TY - BOOK ID - 5297059 TI - DNA Sequencing Protocols AU - Graham, Colin A. AU - Hill, Alison J.M. PY - 2001 VL - 167 SN - 0896037169 0896037215 9786610820887 1280820888 1592591132 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Nucleotide sequence KW - Génome humain KW - Laboratory manuals KW - Séquençage KW - Manuels de laboratoire KW - DNA. KW - Electronic books. -- local. KW - Nucleotide sequence -- Laboratory manuals. KW - Sequence Analysis KW - Genetic Techniques KW - Investigative Techniques KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Sequence Analysis, DNA KW - Human Anatomy & Physiology KW - Health & Biological Sciences KW - Animal Biochemistry KW - Génome humain KW - Séquençage KW - Deoxyribonucleic acid KW - Desoxyribonucleic acid KW - Thymonucleic acid KW - TNA (Nucleic acid) KW - Deoxyribose KW - Nucleic acids KW - Genes KW - Analysis, Nucleic acid sequence KW - Analysis, Nucleotide sequence KW - Base sequence (Nucleic acids) KW - DNA sequence KW - Nucleic acid sequence analysis KW - Nucleotide sequence analysis KW - RNA sequence KW - Sequence, Nucleotide KW - Nucleotides KW - Sequence alignment (Bioinformatics) KW - Analysis KW - Cytology. KW - Cell Biology. KW - Cell biology KW - Cellular biology KW - Biology KW - Cells UR - https://www.unicat.be/uniCat?func=search&query=sysid:5297059 AB - Since the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham and a team of leading investigators and expert clinical scientists update the first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. Each method provides detailed, step-by-step instructions to ensure successful results, and includes helpful notes identifying and addressing potential problems. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will impact today's genome sequencing projects, improve the accuracy and quality of DNA sequences obtained by smaller laboratories, and help lay the foundation for molecular diagnostics. ER -