TY - BOOK ID - 19030218 TI - Recombinant Protein Production in Yeast : Methods and Protocols PY - 2012 SN - 1617797693 9781617797699 1617797707 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Pichia KW - Recombinant Proteins KW - Recombinant proteins KW - Saccharomyces cerevisiae KW - Yeast fungi KW - Yeasts KW - biosynthesis KW - Biotechnology KW - Fungi KW - Proteins KW - Publication Formats KW - Saccharomyces KW - Saccharomycetales KW - Publication Characteristics KW - Ascomycota KW - Eukaryota KW - Amino Acids, Peptides, and Proteins KW - Chemicals and Drugs KW - Organisms KW - Laboratory Manuals KW - Health & Biological Sciences KW - Biomedical Engineering KW - Baker Yeast KW - S cerevisiae KW - Baker's Yeast KW - Brewer's Yeast KW - Yeast, Baker's KW - Yeast, Brewer's KW - Baker Yeasts KW - Baker's Yeasts KW - Bakers Yeast KW - Brewer Yeast KW - Brewer's Yeasts KW - Brewers Yeast KW - Yeast, Baker KW - Yeast, Bakers KW - Yeast, Brewer KW - Yeast, Brewers KW - Yeasts, Baker KW - Yeasts, Baker's KW - Yeasts, Brewer's KW - Proteins, Biosynthetic KW - Proteins, Recombinant DNA KW - Biosynthetic Proteins KW - DNA Recombinant Proteins KW - DNA Proteins, Recombinant KW - Proteins, DNA Recombinant KW - Proteins, Recombinant KW - Recombinant DNA Proteins KW - Recombinant Proteins, DNA KW - Hansenula KW - Hansenulas KW - Pichias KW - Yeast KW - Genetically engineered proteins KW - Eucarya KW - Eukarya KW - Eukaryotes KW - Eukaryotas KW - Eukaryote KW - Ascomycetes KW - Cochliobolus KW - Sclerotinia KW - Ascomycete KW - Ascomycotas KW - Sclerotinias KW - Budding Yeast KW - Endomycetales KW - Endomycopsis KW - Yeast, Budding KW - Budding Yeasts KW - Endomycetale KW - Endomycopses KW - Saccharomycetale KW - Yeasts, Budding KW - Gene Products, Protein KW - Gene Proteins KW - Protein Gene Products KW - Proteins, Gene KW - Fungi, Filamentous KW - Molds KW - Filamentous Fungi KW - Filamentous Fungus KW - Fungus KW - Fungus, Filamentous KW - Mold KW - Genetic engineering KW - Saccharomyce KW - Medicine. KW - Human genetics. KW - Biomedicine. KW - Human Genetics. KW - Genetics KW - Heredity, Human KW - Human biology KW - Physical anthropology KW - Clinical sciences KW - Medical profession KW - Life sciences KW - Medical sciences KW - Pathology KW - Physicians UR - https://www.unicat.be/uniCat?func=search&query=sysid:19030218 AB - In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host. ER -