TY - BOOK ID - 143483 TI - Nonradioactive analysis of biomolecules. PY - 2000 SN - 3540646019 3642572065 PB - Berlin : Springer, DB - UniCat KW - Analytical biochemistry KW - Acide nucléique KW - Nucleic acids KW - Protéine KW - proteins KW - Glycoprotéine KW - Glycoproteins KW - Biologie moléculaire KW - Molecular biology KW - Génie génétique KW - genetic engineering KW - Biomolecules KW - Analysis KW - Nucleic Acids, Nucleotides, and Nucleosides KW - Luminescence KW - Photometry KW - Amino Acids, Peptides, and Proteins KW - Molecular Probes KW - Chemistry Techniques, Analytical KW - Chemicals and Drugs KW - Light KW - Laboratory Chemicals KW - Investigative Techniques KW - Radiation, Nonionizing KW - Specialty Uses of Chemicals KW - Optical Phenomena KW - Radiation KW - Chemical Actions and Uses KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Physical Phenomena KW - Phenomena and Processes KW - Electromagnetic Phenomena KW - Fluorescence KW - Proteins KW - Luminescent Measurements KW - Nucleic Acid Probes KW - Nucleic Acids KW - Human Anatomy & Physiology KW - Health & Biological Sciences KW - Animal Biochemistry KW - Fluorescence. KW - analysis. KW - Cell biology. KW - Biochemistry. KW - Cell Biology. KW - Biochemistry, general. KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Biology KW - Chemistry KW - Medical sciences KW - Cell biology KW - Cellular biology KW - Cells KW - Composition UR - https://www.unicat.be/uniCat?func=search&query=sysid:143483 AB - New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids - are presented in the second edition of this successful laboratory manual. Highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation, as well as PCR amplification are described in detail. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques. Thus the manual will enable scientists to plan, design and conduct the appropriate procedures which fulfill their particular requirements. ER -