TY - BOOK ID - 26372028 TI - PCR 2 : A practical approach. AU - McPherson, M. J. AU - Hames, B. D. AU - Taylor, Doc PY - 1995 SN - 0199631964 0199634246 0199634254 019963226X 9780199634255 9780199634248 PB - Oxford : IRL Press, DB - UniCat KW - General biochemistry KW - DNA (deoxyribonucleic acid) KW - biochemie KW - PCR (polymerase chain reaction) KW - genetische informatie KW - Molecular biology KW - Analytical biochemistry KW - biologie KW - moleculaire biologie KW - polymeren KW - Macromolecules KW - Gene Amplification KW - Réaction en chaîne de la polymérase KW - Technique des traceurs KW - Tracer techniques KW - État physique KW - physical states KW - Polymerase Chain Reaction. KW - -Chain reaction, Polymerase KW - PCR (Biochemistry) KW - DNA polymerases KW - Amplification, Gene KW - Anchored Polymerase Chain Reaction KW - Inverse Polymerase Chain Reaction KW - Nested Polymerase Chain Reaction KW - Anchored PCR KW - Inverse PCR KW - Nested PCR KW - PCR, Anchored KW - PCR, Inverse KW - PCR, Nested KW - Polymerase Chain Reactions KW - Reaction, Polymerase Chain KW - Reactions, Polymerase Chain KW - Ligase Chain Reaction KW - -Molecular bases of life. Molecular biology KW - -577.2 Molecular bases of life. Molecular biology KW - Chain reaction, Polymerase KW - Réaction en chaîne de la polymérase KW - Polymerase chain reaction KW - DNA Replication KW - Polymerase Chain Reaction KW - 577.2 KW - 577.2 Molecular bases of life. Molecular biology KW - Molecular bases of life. Molecular biology KW - PCR KW - Replication, Autonomous KW - Autonomous Replication KW - Autonomous Replications KW - DNA Replications KW - Replication, DNA KW - Replications, Autonomous KW - Replications, DNA KW - DNA-Binding Proteins KW - Virus Replication KW - S Phase KW - Laboratory manuals KW - #WSCH:WBIO KW - Polymerization KW - methods KW - Laboratory manuals. KW - Polymerase chain reaction. KW - Dna replication. KW - Gene amplification. KW - DNA Replication. KW - Gene Amplification. KW - klonen KW - enzymen KW - Polymerase chain reaction - Laboratory manuals. KW - Genes UR - https://www.unicat.be/uniCat?func=search&query=sysid:26372028 AB - PCR (polymerase chain reaction) is now one of the most widely used of basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification - allowing the researcher to produce large quantities of DNA from minute amounts of starting material.PCR: A Practical Approach Volume 2 is not a revised version of PCR: A Pracical Approach, but sets out to address some of the exciting new applications fo PCR including cDNA cloning, mRNA quantitation and expression of proteins from PCR products, genomic DNA mapping and fingerprinting, and mutational analysis. The Polymerase Chain Reaction (PCR) has become one of the most widely used techniques in biomedical research, enabling the fast, inexpensive production of large quantities of DNA from minute amounts of starting material. Although the basic procedure is very simple, many variations of it have been developed for different applications, for use in such diverse areas as forensic and diagnostic medicine, genetic engineering, and the food industry. PCR: A Practical Approach provides a general introduction to PCR for researchers new to the area, followed by chapters detailing specific applications of the technique. It covers gene analysis at the level of restriction enzyme polymorphism, point mutation and sequence analysis. Methods for using different source materials - cloned DNA, genomic DNA, RNA, nucleic acid from archive material and PCR products themselves - are given. The emphasis throughout is on the practical applications of the technique and detailed experimental protocols are included at all stages, backed up by advice on avoiding potential pitfalls. This volume provides a starting point for those new to PCR and will also serve as a useful reference for more experienced researchers in all areas of biological science. ER -