TY - BOOK ID - 5451149 TI - Nuclear Transfer Protocols : Cell Reprogramming and Transgenesis PY - 2006 VL - 348 SN - 10643745 SN - 9781588292803 1588292800 9781597451543 1597451541 1280832444 9786610832446 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Cell Nucleus KW - Cloning, Organism KW - Cell Differentiation KW - Gene Transfer Techniques. KW - Cell nuclei KW - Gene therapy. KW - Transgenic animals. KW - Thérapie génique KW - Animaux transgéniques KW - transplantation. KW - methods. KW - genetics. KW - physiology. KW - Transplantation. KW - Gene therapy KW - Transgenic animals KW - Gene Transfer Techniques KW - Transplantation KW - methods KW - genetics KW - Cell nuclei -- Transplantation. KW - Investigative Techniques KW - Surgical Procedures, Operative KW - Genetic Techniques KW - Publication Formats KW - Cell Physiological Processes KW - Biology KW - Biological Science Disciplines KW - Reproductive Techniques KW - Intracellular Space KW - Organelles KW - Cell Physiological Phenomena KW - Publication Characteristics KW - Cytoplasmic Structures KW - Natural Science Disciplines KW - Cellular Structures KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Disciplines and Occupations KW - Cells KW - Cytoplasm KW - Phenomena and Processes KW - Anatomy KW - Laboratory Manuals KW - Methods KW - Physiology KW - Genetics KW - Medicine KW - Health & Biological Sciences KW - Cytology KW - Pathology KW - Therapy, Gene KW - Nuclear transfer KW - Nuclear transplantation KW - Transplantation of cell nuclei KW - Life sciences. KW - Cell biology. KW - Life Sciences. KW - Cell Biology. KW - Cell biology KW - Cellular biology KW - Cytologists KW - Biosciences KW - Sciences, Life KW - Science KW - Animals KW - Transgenic organisms KW - Animal genetic engineering KW - Genetic engineering KW - Therapeutics KW - Reproduction KW - Cytology. KW - Cell nuclei - Transplantation KW - Cloning, Organism - methods - laboratory manuals KW - Cell Differentiation - genetics - laboratory manuals KW - Gene Transfer Techniques - laboratory Manuals UR - https://www.unicat.be/uniCat?func=search&query=sysid:5451149 AB - Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. ER -