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This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Vacunes --- ADN --- Àcid desoxiribonucleic --- DNA --- Àcids nucleics --- ADN fòssil --- ADN recombinant --- Elements genètics mòbils --- Gens --- Vacunació --- Vacunes antibacterianes --- Vacuna de la malària --- Vacunes antivíriques --- Immunitat --- Immunology. --- Medical genetics. --- Medical Genetics. --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Immunobiology --- Life sciences --- Serology --- Genetic aspects

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RNA. --- DNA damage. --- Ribonucleic acid --- Ribose nucleic acid --- Nucleic acids --- Ribose --- Damage, DNA --- Biochemical genetics --- Mutation (Biology) --- ADN --- Reparació de l'ADN --- Biomolècules --- Procariotes --- Microorganismes --- Cianobacteris --- Molècules biològiques --- Molècules --- Àcids nucleics --- Biopolímers --- Metabòlits --- Proteïnes --- Biologia molecular --- Mecanismes de reparació de l'àcid desoxiribonucleic --- Reparació de l'àcid desoxiribonucleic --- Genètica bioquímica --- Medicaments antineoplàstics --- Àcid desoxiribonucleic --- DNA --- ADN fòssil --- ADN recombinant --- Elements genètics mòbils --- Gens

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This book focuses on quinoa, providing background information on its history, summarizing recent genetic and genomic advances, and offering directions for future research. Meeting the caloric and nutritional demands of our growing population will not only require increases in overall food production, but also the development of new crops that can be grown sustainably in agricultural environments that are increasingly susceptible to degradation. Quinoa is an ancient crop native to the Andean region of South America that has recently gained international attention because its seeds are high in protein, particularly in essential amino acids. Quinoa is also highly tolerant of abiotic stresses, including drought, frost and salinity. For these reasons, quinoa has the potential to help address issues of food security – a potential that was recognized when the United Nations declared 2013 the International Year of Quinoa. However, more effort is needed to improve quinoa agronomically and to understand the mechanisms of its abiotic stress tolerance; the recent development of genetic and genomic tools, including a reference genome sequence, will now help accelerate research in these areas.

Plant genetics. --- Plant breeding. --- Agriculture. --- Plant Genetics and Genomics. --- Plant Breeding/Biotechnology. --- Farming --- Husbandry --- Industrial arts --- Life sciences --- Food supply --- Land use, Rural --- Crops --- Agriculture --- Breeding --- Plants --- Genetics --- Cariofil·làcies --- Llavors --- Genètica vegetal --- Seqüència de nucleòtids --- Anàlisi seqüencial d'àcids nucleics --- Seqüència ADN --- Seqüència de l'ADN --- Seqüències de nucleòtids --- Seqüències dels nucleòtids --- Codi genètic --- Fitogenètica --- Genètica de les plantes --- Genètica --- Genètica molecular vegetal --- Nombre de cromosomes de les plantes --- Millorament selectiu de plantes --- Botànica --- Multiplicació de les plantes --- Clavells

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This book gives a comprehensive overview of the unique roles that non-coding repetitive elements such as satellite DNAs play in different physiological and evolutionary processes. It presents the gene-regulatory aspect of satellite DNAs in different model systems including mammals, insects and plants. In addition, evolutionary aspects of activation of satellite DNAs in terms of transcription and proliferation are highlighted, revealing the role of satellite DNAs in the process of adaptation to changing environment and in the speciation process. Finally, the book discusses satellite DNA activation during pathological transformation and the mechanisms by which they affect disease progression. Namely, some satellite DNAs promote the oncogenic processes by affecting genome epigenetic regulation as well as genome integrity. Readers get a full overview of the latest research on satellite DNA.

Genetics. --- Chemistry. --- Biomaterials. --- Cell biology. --- Genetics and Genomics. --- Chemistry/Food Science, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Physical sciences --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Satellite DNA. --- Cell physiology. --- Molecular evolution. --- Biochemical evolution --- Chemical evolution --- Evolution --- Life --- Molecular biology --- Cell function --- Cytology --- Physiology --- Satellite deoxyribonucleic acid --- DNA --- Origin --- ADN --- Fisiologia cel·lular --- Evolució molecular --- Evolució bioquímica --- Evolució química --- Biologia molecular --- Evolució --- Origen de la vida --- ADN fòssil --- Fisiologia --- Motilitat cel·lular --- Àcid desoxiribonucleic --- Àcids nucleics --- ADN recombinant --- Elements genètics mòbils --- Gens

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Genetic transformation is a key technology, in which genes are transferred from one organism to another in order to improve agronomic traits and ultimately help humans. However, there is apprehension in some quarters that genetically modified crops may disturb the ecosystem. A number of non-governmental organizations continue to protest against GM crops and foods, despite the fact that many organisms are genetically modified naturally in the course of evolution. In this context, there is a need to educate the public about the importance of GM crops in terms of food and nutritional security. This book provides an overview of various crop plants where genetic transformation has been successfully implemented to improve their agronomically useful traits. It includes information on the gene(s) transferred, the method of gene transfer and the beneficial effects of these gene transfers and agronomic improvements compared to the wild plants. Further, it discusses the commercial prospects of these GM crops as well as the associated challenges. Given its scope, this book is a valuable resource for agricultural and horticultural scientists/experts wanting to explain to the public, politicians and non-governmental organizations the details of GM crops and how they can improve crops and the lives of farmers.

Plant genetics. --- Plant breeding. --- Plant biochemistry. --- Plant physiology. --- Agriculture. --- Nucleic acids. --- Plant Genetics and Genomics. --- Plant Breeding/Biotechnology. --- Plant Biochemistry. --- Plant Physiology. --- Nucleic Acid Chemistry. --- Polynucleotides --- Biomolecules --- Farming --- Husbandry --- Industrial arts --- Life sciences --- Food supply --- Land use, Rural --- Botany --- Plants --- Physiology --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Phytochemicals --- Plant biochemical genetics --- Crops --- Agriculture --- Breeding --- Genetics --- Transgenic plants. --- GE crops (Genetically engineered crops) --- GE plants (Genetically engineered plants) --- Genetically engineered crops --- Genetically engineered plants --- Genetically modified crops --- Genetically modified plants --- GM crops (Genetically modified crops) --- GM plants (Genetically modified plants) --- Novel crops --- Transgenic crops --- Plants, Cultivated --- Transgenic organisms --- Plant genetic engineering --- Transformació genètica --- Millorament selectiu de plantes --- Aliments transgènics --- Aliments genèticament modificats --- Aliments modificats genèticament --- Transgènics --- Aliments --- Biotecnologia alimentària --- Millora de plantes --- Millorament de conreus --- Millorament genètic de conreus --- Millorament genètic de les plantes --- Millorament genètic de plantes conreades --- Millorament vegetal --- Selecció artificial de les plantes --- Agricultura --- Millora de les espècies --- Hibridació vegetal --- Immunitat de les plantes --- Genètica vegetal --- Plantes transgèniques --- Transferència gènica --- Àcids nucleics