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This detailed book examines experimental approaches used to investigate the regulation and function of phosphoinositides (PtdInsP), rare eukaryotic phospholipids with a broad role in biological processes such as signal transduction, cell migration and adhesion, cell growth, subcellular organization, and membrane trafficking. The combination of complementary biochemical, mass spectrometry, and imaging methods are instrumental for the detection and quantification of PtdInsP species, as well as induced dimerization methods, affinity precipitation or co-sedimentation with liposomes, protein insertion within lipid bilayers, and enzymatic assays, or through emerging methods like native mass spectrometry and microfluidics, all of which are touched upon in this volume. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Phosphoinositides: Methods and Protocols provides detailed methodology for both specialist and novice researchers on a variety of complementary methods that have been instrumental in dissecting the regulation, dynamics, and function of PtdInsPs.

Fosfolípids --- Lípids --- Factor d'activació plaquetari --- Fosfatidilinositols --- Lecitina --- Lipids. --- Cytology. --- Lipidology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Lipides --- Lipins --- Lipoids --- Biomolecules --- Steroids

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This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments and organelles; lipid signaling, lipid-protein interactions, and imaging; and lipid databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Lipids: Methods and Protocols is a valuable guide for experienced researchers and undergraduate, graduate, and Ph.D. students. This book is also an excellent resource for novice scientists with little to no experience in lipid experiments who are interested in approaching this field experimentally. .

Botany. --- Lipids. --- Plant Science. --- Lipidology. --- Lipides --- Lipins --- Lipoids --- Biomolecules --- Steroids --- Botanical science --- Floristic botany --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Plants

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This book describes the recent advances in natural product chemistry and biodiversity in the Red Sea. All previous marine natural products isolated from different Red Sea organisms and microbes were collected in a comprehensive review. Additionally, newly discovered marine natural products and their biological activities are described.

Research & information: general --- Aspergillus falconensis --- OSMAC --- azaphilones --- X-ray diffraction --- NF-κB inhibition --- LC-HRESIMS --- Stylissa carteri --- ceramide --- cerebroside --- docking --- cytotoxic activity --- co-cultivation --- phenazine --- sponge-associated actinomycetes --- antibacterial --- antibiofilm --- DNA gyrase --- pyruvate kinase --- ergosterol derivative --- metabolic analysis --- docking studies --- seagrass --- Thalassodendron ciliatum --- Red Sea sponges --- marine actinomycetes --- Streptomyces coelicolor LY001 --- halogenated 3-phenylpropanoic acid derivatives --- diketopiperazine alkaloids --- structural determinations --- antimicrobial activities --- Red Sea --- marine natural products --- marine organisms --- biodiversity --- marine metagenomics --- bioactivity --- Sinularia polydactyla --- soft coral --- steroids --- cytotoxic --- anti-inflammatory --- neuroprotective --- androgen receptor --- Actinokineospora --- Rhodococcus --- co-culture --- metabolomics --- antimalarial --- epicotripeptin --- phragamide --- Epicoccum --- Alternaria --- antimicrobial --- n/a

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This book covers the most recent research trends and applications of Pharmaceutical Analytical Chemistry. The included topics range from the adulteration of dietary supplements, to the determination of drugs in biological samples with the aim to investigate their pharmacokinetic properties.

growth hormone --- long-acting Fc-fusion recombinant human growth hormone --- method validation --- cell-based bioassay --- reporter gene assay --- pharmacokinetics --- tissue distribution --- alnustone --- rats --- LC-MS/MS --- inflammatory bowel disease --- fixed-dose combination --- biomimetic chromatography --- thiopurine immunosuppressants --- folic acid --- doxorubicin --- hernandezine --- pharmacokinetic study --- drug–drug interaction --- gardneramine --- monoterpenoid indole alkaloid --- memantine --- rimantadine --- amantadine --- zone fluidics --- o-phthalaldehyde --- derivatization --- stopped-flow --- quality control --- anwuligan --- rat --- optode --- polyhexamethylene biguanide hydrochloride --- lactone-rhodamine B --- contact-lens detergent --- dietary supplement --- adulteration --- low-field NMR --- multivariate analysis --- steroids --- Partial Least Squares regression --- in vitro permeability --- predictive model --- ketamine --- norketamine --- high throughput bar adsorptive microextraction --- LVI-GC-MS(SIM) --- urine --- baricitinib --- UPLC-MS/MS --- irbersartan --- n/a --- drug-drug interaction

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This volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts. This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning more about the function of lipid rafts in many areas of cell biology and medicine. .

Lípids de la sang --- Lípids en la sang --- Lípids plasmàtics --- Lípids sanguinis --- Lípids sèrics --- Lípids --- Colesterol --- Lipoproteïnes de la sang --- Hiperlipèmia --- Lipids. --- Mass spectroscopy. --- Lipidology. --- Mass Spectrometry. --- Mass spectra --- Mass spectrograph --- Mass spectroscopy --- Mass spectrum analysis --- Mass (Physics) --- Nuclear spectroscopy --- Spectrum analysis --- Lipides --- Lipins --- Lipoids --- Biomolecules --- Steroids --- Espectroscòpia --- Anàlisi d'espectre --- Anàlisi dels espectres --- Anàlisi espectral --- Anàlisi espectroscòpica --- Anàlisi espectroquímica --- Espectres (Física) --- Espectroanàlisi --- Espectrografia --- Espectrometria --- Espectroquímica --- Astrofísica --- Interferometria --- Òptica --- Química --- Química analítica qualitativa --- Radiació --- Teoria del moviment ondulatori --- Diagrames de Grotrian --- Espectre ultraviolat --- Espectres atòmics --- Espectrometria de raigs alfa --- Espectrometria de raigs beta --- Espectroscòpia atòmica --- Espectroscòpia d'alta resolució --- Espectroscòpia d'electrons --- Espectroscòpia de làser --- Espectroscòpia de luminiscència --- Espectroscòpia de mobilitat d'ions --- Espectroscòpia de plasma --- Espectroscòpia molecular --- Espectroscòpia nuclear --- Espectroscòpia Raman --- Espectroscòpia de ressonància paramagnètica electrònica --- Radiació ultraviolada --- Ressonància magnètica nuclear --- Astronomia --- Espectre solar --- Espectres d'absorció --- Espectroscopis --- Llum --- Lipoides --- Bioquímica --- Esfingolípids --- Fosfolípids --- Glicolípids --- Lipoproteïnes --- Membranes lipídiques --- Terpens --- Esteroides --- Metabolisme dels lípids