TY - BOOK ID - 25709634 TI - DNA Microarrays : A Practical Approach PY - 2000 VL - 205 SN - 0199637768 0199637776 9780199637775 9780199637768 PB - Oxford New York Oxford University Press DB - UniCat KW - micro-elektronica KW - Molecular biology KW - DNA (deoxyribonucleic acid) KW - moleculaire biologie KW - Puces à ADN KW - Polymorphisme KW - Polymorphism KW - DNA microarrays. KW - Escherichia coli KW - Saccharomyces cerevisiae KW - ADN KW - DNA KW - ARN KW - RNA KW - gene expression KW - Puces à ADN KW - DNA. KW - RNA. KW - bio-informatica KW - klonen KW - parallel processing KW - oligonucleotides KW - microarrays KW - enzymen KW - DNA microarrays KW - Oligonucleotide Array Sequence Analysis KW - 575.3 KW - 575.3 Molecular genetics KW - Molecular genetics KW - DNA Arrays KW - DNA Chips KW - DNA Microchips KW - Gene Chips KW - Oligodeoxyribonucleotide Array Sequence Analysis KW - Oligonucleotide Microarrays KW - Sequence Analysis, Oligonucleotide Array KW - cDNA Arrays KW - DNA Microarrays KW - Oligonucleotide Arrays KW - cDNA Microarrays KW - Array, DNA KW - Array, Oligonucleotide KW - Array, cDNA KW - Arrays, DNA KW - Arrays, Oligonucleotide KW - Arrays, cDNA KW - Chip, DNA KW - Chip, Gene KW - Chips, DNA KW - Chips, Gene KW - DNA Array KW - DNA Chip KW - DNA Microarray KW - DNA Microchip KW - Gene Chip KW - Microarray, DNA KW - Microarray, Oligonucleotide KW - Microarray, cDNA KW - Microarrays, DNA KW - Microarrays, Oligonucleotide KW - Microarrays, cDNA KW - Microchip, DNA KW - Microchips, DNA KW - Oligonucleotide Array KW - Oligonucleotide Microarray KW - cDNA Array KW - cDNA Microarray KW - Oligonucleotide Probes KW - Combinatorial Chemistry Techniques KW - Gene Expression Profiling KW - DNA biochips KW - Biochips KW - Immobilized nucleic acids KW - instrumentation KW - Gene Expression Microarray Analysis UR - https://www.unicat.be/uniCat?func=search&query=sysid:25709634 AB - DNA microarrays, or biochips, are small glass chips embedded with ordered rows of DNA and by providing a massive parallel platform for data gathering represent a fundamental technical advance in biomedical research. Such biochips enable the use of advanced fabrication, detection, and data mining technologies that allow data gathering at an unprecedented rate. The first chapter is an introduction to the technology of DNA microarrays, emphasizing the methodological fundamentals of biochips. The next two chapters describe the use of confocal scanning in microarray detection and techniques for the efficient cloning and screening of differentially expressed genes. Chapter 4 describes assay optimization for enzymatic assays and chapter 5 antisense scanning arrays. This is followed by a chapter on the manufacture of molecular arrays using ink-jet printing technology. Chapter 7 describes gene expression analysis from cDNA microarrays and then chapter 8 covers the use of expression data in bioinformatics. Chapter 9 describes the use of active microelectronic arrays for DNA hybridization analysis and the last chapter details the use of microarray technology in pharmacogenomics. This Practical Approach book is a comprehensive overview of the new and expanding field of DNA microarray technology and will be invaluable to any researcher interested in the use of biochips. ER -