical pharmacology --- Medical sciences --- Chemicals --- Chemotherapy --- Drugs --- Pharmacy --- Cell function --- Cytology --- Physiology --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biological physics --- Physics --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Physiological effect --- Mitocondris --- Biofísica --- Biologia física --- Física biològica --- Biologia --- Física --- Absorció (Fisiologia) --- Biologia molecular --- Biònica --- Biomagnetisme --- Biomecànica --- Enginyeria biomèdica --- Física mèdica --- Interfícies biològiques --- Reologia (Biologia) --- Condriosomes --- Grànul d'Altmann --- Mitocondri --- Mitocòndria --- Protoplasma --- ADN mitocondrial
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This second edition volume expands on the previous edition with new insights into the molecular mechanisms involved in allergy through the use of various protocols that cover disciplines like allergy, immunology, cell biology, and histology. The chapters in this book cover topics such as characterization of allergens using western blotting and mass spectrometry, collecting and sampling airborne allergens, IgG antibodies, identification and characterization of allergen specific T cells, and the role of antigen presenting cells in T cell proliferation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Allergy: Methods and Protocols, Second Edition is a valuable resource for researchers interested in beginning or continuing their research in the study of allergies.
Immunology. --- Allergy. --- Allergology. --- Allergic diseases --- Allergies --- Hypersensitivity --- Hypersensitivity, Immediate --- Immediate allergy --- Immediate hypersensitivity --- Immunologic diseases --- Immunoglobulin E --- Immunobiology --- Life sciences --- Serology --- Al·lèrgia --- Immunologia --- Citologia --- Histologia --- Al·lèrgens --- Transformació genètica --- Manuals de laboratori --- Anatomia microscòpica --- Histologia humana --- Anatomia --- Cèl·lules --- Histologia veterinària --- Histopatologia --- Histoquímica --- Teixits (Histologia) --- Ultraestructura (Biologia) --- Tècniques de laboratori --- Transferència gènica --- Àcids nucleics --- Antígens --- Biologia cel·lular --- Biologia --- Citogenètica --- Citoquímica --- Migració cel·lular --- Cèl·lules animals --- Complements (Immunitat) --- Immunofarmacologia --- Immunologia clínica --- Immunologia de la trasplantació --- Neuroimmunologia --- Resposta immunitària --- Sistema immunitari --- Al·lèrgies --- Processos al·lèrgics --- Al·lèrgia alimentària --- Al·lèrgia als medicaments --- Al·lèrgia en els infants --- Dermatitis atòpica --- Dermatitis de contacte --- Fotosensibilització (Biologia) --- Urticària --- Histamina
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This book will be of immense helpful to the students of plant biotechnology, Agricultural sciences, Microbiology of both undergraduate and postgraduate levels in universities, colleges, and Research institutes. Besides the book will be quite supportive researchers who work in the field of plant biotechnology and agricultural sciences. In this book, the main focus will be on advanced genome editing approaches for the production of GM crops besides their socioeconomic, ethical and risk-biosafety assessments. Nanotechnology is the new emerging and fascinating field of science finds its application in almost all the major research areas and its uses in agriculture and food sectors are incipient.The books seems to be first in summarizing the two way interactive approach in the field of plant biotechnology and setting of a new arena in shaping the new bio techniques towards the sustainable cause.
Agriculture. --- Metabolism. --- Plant biochemistry. --- Plant physiology. --- Metabolomics. --- Plant Biochemistry. --- Plant Physiology. --- Botany --- Plants --- Physiology --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Phytochemicals --- Plant biochemical genetics --- Anabolism --- Catabolism --- Metabolism, Primary --- Primary metabolism --- Farming --- Husbandry --- Industrial arts --- Life sciences --- Food supply --- Land use, Rural --- Sustainable agriculture. --- Low-input agriculture --- Low-input sustainable agriculture --- Lower input agriculture --- Resource-efficient agriculture --- Sustainable farming --- Agriculture --- Alternative agriculture --- Agricultura sostenible --- Biologia molecular vegetal --- Biologia molecular de les plantes --- Biologia molecular --- Genètica molecular vegetal --- Fisiologia vegetal --- Fitoquímica --- Agricultura alternativa --- Agricultura integrada --- Agricultura viable --- Conreus integrats --- Producció agrícola integrada --- Producció integrada (Agricultura) --- Agricultura --- Silvicultura sostenible --- Agricultura biològica --- Agricultura de conservació --- Desenvolupament sostenible --- Ecologia agrícola --- Sistemes agrícoles
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This book focuses on recent advances regarding clinical conditions and ailments whose mechanisms remain unclear, limiting our ability to treat them. The respective chapters address a range of multidisciplinary topics related to timely or emergent research areas, such as osteoporosis in postmenopausal women, the optimal surgical procedures for vertebral compression fractures, novel rehabilitative approaches in pelvic muscle training in case of urinary incontinence, and a rational approach to balneotherapy not only for the skin but also other organ systems disorders. Alterations in the endocrine function during strenuous physical activity at high altitude – a multi-stressor environment comprising hypobaric hypoxia, exercise, and nutritional changes – are presented as well. Other articles provide evidence-based insights into the diagnosis, mechanisms, and clinical course of respiratory infections in children such as bronchiolitis, pneumonia, and influenza. Finally, the pros and cons of e-health are discussed; a rapidly growing area based on the use of information and communication technology to streamline the flow of health information and patient-healthcare provider connections. The content is a well-structured blend of research and practical aspects, as well as updates on cutting-edge developments. All these essential topics are presented in a format suitable for medical professionals engaged in day-to-day patient care and therapy, as well as researchers, academics, and physiotherapists.
Molecular biology. --- Rehabilitation medicine. --- Human physiology. --- Molecular Medicine. --- Rehabilitation Medicine. --- Human Physiology. --- Human biology --- Medical sciences --- Physiology --- Human body --- Medicine, Rehabilitation --- Rehabilitation medicine --- Rehabilitation --- Medicine, Physical --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Medical innovations. --- Innovations, Medical --- Medicine --- Medical technology --- Technological innovations --- Innovations --- Biologia molecular --- Biofísica molecular --- Bioquímica molecular --- Biofísica --- Bioquímica --- Histoquímica --- Biologia molecular vegetal --- Codi genètic --- Diagnòstic molecular --- Endocrinologia molecular --- Evolució molecular --- Farmacologia molecular --- Genètica molecular --- Glicòmica --- Metabolòmica --- Microbiologia molecular --- Neurobiologia molecular --- Patologia molecular --- Proteòmica --- Reconeixement molecular --- Biomolècules
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As one of the largest families within the Caridea, the Alpheidae have attracted much attention for its species richness, especially on coral reefs. The Alpheidae are one of the most abundant decapods in tropical and subtropical areas, with 48 genera and more than 700 known species. The Alpheidae present a particular challenge in terms of both taxonomy and systematics as they are difficult to identify (some species vary in their growth and there are often large differences between the sexes). Traditional, morphology-based research on the Alpheidae still plays an important role in identifying species, compared with new methods, such as the short gene sequences. Based on more than 2000 specimens collected from the China Seas, this book describes and clearly illustrates 146 species belonging to 16 genera of the Alpheidae. It also presents the key features of every genus and every species within every genus, to enable readers to easily identify the alphid shrimps of the China Seas.
Oceanography. --- Oceanography, Physical --- Oceanology --- Physical oceanography --- Thalassography --- Earth sciences --- Marine sciences --- Ocean --- Animal systematics. --- Animal taxonomy. --- Animal anatomy. --- Marine sciences. --- Freshwater. --- Animal ecology. --- Biodiversity. --- Invertebrates. --- Animal Systematics/Taxonomy/Biogeography. --- Animal Anatomy / Morphology / Histology. --- Marine & Freshwater Sciences. --- Animal Ecology. --- Invertebrata --- Animals --- Biological diversification --- Biological diversity --- Biotic diversity --- Diversification, Biological --- Diversity, Biological --- Biology --- Biocomplexity --- Ecological heterogeneity --- Numbers of species --- Zoology --- Ecology --- Fresh waters --- Freshwater --- Freshwaters --- Inland water --- Inland waters --- Water --- Ocean sciences --- Aquatic sciences --- Animal anatomy --- Physiology --- Animal classification --- Animal systematics --- Animal taxonomy --- Classification --- Systematic zoology --- Systematics (Zoology) --- Taxonomy, Animal --- Zoological classification --- Zoological systematics --- Zoological taxonomy --- Anatomy --- Taxonomia (Biologia) --- Xina --- Biologia sistemàtica --- Biosistemàtica --- Biotaxonomia --- Classificació (Biologia) --- Classificació dels organismes vius --- Sistemàtica (Biologia) --- Taxonomia --- Classificació --- Cladística --- Espècies (Biologia) --- Homologia (Biologia) --- Quimiotaxonomia --- Taxonomia botànica --- Taxonomia numèrica --- Taxonomia zoològica --- Cathay --- China --- República de la Xina (1912-1949) --- República Popular de la Xina --- República Popular Xina --- Zhonghua Renmin Gongheguo --- Xina (República : 1912-1949) --- Àsia oriental --- Canton (Xina) --- Guangdong (Xina) --- Hainan (Xina : Sheng) --- Henan (Xina : Sheng) --- Hong Kong (Xina) --- Macau (Xina : Regió administrativa especial) --- Iang-Tsé (Xina : Curs d'aigua) --- Manxúria (Xina : Regió) --- Mongòlia Interior (Xina : Zizhiqu) --- Pequín (Xina) --- Sichuan (Xina : Sheng) --- Xinjiang (Xina : Regió) --- Xangai (Xina) --- Yunnan (Xina : Sheng) --- Brahmaputra (Àsia : Curs d'aigua) --- Tibet (Xina)
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This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful Methods in Molecular Biology series, chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Protein Production and Purification: Methods and Protocols serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study.
Biochemistry. --- Protein Science. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Proteïnes --- Bioquímica --- Manuals de laboratori --- Tècniques de laboratori --- Química biològica --- Química fisiològica --- Biologia --- Medicina --- Química --- Absorció (Fisiologia) --- Anàlisi enzimàtica --- Biodegradació --- Biodisponibilitat --- Bioelectroquímica --- Bioenergètica --- Biogeoquímica --- Biologia molecular --- Bioquímica analítica --- Bioquímica clínica --- Bioquímica física --- Bioquímica quàntica --- Biosíntesi --- Centres actius (Bioquímica) --- Citoquímica --- Composició del cos humà --- Cromatografia --- Digestió --- Enginyeria bioquímica --- Enzimologia --- Equilibri àcid-base --- Fitoquímica --- Fixació de proteïnes --- Genètica bioquímica --- Immunoquímica --- Interfícies biològiques --- Lípids --- Lligands (Bioquímica) --- Marcadors bioquímics --- Metabolisme --- Minerals en l'organisme --- Neuroquímica --- Oxidació fisiològica --- Pigments (Biologia) --- Química biorgànica --- Química clínica --- Química microbiològica --- Quimiotaxi --- Quimiotaxonomia --- Toxicologia bioquímica --- Xenobiòtics --- Histoquímica --- Biomolècules --- Albúmines --- Apoproteïnes --- Conformació de proteïnes --- Enzims --- Factors de transcripció --- Glicoproteïnes --- Lipoproteïnes --- Nucleoproteïnes --- Pèptids --- Prions --- Protamines --- Proteïnes citosquelètiques --- Proteïnes de membrana --- Proteïnes de la sang --- Proteïnes recombinants --- Proteïnes supressores de tumors --- Receptors cel·lulars --- Ubiqüitina --- Xaperones moleculars --- Proteòmica
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This book discusses novel concepts and discoveries concerning the regulation of innate immunity by autophagy and autophagy-related proteins. In the past decade, there have been major advances in our understanding of the molecular mechanisms of autophagy and its physiological functions. This book highlights emerging studies on the underlying mechanisms of autophagy regulation of innate immunity, including inflammation, antiviral immunity and anti-bacterial responses and the signaling pathways that prompt or inhibit the initiation and progression of related diseases. It also offers new ideas and strategies for future drugs based on manipulating autophagy, especially selective autophagy mediated by cargo receptors. Providing a comprehensive overview of the autophagy regulation of innate immunity, it is a valuable resource for graduate students and researchers in the fields of immunology, cell biology and translational medicine.
Immunology. --- Cell biology. --- Molecular biology. --- Cell Biology. --- Molecular Medicine. --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Immunobiology --- Life sciences --- Serology --- Immunologia --- Biologia --- Complements (Immunitat) --- Immunofarmacologia --- Immunologia clínica --- Immunologia de la trasplantació --- Neuroimmunologia --- Resposta immunitària --- Sistema immunitari
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This book provides thorough coverage of high-throughput OMICs technologies for the monitoring of stem cells and regenerative medicine. Specific topics covered include the genomics, proteomics, and metabolomics aspects of regenerative medicine, metabolic profiling of mesenchymal stem cells, genome profiling of mesenchymal stem cells, OMICs monitoring of stem cell-derived exosomes, stem cell proteomics, lipidomics, OMICs profiling of cancer (stem) cells, and finally ethical considerations of OMICs-based investigations. Chapters are authored by world-renowned scientists who have valuable expertise in the field of OMICs and regenerative medicine. Genomics, Proteomics, and Metabolomics: Stem Cells Monitoring in Regenerative Medicine, part of Springer’s Stem Cell Biology and Regenerative Medicine series, is essential reading for researchers, clinicians, biologists, biochemists, and pharmaceutical experts conducting research in the fields of stem cell biology, molecular aspects of stem cell research, tissue engineering, regenerative medicine, cellular therapy, OMICs, bioinformatics, and ethics.
Stem cells. --- Regenerative medicine. --- Tissue engineering. --- Proteomics. --- Animal genetics. --- Metabolism. --- Stem Cells. --- Regenerative Medicine/Tissue Engineering. --- Animal Genetics and Genomics. --- Metabolomics. --- Anabolism --- Catabolism --- Metabolism, Primary --- Primary metabolism --- Biochemistry --- Physiology --- Genetics --- Molecular biology --- Proteins --- Biomedical engineering --- Regenerative medicine --- Tissue culture --- Medicine --- Regeneration (Biology) --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cells --- Medicina regenerativa --- Medicina --- Regeneració (Biologia) --- Enginyeria de teixits
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This book equips young immunologists and health professionals with a clear understanding of the fundamental concepts and roles of co-signal molecules and in addition presents the latest information on co-stimulation. The first part of the book is devoted to co-signal molecules and the regulation of T cells. Following an initial overview, subsequent chapters examine each co-signal molecule in turn and discuss the mechanisms by which co-signal molecules regulate the different types of T cell. The second part covers various clinical applications, including in autoimmune disease, neurological disorders, transplantation, graft-versus-host disease, and cancer immunotherapy. To date, co-stimulation blockade and co-inhibition blockade have shown beneficial effects and many additional clinical trials targeting co-signal molecules are ongoing. The mechanisms underlying these successful treatments are explained and the future therapeutic potential in the aforementioned diseases is evaluated. Co-signal Molecules in T Cell Activation will be a valuable reference guide to co-stimulation for basic and clinical researchers in the fields of both immunology and pharmaceutical science. .
Immunology. --- Cancer research. --- Neurology . --- Oncology . --- Cancer Research. --- Neurology. --- Oncology. --- Tumors --- Medicine --- Nervous system --- Neuropsychiatry --- Cancer research --- Immunobiology --- Life sciences --- Serology --- Diseases --- Cèl·lules T --- Immunologia --- Biologia --- Complements (Immunitat) --- Immunofarmacologia --- Immunologia clínica --- Immunologia de la trasplantació --- Neuroimmunologia --- Resposta immunitària --- Sistema immunitari --- Cèl·lules timodependents --- Limfòcits T --- Limfòcits timodependents --- Timòcits --- Limfòcits
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